Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2491-2494.doi: 10.3969/j.issn.1673-8225.2011.14.005

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Induction and differentiation of bone marrow mesenchymal stem cells into hepatocyte-like cells

Fan Jing-jing1, Li Dong-liang1, He Xiu-hua2, Cheng Bian-qiao1, Ma Ming1, Zhang Zhi-qiang1   

  1. 1Department of Hepatobiliary Medicine, Clinical Medical College of Fuzhou General Hospital, Fujian Medical University, Fuzhou  350025, Fujian Province, China
    2Department of Infectious Disease, First Hospital of Quanzhou, Quanzhou  362000, Fujian Province, China
  • Received:2011-01-10 Revised:2011-02-16 Online:2011-04-02 Published:2013-11-02
  • Contact: Li Dong-liang, Professor, Master’s supervisor, Department of Hepatobiliary Medicine, Clinical Medical College of Fuzhou General Hospital, Fujian Medical University, Fuzhou 350025, Fujian Province, China dongliangli@gmail.com.cn
  • About author:Fan Jing-jing★, Studying for master’s degree, Department of Hepatobiliary Medicine, Clinical Medical College of Fuzhou General Hospital, Fujian Medical University, Fuzhou 350025, Fujian Province, China duimantian@126.com

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Abstract:

BACKGROUND: The research has demonstrated that hepatocyte and fibroblast growth factor and other factors can induce bone marrow mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro, but the optimum environment of induction is still in the exploratory stage.
OBJECTIVE: To observe the differentiation ability of BMSCs into hepatocyte-like cells by hepatocyte growth factor (HGF) and fibroblast growth factor 4 (FGF-4) and other factors.
METHODS: BMSCs of rats were isolated and proliferated by the method of whole bone marrow attachment culture. At the third passage, BMSCs were differentiated into hepatocyte-like cells by HGF, FGF-4, insulin iron-selenium transducin, and hexadecadrol in vitro. The morphological changes of cells were observed at 7, 14, and 21 days. The mRNA expression of albumin (ALB), alpha-fetoprotein (AFP) and cytokeratin 18 (CK 18) was detected by reverse transcription-polymerase chain reaction (RT-PCR). Glycogen was tested by periodic acid-schiff (PAS) at 14 and 21 days after induction, and the expression of ALB was detected by immunofluorescence method.
RESULTS AND CONCLUSION: With the prolonged induction time, BMSCs showed hepatocyte-like, round cells appeared and showed colony growth, liver cell-specific markers gradually emerge and mature. The AFP mRNA expression was appeared at 7 days after induction, and increased significantly at 14 days, then decreased at 21 days. Cells expressed CK18, ALB mRNA and glycogen at 14 days, and increased with the prolonged induction time. The expression of ALB in the cytoplasm is positive at 14 days after induction, and increased significantly at 21 days. BMSCs have the ability of differentiating into hepatocyte-like cells by the method of HGF, FGF-4 and other factors.

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