Abstract:

BACKGROUND: The ganglioside is the only world-recognized repair nerve damage, remodeling sovereign remedy of neural network. Monosialoganglioside (GM1) is one kind of ganglioside.
OBJECTIVE: To observe the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation combined with GM1 on the treatment of cerebral infarction in rats.
METHODS: The middle cerebral artery occlusion (MCAQ) models in Wistar rats were established by suture method, which were randomly divided into 3 groups: cell transplantation group, infarction group and cell transplantation + GM1 group. The concentration of 1×1010/L BMSCs suspension or the concentration of 1 mL cell culture fluid was injected into MCAQ models through caudal vein, at the same time, GM1 aqueous solution or normal saline was injected into MCAQ models through abdominal cavity, once a day, for 3 days. Longa ethology scores were determined at 24 hours, 3 days after vein transplantation, and 1, 2 weeks after MCAQ. Neurological damage was detected. After 3 days treatment, RT-PCR and Western Blot was used to detect changes of AQP4 mRNA expression and protein synthesis. After 2 weeks, the survival of implanted cells and the repair of tissue were observed by BrdU immunohistochemistry and hematoxylin-eosin staining.
RESULTS AND CONCLUSION: The neurological dysfunction scores in cell transplantation group + GM1 group were significantly lower than those in cell transplantation group and infarction group at 1 and 2 weeks after transplantation (P < 0.05). At 3 days after transplantation, AQP4 protein in the surrounding tissue cerebral infarction and its mRNA expression in infarction group were higher than those in cell transplantation and cell transplantation + GM1 groups (P < 0.05). The number of neurons in BrdU immunohistochemistry and hematoxylin-eosin staining slice in cell transplantation + GM1 group was higher than that in cell transplantation and infarction groups (P < 0.05). The results suggested that BMSCs transplantation combined with GM1 can significantly improve neurological function of cerebral infarction in rats.