Abstract:

BACKGROUND: Adipose tissue-derived stem cells (ADSCs) have multiple differentiation potentials, which can differentiate into chondrocytes, osteocytes, adipose cells, and cardiac muscle cells under different induced conditions, in addition, they can also differentiated into nerve cells. 
OBJECTIVE: To research the in vitro culture and phenotype of rat ADSCs, and to explore it differentiation potential into nerve cells. 
METHODS: Inguinal, epididymis and post peritoneal fat tissues were gotten from rats, ADSCs were isolated and cultivated in media, and their shape was recorded passaged. The third or the forth passage cells were identified by stem cells common marker CD29, CD44 and CD45. ADSCs were induced to nerve cells in a simulated nerve cells habitat with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and then expressions of the neuron specific markers nestin, NF-200 and GFAP were determined with immunofluorescence.
RESULTS AND CONCLUSION: ADSCs were successfully extracted from rat fat tissues, and they could also passage infinitely in a considerable large amount. ADSCs strongly expressed CD29 (99.00±0.12)%, CD44 (95.62±0.68)% and low expressed CD45 (0.13±0.12)%. After neurolin induction without serum, induced ADSCs can express nestin; when have been added serum, most of induced cells expressed NF-200 and GFAP. These indicate that ADSCs have robust abilities of duplication. And it has a bionomics orientable differentiated into nerve cells in neural induction circumstance in vitro. ADSCs may be supplied as a source of cells for nervous system injuries and degenerative disease.