Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (53): 9881-9886.doi: 10.3969/j.issn.2095-4344.2012.53.001

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Y-chromosome in situ hybridization technology for detection of transplanted hepatic stem cells in the liver graft

Li Zhu1, Chen Juan1, Li Xue-hua1, Liu Zhi-heng1, Liu Gui-jie1, Zhang Xue-li1, Gao Yan-chao1, Sun Hui-dong1,Ji Pi- you1,Zhang Jin-liang1, Li Li2   

  1. 1Department of Hepatobiliary Surgery, the People’s Hospital of Liaocheng (Liaocheng Hospital Affiliated to Taishan Medical University), Liaocheng 252000, Shandong Province, China
    2First Ward, Department of Hepatobiliary Surgery, the First People’s Hospital of Kunming (Ganmei Hospital Affiliated to Kunming Medical University), Kunming 650011, Yunnan Province, China
  • Received:2012-04-06 Revised:2012-06-12 Online:2012-12-30 Published:2012-12-30
  • Contact: Li Li, Professor, Doctoral supervisor, First Ward, Department of Hepatobiliary Surgery, the First People’s Hospital of Kunming (Ganmei Hospital Affiliated to Kunming Medical University), Kunming 650011, Yunnan Province, China ynkmlili@yahoo.com.cn
  • About author:Li Zhu☆, Doctor, Attending physician, Department of Hepatobiliary Surgery, the People’s Hospital of Liaocheng (Liaocheng Hospital Affiliated to Taishan Medical University), Liaocheng 252000, Shandong Province, China lllllzhu@126.com

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Abstract:

BACKGROUND: The colonization tracer technology after stem cells transplantation includes magnetic probe labeling, fluorescent tracer method, lentivirus infections fluorescent labeling and Y chromosome in situ hybridization technology, and each method has its own disadvantages and advantages.
OBJECTIVE: To observe the transplantation conditions of hepatic stem cells (hepatic oval cells) in the liver graft by Y-chromosome in situ hybridization technology.
METHODS: Rat orthotropic liver transplantation models were established by using Kamada two-cuff technique. Female DA rats served as donors, and 60 female Lewis rats were used as recipients. Hepatic oval cells was originated from male Lewis rats and implanted to the liver graft during liver transplantation. Y-chromosome in situ hybridization technology was used to detect hepatic stem cells transplantation conditions in the liver graft.
RESULTS AND CONCLUSION: Hepatic oval cells and differentiated cells mainly distributed around central vein area and portal area in the liver graft. The transplantation conditions of recipient derived hepatic stem cells in the liver graft were effectively detected by Y-chromosome in situ hybridization technology.

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