Effect of acupuncture at Zusanli on T lymphocyte subsets in mesenteric lymph nodes of spleen deficiency syndrome rats

doi:10.3969/j.issn.2095-4344.0093

Abstract

Abstract:

BACKGROUND: Intestinal and lymphoid tissues constitute an important part of intestinal immunity, which plays an important regulatory role in spleen deficiency and hydronephrosis.
OBJECTIVE: To observe the effect of acupuncture on T lymphocyte subsets in lymph nodes of rats with spleen deficiency, and to investigate the correlation of spleen deficiency with intestinal immunity and the mechanism of acupuncture for spleen deficiency syndrome.
METHODS: Thirty-six female Sprague-Dawley rats were randomly divided into three groups: model, acupuncture and blank control groups. The rat model of spleen deficiency was established by fatigue-induced spleen injury plus abnormal diet for 31 days. Afterwards, the rats in the acupuncture group received acupuncture at Zusanli (ST 36). Urine D-xylose excretion rate was detected during modeling and treatment. Then, the mesenteric lymph nodes were removed, and the changes in T lymphocyte subsets in the mesentericlymph nodes were observed by immunohistochemistry.
RESULTS AND CONCLUSION: Urine D-xylose excretion rate under spleen deficiency in the modeling and acupuncture groups was significantly lower than that in the blank control group (P < 0.05 or P < 0.01); after acupuncture, the urine D-xylose excretion rate was significantly increased compared with the modeling group (P < 0.01), but still lower than that in the blank control group (P < 0.05). The count of CD4+ T lymphocytes, count of CD8+ T lymphocytes and ratio of CD4+/CD8+ T lymphocytes were ranked as follows: blank control group > acupuncture group > modeling group (P < 0.01 or P < 0.05). These results suggest that acupuncture at Zusanli can improve the urine D-xylose excretion rate, regulate the balance of T lymphocyte subsets in mesenteric lymph node of rats with spleen deficiency, thus improving the intestinal immune function, spleen deficiency systems, disorder of intestinal digestive function, intestinal digestion and absorption, as well as anorexia, loose stool, diarrhea and other symptoms of the digestive system.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: the Acupuncture, Mesentery, Lymph Nodes, T-Lymphocyte Subsets, Tissue Engineering

CLC Number:

R318

Zeng Rong-hua, Zhou Lu, Ouyang Hou-gan, Peng Shan, Gao Shu-liang, Wang Jian-min, Wu Hui-ting, Ouyang Yan-chu, Cui Tian-tian. Effect of acupuncture at Zusanli on T lymphocyte subsets in mesenteric lymph nodes of spleen deficiency syndrome rats[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(4): 576-581.

Figures/Tables 2

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