Abstract:

BACKGROUND: Studying the gene polymorphism of autosome short tandem repeat (STR) gene loci can provide basic data for forensic paternal power identification. 
OBJECTIVE: To explore the genetic polymorphism of three STR loci, D16S539, THO1, D13S317, in Liaoning Xibo population of China and establish the genetic basic data of Xibo population.
METHODS: Buccal samples were obtained from 150 primary and middle school students of Xibo population living in Liaoning province and PCR amplification reactions of each STR loci were performed in fluorescence-based reaction using a 2720 Thermal cycler. The amplified products were detected in a Li-COR 4300 DNA Analyzer. Relative fragment size of amplified products was calculated through the use of E-seq Analysis software and genotyping was performed. Allele frequency of three STR genetic loci of Xibo population living in Liaoning province was investigated and genetic polymorphism analysis was performed.
RESULTS AND CONCLUSION: All the three loci met the Hardy-Weinberg equilibrium. Heterozygosis degree of these three loci was distributed between 0.769-0.810. Individual discriminative power was distributed between 0.824-0.929. Cumulative individual discriminative power was 0.999. Polymorphism information was distributed between 0.650-0.790. Non-parental eliminant rate was distributed between 0.565-0.790. Cumulative non-parental eliminant rate was 0.979. The three STR loci used in this study show high non-parental eliminant rate and individual discriminative power in Xibo population and can be applied to population study and forensic practice.