Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7511-7514.doi: 10.3969/j.issn.1673-8225.2011.40.023

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In vitro differentiation of mesencephalic neural stem cells induced by glial-derived neurotrophic factor under hypoxia

Ding Ji-gu1, Ding Wen-jie2   

  1. 1Department of Human Anatomy, 2Department of Stomatology, Medical School of Xianning University, Xianning  437100, Hubei Province, China
  • Received:2011-04-18 Revised:2011-04-25 Online:2011-10-01 Published:2011-10-01
  • About author:Ding Ji-gu, Professor, Master’s supervisor, Department of Human Anatomy, Medical School of Xianning University, Xianning 437100, Hubei Province, China dingjigu@hotmail.com Ding Wen-jie, Master, Department of Stomatology, Medical School of Xianning University, Xianning 437100, Hubei Province, China Ding Ji-gu and Ding Wen-jie contributed equally to this paper.
  • Supported by:

    the Key Project of Hubei Education Bureau, No. D20092802*

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Abstract:

BACKGROUND: During neural stem cell transplantation in the treatment of Parkinson’s disease, the number of transplanted cells and differentiation ratio of dopaminergic neurons must be resolved. Effective in vitro proliferation of neural stem cells and large amount of directed differentiation of dopaminergic neurons are the key to solve above-mentioned problems.
OBJECTIVE: To investigate the differentiation of mesencephalic neural stem cells into dopaminergic neurons during the hypoxia induced by glial cell line-derived neurotrophic factor (GDNF) in vitro.
METHODS: Ventral midbrain tissue isolated from embryonic mice of pregnant 12 days was made into single cell suspension and cultured in non-serum medium containing basic fibroblast growth factor (bFGF) and B27, and then proliferated under normoxia or hypoxia for 5-7 days followed by incubation in DMEM/F12 medium containing 10% fetal bovine serum or 10% fetal bovine serum + 1 g/L GDNF.
RESULTS AND CONCLUSION: Under the hypoxia environment, the number of dopaminergic neurons differentiated from mesencephalic neural stem cells especially induced by GDNF was higher than that under normoxia. It indicated that under the hypoxia environment, GDNF can induce mesencephalic neural stem cells differentiating into dopaminergic neurons with enough quantity, mature shape and function.

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