Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (53): 9898-9902.doi: 10.3969/j.issn.2095-4344.2012.53.004

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Protective effect of gdolinium chloride against ischemia-reperfusion injury to the transplanted liver

Quan Hu1, He Zhi-jun2, Zuo Chao-hui1, Ouyang Yong-zhong1, Xu Mi3, Tang Ming1, Tang Wei1, Wang Yi-chun1   

  1. 1Department of Gastroduodenal Pancreatic Surgery, Hunan Provincial Tumor Hospital, Changsha 410011, Hunan Province, China
    2Department of Organ Transplantation of General Surgery, the Xiangya Second Hospital of Central South University, Changsha 410011, Hunan Province, China
    3Department of Hepatobiliary Surgery, Hunan People's Hospital, Changsha 410011, Hunan Province, China
  • Received:2012-03-19 Revised:2012-05-15 Online:2012-12-30 Published:2012-12-30
  • Contact: He Zhi-jun, Doctor, Professor, Chief physician, Master’s supervisor, Department of Organ Transplantation of General Surgery, the Xiangya Second Hospital of Central South University, Changsha 410011, Hunan Province, China
  • About author:Quan Hu★, Master, Physician, Department of Gastroduodenal Pancreatic Surgery, Hunan Provincial Tumor Hospital, Changsha 410011, Hunan Province, China Quanhu2009@sina.com

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Abstract:

BACKGROUND: Gadolinium chloride can inhibit the Kupffer cell activation, reduce their phagocytic activity, and reduce tumor necrosis factor alpha and interleukin 1 release after Kupffer cell activation, thereby alleviate the ischemia-reperfusion injury of liver.
OBJECTIVE: To study the effect of gdolinium chloride on ischemia-reperfusion injury of rat receptor’s liver and to explore the mechanism.
METHODS: Donors and receptors were all male Sprague Dawley rats which were randomly divided into three groups. The modified Kamada’s two-cuff technique was used to build rat liver transplantation model through liver superior and inferior vena cava reconstruction by modified continuous suture. In the sham-operation group, the rats were treated without liver transplantation, the liver was freed and the abdomen was closed after vein ligation without treatment and received drug administration. In the saline group, the warm ischemia time was 0-5 minutes and cold preservation time of the donor liver was 2 hours, the saline was injected into the rats via the tail vein for 3 days before implantation, and after implantation, the saline was injected into the rats once more. In the gadolinium chloride group, the warm ischemia time was 0-5 minutes and cold preservation time of the donor liver was 2 hours, 0.5% gadolinium chloride was injected into the rats via the tail vein for 3 days before implantation, and after implantation, the injection was performed once more. The rats were sacrificed after 24 hours for the corresponding index detection.
RESULTS AND CONCLUSION: Compared with sham-operation group, the serum alanine aminotransferase, alanine aminotransferase, alkaline phosphatase and γ-gamma-glutamyl transferase levels were increased significantly in gadolinium chloride and saline groups (P < 0.05); all the indexes in gadolinium chloride group were decreased compared with saline group (P < 0.05). In pathologic histology tests, the lesion range and the extent of ischemia-reperfusion injury were all significantly increased in saline group and gadolinium chloride group. Compared with the saline group, the concentrations of interleukin-1, tumor necrosis factor-α and the apoptosis index were decreased significantly in gadolinium chloride group (P < 0.05), as well as the congestion, vacuolar degeneration and Suzuki’s score (P < 0.05). The protective effect of gdolinium chloride on ischemia-reperfusion injury of receptor’s liver might result from closing the Kupffer cells and reducing cytokine release.

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