Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2657-2660.doi: 10.3969/j.issn.1673-8225.2011.14.042

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Effects of high glucose and advanced glycation end-products on osteogenic differentiation of human adipose-derived stromal cells in vitro

Li Dong-song1, Li Shu-qiang1, Cai Bo2, Wang Ping1, Feng Wei1, Liu Jian-guo1   

  1. 1Department of Orthopedics, the First Hospital of Jilin University, Changchun  130021, Jilin Province, China
    2Department of Special Diagnosis, the 208 Hospital of Chinese PLA, Changchun  130061, Jilin Province, China
  • Received:2010-11-11 Revised:2011-01-02 Online:2011-04-02 Published:2013-11-02
  • Contact: Liu Jian-guo, Doctor, Professor, Department of Orthopedics, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China jgliu.2005@yahoo.com.cn
  • About author:Li Dong-song☆, Doctor, Attending physician, Department of Orthopedics, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China Armstrong586@163.com
  • Supported by:

    the Youth Foundation of Science and Technology Commission of Jilin Province, No. 20090130*

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Abstract:

BACKGROUND: Bone metabolism disorder happens in diabetic environment, bone defects in which are difficult to repair. Study addressing osteogenic property of adipose-derived stroma cells (ADSCs) in diabetic environment provides theoretical basis for its application in certain environment.
OBJECTIVE: To explore the effects of high glucose (HG) and advanced glycation end-products (AGEs) on osteogenic capacity of human ADSCs.
METHODS: 100 mg/L AGEs and 27.5 mmol/L HG were used to simulate in vitro diabetic environment and intervened ADSCs osteogenic differentiation. The cells were divided into 4 groups, with 6 samples in each group. The expression of type Ⅰ collagen was examined by fluorescent immunofluorescence at 21 days after osteogenic induction. The number of calcification nodes was counted under contrast phase microscopy at 14, 21 and 28 days. 
RESULTS AND CONCLUSION: Fluorescent quantitation scan showed that the type Ⅰ collagen amount of the AGEs+HG treated group was 2.76 times lower than that of the control group. AGEs+HG reduced the number of ADSCs calcification nodes compared with the control, HG, and AGEs groups, the differences were statistical significant (P < 0.01). AGEs and HG exposure inhibit the cognate osteogenic differentiation of ADSCs, which suggest that AGEs and HG are unfavorable factors that reduce ADSCs osteogenic ability. 

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