Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2551-2556.doi: 10.3969/j.issn.1673-8225.2011.14.018

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Treatment of traumatic brain injury in rats with intravenous transplantation of adipose-derived stem cells

Zhang Tian-xiang1, 2, Yang Bo1, 2, Guan Fang-xia3, Hu Xiang4, 5, Du Ying6, Qiao Xiao-jun1, Tian Yi1, Ba Yun-tao1, Duan Xiao-bing1, Deng Xiao-hui1, Gu Chen-xi7, Zheng Wen-di7   

  1. 1Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    2 Key Discipline Open Laboratories of Clinical Medicine, Henan High Education, Zhengzhou  450052, Henan Province, China
    3Department of Bioengineering, Zhengzhou University, Zhengzhou  450001, Henan Province, China
    4Jiangsu Public Technology Service Platform of Stem Cells and Biotherapy, Taizhou  225300, Jiangsu Province, China
    5Shenzhen Beike Cell Engineering Institute, Shenzhen  518057, Guangdong Province, China
    6Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    7Department of Clinical Medicine, Zhengzhou University, Zhengzhou  450052, Henan Province, China
  • Received:2010-12-01 Revised:2011-01-13 Online:2011-04-02 Published:2013-11-02
  • Contact: Yang Bo, Master, Professor, Doctoral Supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Key Discipline Open Laboratories of Clinical Medicine, Henan High Education, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Zhang Tian-xiang★, Studying for master's degree, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Key Discipline Open Laboratories of Clinical Medicine, Henan High Education, Zhengzhou 450052, Henan Province, China zhangtianxiang8005@126.com Guan Fang-xia, Doctor, Professor, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China guanfangxia@126.com Zhang Tian-xiang and Guan Fang-xia contributed equally to this paper.
  • Supported by:

    211 Project-Phase Ⅲ- the Basic and Clinical Research of Stem Cells of Zhengzhou University*; Jiangsu Provincial Public Technology Service Platform of Stem Cells and Biotherapy, No. BM2008146*

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Abstract:

BACKGROUND: Animal experiments and clinical researches have confirmed that stem cells have a therapeutic effect on the traumatic brain injury.
OBJECTIVE: To explore the behaviors of rats of acute traumatic brain injury and expressions of glial fibrillary acidic protein (GFAP), neuronspecific enolase (NSE), and nidogen in brain tissue for the treatment of adipose-derived stem cells (ADSCs) transplantation.
METHODS: Brain injury model of SD rats were established by free-fall impact method of epidural, and were randomly divided into 3 groups: control group, transplantation group, and saline group. Brdu-labled ADSCs underwent caudal vein injection at 48 hours brain injury in transplantation group; any treatment was not given for the control group; saline underwent caudal vein injection at 48 hours brain injury in saline group. Nerological severiyt score (NSS) method was applied to evaluate the recovery of neurological function in rats. Migration of ADSCs in brain injury tissue was observed by inverted microscope. The expressions of GFAP, NSE, and nidogen in damaged brain tissue of rats were detected by using of immunohistochemistry method.
RESULTS AND CONCLUSION: NSS score in transplantation was significantly higher than that in control and saline groups (P< 0.05). Brdu-labled ADSCs could be found in damaged brain tissue. The expression of GFAP and NSE in transplantation group was significantly lower than that in control and saline groups in damaged brain tissue of rats (P < 0.05), and the rate of decay was rapid. The expression of nidogen in transplantation was significantly higher than that in control and saline groups (P< 0.05). It was confirmed that ADSCs through the blood-brain barrier into the damaged brain tissue to protect neuron, and promote the function of neural development and regeneration in terms of up-regulation of nidogen gene expression.

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