Human urine-derived stem cells transplantation for treatment of myocardial infarction in rats

doi:10.3969/j.issn.2095-4344.0664

Abstract

Abstract:

BACKGROUND: In recent years, many studies have shown that bone marrow mesenchymal stem cells, adipose-derived stem cells, and cardiac stem cells can participate in myocardial repair, but little is reported on the use of human urine-derived stem cells in the treatment of myocardial infarction.
OBJECTIVE: To investigate the effect of human urine-derived stem cell transplantation on the heart function of myocardial infarction rats.
METHODS: The 20 of 64 Sprague-Dawley rats were selected as normal control group, and the remaining 44 rats were subjected to ligation of the left anterior descending coronary artery to make myocardial infarction models. The 40 model rats were successfully made and randomized into myocardial infarction group and cell transplantation group with 20 rats in each group. The rats in the myocardial infarction group were given injection of normal saline by tail vein, while those in the cell transplantation were given injection of human urine-derived stem cells labeled by CM-Dil by tail vein. The treatment was done once a day and lasted for 3 consecutive days. At 6 weeks after cell transplantation, the heart function of rats was examined by echocardiography, and the myocardial tissues of rats in each group were taken for CD34 immunohistochemical staining, hematoxylin-eosin staining, TUNEL staining and RT-PCR detection.
RESULTS AND CONCLUSION: Compared with the myocardial infarction group, significantly improved heart function of rats, increased density of neonate blood capillaries, and decreased apoptotic rate were observed in the cell transplantation group (all P < 0.05). The CM-Dil positive cells were not observed in the normal control group and myocardial infarction group, while a great number of positive cells appeared in the cell transplantation group. Compared with the myocardial infarction group, Bcl-2 gene expression level in the cell transplantation group was significantly higher (P < 0.05), while the Caspase-3 gene expression level was lowered (P < 0.05). In conclusion, transplantation of human urine-derived stem cells can significantly improve the heart function of rats with acute myocardial infarction, and reduce cell apoptosis in the rat myocardium.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Urine, Stem Cell Transplantation, Myocardial Infarction, Tissue Engineering

CLC Number:

R394.2

Sun He-yuan. Human urine-derived stem cells transplantation for treatment of myocardial infarction in rats[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(33): 5303-5308.

Figures/Tables 2

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