Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (44): 8301-8304.doi: 10.3969/j.issn.1673-8225.2011.44.033

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Human leukocyte antigen allele and haplotype in 56 cases of hematopoietic stem cell transplantation

Liu Chuan1,2, Wan Hua3, Zou Ye-qing1, Li Jian1, Li Guo-liang4, Fu Ying-yuan2   

  1. 1Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang   330006, Jiangxi Province, China
    2Immunization Department, Medical College of Nanchang University, Nanchang   330006, Jiangxi Province, China
    3Laboratory of the First Hospital of Nanchang, Nanchang  330008, Jiangxi Province, China
    4tissue Matching Laboratory of Jiangxi Province Blood Center, Nanchang 330077, Jiangxi Province, China
  • Received:2011-04-08 Revised:2011-06-23 Online:2011-10-29 Published:2011-10-29
  • Contact: Fu Ying-yuan,Professor, Master’s supervisor, Immunization Department, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China hqfyy@126.com
  • About author:Liu Chuan★, Studying for master’s degree, Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; Immunization Department, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China lc61116@163.com

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Abstract:

BACKGROUND: It has great significance to understand individual human leucocyte antigen allele (HLA) haplotype and genotype.
OBJECTIVE: To analyze HLA allele frequency, haplotype frequency and characteristics in 56 cases of hematopoietic stem cell transplantation.
METHODS: HLA-A, B, DRB1 antigen was subjected to genotyping using PCR-SSP technique. The allele and haplotype frequencies of HLA-A, B, DRB1 were calculated.
RESULTS AND CONCLUSION: A total of 8 HLA-A, 19 HLA-B and 13 HLA-DRB1 alleles were detected among 56 cases, showing a rich polymorphism. Among 224 haplotypes, A﹡02-B﹡46, A﹡11-B﹡40, B﹡46-DRB1﹡09 were informative with frequency higher than 0.10. A total of 10 HLA-A-B haplotypes and 4 HLA-B-DRB1 haplotypes were in strongest linkage disequilibrium. Results showed that HLA alleles have richer polymorphisms with obvious geographic genetic characteristics.

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