A comparative study on biological characteristics of mesenchymal stem cells from two different sources

doi:10.3969/j.issn.2095-4344.0500

Abstract

Abstract:

BACKGROUND: Although mesenchymal stem cells (MSCs) from different sources share many similar characteristics, they also exhibit individual properties.
OBJECTIVE: To compare the biological characteristics of MSCs derived from umbilical cord and decidua parietalis.
METHODS: Growth curve, cell doubling time, clone formation rate, immune phenotype, differentiation capacity and secreted cytokine levels were analyzed in MSCs derived from umbilical cord and decidua parietalis.
RESULTS AND CONCLUSION: MSCs from umbilical cord and the decidua basalis exhibited similar morphology, spiral growth, S-shaped growth curve, immunophenotype, and differentiation potentials to osteogenesis and adipogenesis. For two kinds of MSCs, the positive rates of CD73, CD90 and CD105 were over 95% and the positive rates of CD34 and CD45 were below 1%. The growth rate, cell doubling time and clone formation rate of umbilical cord derived MSCs at passages 2 and 5 were significantly higher than those of decidua parietalis derived MSCs at passages 2 and 5 (P < 0.05). The level of epidermal growth factor secreted from umbilical cord MSCs was significantly higher that that from decidua basalis derived MSCs, while the levels of vascular endothelial growth factor and stem cell growth factor from umbilical cord derived MSCs was significantly lower those from decidua basalis derived MSCs (P < 0.05). These findings indicate that MSCs from both sources have similar biological properties, but umbilical cord derived MSCs are deemed to have better application prospects.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Mesenchymal Stem Cells, Umbilical Cord, Decidua, Tissue Engineering

CLC Number:

R394.2

Wang Gang, Li Dong-sheng, Guan Yu-tao, Zhu Yu-yuan, Liao Pin-fu, Xu Jun-rong, Chen Zhi-cong . A comparative study on biological characteristics of mesenchymal stem cells from two different sources[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(17): 2705-2710.

Figures/Tables 3

2.1 脐带间充质干细胞和壁蜕膜间充质干细胞的形态从两种不同组织中得到较均一的间充质干细胞，两种细胞均为梭形成纤维样细胞，漩涡式生长(图1)，但壁蜕膜间充质干细胞的长度明显长于脐带间充质干细胞。 2.2 脐带间充质干细胞和壁蜕膜间充质干细胞的增殖能力比较 2.2.1 脐带间充质干细胞和壁蜕膜间充质干细胞的生长曲线两种间充质干细胞的生长曲线均为S型。在同一时间段，第2代和第5代的脐带间充质干细胞的数量均多于壁蜕膜间充质干细胞，随着代数的增长，细胞增殖速度逐渐减慢(图2)。 2.2.2 脐带间充质干细胞和壁蜕膜间充质干细胞的倍增时间如图3所示，第2代脐带间充质干细胞的倍增时间为(17.73±0.51) h，第2代壁蜕膜间充质干细胞的倍增时间为(21.93±0.72) h，两者比较差异有显著性意义(P < 0.05)。第5代脐带间充质干细胞的倍增时间为(14.50±0.81) h，第5代壁蜕膜间充质干细胞的倍增时间为(19.63±1.1) h，两者比较差异有显著性意义(P < 0.05)。 2.2.3 脐带间充质干细胞和壁蜕膜间充质干细胞的克隆形成率两种间充质干细胞的克隆着色较为明显，克隆面积较大。第2代脐带间充质干细胞的克隆形成率为(16.00±1.41)%，第2代壁蜕膜间充质干细胞的克隆形成率为(13.60±1.50)%，两者比较差异有显著性意义(P < 0.05)。第5代脐带间充质干细胞的克隆形成率为(12.80±0.75)%，第5代壁蜕膜间充质干细胞的克隆形成率为(8.60±1.85)%，两者比较差异有显著性意义(P < 0.05)，见图4。 2.3 脐带间充质干细胞和壁蜕膜间充质干细胞的免疫表型分析第3代脐带间充质干细胞和壁蜕膜间充质干细胞表面CD73、CD90和CD105表达阳性，大于95%，而CD34、CD45表达阴性，小于1%，见图5和表1。 2.4 脐带间充质干细胞和壁蜕膜间充质干细胞的诱导分化 2.4.1 成脂诱导能力两种来源间充质干细胞成脂诱导14 d，油红O染色后出现红色脂滴(图6)。 2.4.2 成骨诱导能力两种来源间充质干细胞成骨诱导14 d，经茜素红染色可见细胞爬片上出现红色钙结节(图7)。 2.5 脐带间充质干细胞和壁蜕膜间充质干细胞分泌的细胞因子水平脐带间充质干细胞分泌的表皮生长因子水平显著高于壁蜕膜间充质干细胞(P < 0.05)；脐带间充质干细胞分泌的血管内皮生长因子和干细胞生长因子水平显著低于壁蜕膜间充质干细胞(P < 0.05)；脐带间充质干细胞和壁蜕膜间充质干细胞上清液中碱性成纤维细胞生长因子、角质细胞生长因子和转化生长因子β水平差异无显著性意义(P > 0.05)，见表2。"

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