Abstract:

BACKGROUND: Autologous transplantation of bone-marrow mononuclear cells for treatment of diabetic peripheral angiopathy has been widely developed at present, which showed good curative effect on ischemic lesions. Case reports also showed that it could improve symptoms of peripheral neuropathy.
OBJECTIVE: To observe the effect of before and after autologous transplantation of bone-marrow mononuclear cells on ischemic lesions and neuropathy index.
METHODS: A total of 83 patients (153 legs) with lower extremity ischemia were treated with autologous transplantation of bone-marrow mononuclear cells. 250-400 mL autologous bone marrow blood of each patient was distilled. Mononuclear cells were extracted by the density gradient method and the number of mononuclear cells were 1.01×10⁸-4.96×10⁹ (average 2.04±0.53×10⁸). Mononuclear cells were injected into both lower extremities and foot intramuscular (1-3 mL/site) for 3cm distance after dilution. Ischemic index and neuropathy index were detected at 3, 6 months after transplantation.
RESULTS AND CONCLUSION: After autologous transplantation of bone-marrow mononuclear cells, the lower limb blood flow and collateral vessels were increased, walking distance was extended, skin temperature was elevated, and the healing of ulcer was promoted. There were significant differences between pre-transplantation and post- transplantation (3 and 6 months after). After treatment for 6 months, the ational symptom of diabetic peripheral nerve was improved, the conduction velocity of motor nerves and sensory nerves was elevated, and there were significant differences between pre- transplantation and post- transplantation (after 6 months).