Preparation of a controlled-release icariin/beta-tricalcium phosphate composite scaffold

doi:10.3969/j.issn.2095-4344.0060

Abstract

Abstract:

BACKGROUND: Icariin can promote bone formation and inhibit bone resorption, but it is difficult to dissolve in water, and its bioavailability is very low in vivo. Therefore, a suitable carrier is essential to fully utilize the biological activity of icariin.

OBJECTIVE: To prepare an icariin/β-tricalcium phosphate (β-TCP) composite scaffold and to characterize its biological characteristics.

METHODS: Three-dimensional printing technology was utilized to prepare porous β-TCP scaffolds carrying nano zinc oxide, and the compressive strength of porous β-TCP scaffolds was detected before and after addition of nano zinc oxide. Water absorption and porosity of the porous β-TCP scaffolds were also measured. Ultrasonic emulsification solvent dialysis was performed to prepare icariin/ poly(lactic-co-glycolic acid) (PLGA) microspheres, followed by the detection of water absorption and porosity. The porous β-TCP scaffolds and icariin/PLGA microsphere suspension were mixed to prepare icariin/β-TCP composite scaffolds. Microstructure observation of the composite scaffolds was done by scanning electron microscope, and meanwhile, water absorption and porosity were detected. The composite scaffold was then immersed into PBS, and icariin concentration in the supernatant was measured at corresponding time points, based on which Icariin cumulative release curve was drawn.

RESULTS AND CONCLUSION: (1) The porous microstructure of the porous β-TCP scaffold was regular, well distributed, and the connectivity was good. The pore spacing was about 600 μm. After addition of nano zinc oxide particles, the surface structure of the scaffold was more compact and the crystallinity was higher. (2) PLGA microspheres were spherical with a diameter of 1-4 μm, and the microspheres were uniform in size. (3) The maximum compressive strength of the porous beta tricalcium phosphate scaffold was (2.98±0.78) MPa, and increased to (8.95±0.29) MPa after addition of nano zinc oxide. (4) The water absorption rate and porosity were (25.09±0.96)% and (66.93±2.84)% for the porous β-TCP scaffold, (28.46±1.85)% and (32.65±3.32)% for the icariin/β-TCP composite scaffold, respectively. (5) The average encapsulation efficiency of the PLGA microspheres was (78.87±2.31)%, and the drug loading was (6.04±1)%. (6) The release amount of icariin could reach 52% of the total amount at 16 days and 60% of the total amount at 32 days. These findings indicate that the icariin/β-TCP composite scaffold has good mechanical properties and sustained-release performance.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Calcium Phosphates, Drugs, Chinese Herbal, Delayed-Action Preparations, Tissue Engineering

CLC Number:

R318

Xue Peng, Du Bin, Wang Li-ning, Cao Liang-quan, Sun Guang-quan, Liu Xin, Yu Heng-heng. Preparation of a controlled-release icariin/beta-tricalcium phosphate composite scaffold[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(6): 865-870.

Figures/Tables 7

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