Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7515-7519.doi: 10.3969/j.issn.1673-8225.2011.40.024

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In vivo tracking superparamagnetic iron oxide labeled adipose derived stem cells to repair rabbit degenerated intervertebral disc

Jiang Xin-hua1, Chen Jian-yu2, Cai Zhao-xi2, Zhang Ya2, Liu Zhen-zhen2, Xie Chuan-miao1, Wu Yao-pan1, Wu Pei-hong1   

  1. 1State Key Laboratory of Oncology in South China, Department of Medical Imaging and Interventional Radiology, Cancer Center of Sun Yat-sen University, Guangzhou  510060, Guangdong Province, China
    2Radiology Department of Sun Yat-sen Memorial Hospital, Guangzhou  510120, Guangdong Province, China
  • Received:2011-03-16 Revised:2011-05-18 Online:2011-10-01 Published:2011-10-01
  • Contact: Chen Jian-yu, Professor, Master’s supervisor, Radiology Department of Sun Yat-sen Memorial Hospital, Guangzhou 510120, Guangdong Province, China
  • About author:Jiang Xin-hua★, Master, Physician, State Key Laboratory of Oncology in South China, Department of Medical Imaging and Interventional Radiology, Cancer Center of Sun Yat-sen University, Guangzhou 510060, Guangdong Province, China 170768357@qq.com
  • Supported by:

    the Science and Technology Research Project of Guangdong Province, No. 2008B030301318*

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Abstract:

BACKGROUND: Numerous studies have demonstrated fluorescent labeled bone marrow mesenchymal stem cells transplantation, but few reports have addressed in vivo tracking of superparamagnetic iron oxide (SPIO) labeled adipose derived stem cells (ADSCs) and its contribution to repair degenerated intervertebral disc.
OBJECTIVE: To In vivo track transplanted SPIO labeled ADSCs survival and migration by MRI, and to investigate the contribution of ADSCs to repair degenerative intervertebral disc.
METHODS: Twenty rabbits were divided into four groups: normal group (L1/2), ADSCs group (L2/3), PBS group (L3/4), SPIO-labeled ADSCs group (L4/5). X-ray photograph, MR scanning and histopathological examination were done in time interval after SPIO labeled ADSC was transplanted.
RESULTS AND CONCLUSION: SPIO labeled ADSCs transplanted intervertebral disc showed low signal intensity on T2WI/FFE, and low signal intensity remained on T2WI/FFE even after 8 weeks in transplanted SPIO labeled ADSC intervertebral disc. Compared with the PBS group, ADSCs group showed a lesser extent degeneration. SPIO labeled ADSCs transplanted intervertebral disc can be tracked by MRI; ADSCs transplantation is effective in regeneration of degenerated intervertebral disc.

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