Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2597-2600.doi: 10.3969/j.issn.1673-8225.2011.14.027

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Differentiation of human dermal-derived multipotent stem cells into epidermal cells in diabetic wound

Gao Xin-yu1, Zhao Li-ping1, Ke Chang-neng2, Wang Ming-gang1, Zhong Xiao-hong1   

  1. 1Department of Plastic Surgery, the Affiliated Provincial Hospital of Anhui Medical University, Hefei  230001, Anhui Province, China
    2Department of Burn and Plastic Surgery, Second Affiliated Hospital of Medical School of Shantou University, Shantou  515041, Guangdong Province, China
  • Received:2010-10-20 Revised:2011-03-07 Online:2011-04-02 Published:2013-11-02
  • Contact: Zhao Li-ping, Doctor, Associate chief physician, Department of Plastic Surgery, the Affiliated Provincial Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China zhlipl6@yahoo.com.cn
  • About author:Gao Xin-yu★, Studying for master’s degree, Department of Plastic Surgery, the Affiliated Provincial Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China gaoxinyu@126.com
  • Supported by:

    the Natural Science Foundation of Anhui Education Bureau, No. KJ2008B297*; Science and Technology Foundation of Anhui Health Bureau, No. 09A028*; Anhui Natural Science Foundation, No. 11040606M161*; Provincial Science and Technology Program of Anhui Universities, No. KJ2011Z205*, KJ2008B297*

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Abstract:

BACKGROUND: Differentiation of stem cells is closely related to the microenvironment. Therefore, it is assumed that human dermal-derived multipotent stem cells may have a potential to differentiate into epidermal cells in skin wound.
OBJECTIVE: To explore the feasibility of human dermal-derived multipotent stem cells differentiating into epidermal cells under the microenvironment of diabetic wound.
METHODS: Human dermal-derived multipotent stem cells were isolated and cultured. Diabetic naked mouse models were prepared. The third to fifth generation of human dermal-derived multipotent stem cells labeled with 5-bromodeoxyuridine (5-BrdU) were injected into the skin tissue surrounding the wound of diabetic mouse models. The specimens were harvested from the wound tissues for paraffin embedding, and serial sections were made at the 2nd and 3rd week after transplantation for BrdU and keratin staining.
RESULTS AND CONCLUSION: Not only BrdU positive cells aggregated in the epidermis but also some positive cells expressed keratin simultaneously in the epidermis. During the course of diabetic wound healing, human dermal-derived multipotent stem cells have the potential to differentiate into epidermal cells.

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