Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2500-2503.doi: 10.3969/j.issn.1673-8225.2011.14.007

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Effect of human acellular amniotic membrane matrix on synapsin expression of umbilical cord blood mesenchymal stem cells

Hu Wei1, 2, Yang Feng1, Tang You-jia1, Chen Xi-lan1, Yang Bo2, 3, Guan Fang-xia4   

  1. 1Department of Neurosurgery, First People's Hospital of Jiujiang City, Jiujiang  332000, Jiangxi Province, China
    2Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    3Open Laboratory of Key Disciplines, Clinical Medicine of Higher Education of Henan Province, Zhengzhou  450052, Henan Province, China
    4Department of Bioengineering, Zhengzhou University, Zhengzhou  450001, Henan Province, China
  • Received:2010-09-06 Revised:2010-11-18 Online:2011-04-02 Published:2013-11-02
  • Contact: Yang Bo, Chief physician, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Hu Wei☆, Doctor, Associate chief physician, Department of Neurosurgery, First People's Hospital of Jiujiang City, Jiujiang 332000, Jiangxi Province, China; Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China zzdxhuw@163.com

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Abstract:

BACKGROUND: The effect of natural extracellular matrix on neural development of adult stem cell is a hot research spot currently. More studies on neural markers expression at home and abroad, few studies on synapse development.
OBJECTIVE: To investigate the effect of human acellular amniotic membrane matrix on synaptophysin expression of umbilical cord blood mesenchymal stem cells.
METHODS: The 3rd generation of human cord blood mesenchymal stem cells in experimental group was seeded in 24 orifice plate of coating membrane-like matrix cover glass. Time was counted when the inoculation, cells were cultured by basal medium for 4 days. There was no membrane-like matrix, the remaining procedures was the same as the experimental group. Morphologic changes of cells were observed by hematoxylin-eosin staining. Synaptophysin-related protein expression (SYN expression) was detected by immunohistochemical SP method at 2 and 4 days inoculation. SYN expression Ⅰ, Ⅱ, Ⅲ of genetic transcription level was detected by RT-PCR method at 2 and 4 days before and after inoculation.
RESULTS AND CONCLUSION: Cells in control group was fibroblast-like, cells in experimental group extended the processes and connected each other. SYN expression in experimental group was higher compared with control group; there were significant differences (P < 0.01). SYN Ⅰ, SYN Ⅱ, SYN Ⅲ did not express in transcriptional level both before inoculation and at 2, and    4 days in control group. At 2 and 4 days in experimental group, SYN Ⅱ had low expression, SYN Ⅲ had poor expression, and SYN Ⅰ did not express in transcriptional level. The results indicated that acellular amniotic membrane matrix can promote the production and extension of the umbilical cord blood mesenchymal stem cells process, and connected each other, express SYN, and provide favorable conditions for neural differentiation of umbilical cord blood mesenchymal stem cells.

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