Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2487-2490.doi: 10.3969/j.issn.1673-8225.2011.14.004

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Expression of Hedgehog signaling molecules in the osteogenesis of rat bone marrow mesenchymal stem cells 

Zhang Xue-ping1, Ma Xiao-li1,2,3, Wang Yun-shan2,3, Shen Hong2, Jia Yan-fei2, Zhao Yun2, Zheng Yan2, Xiao Dong-jie2,3   

  1. 1College of Life Sciences, Shandong Normal University, Jinan  250014, Shandong Province, China
    2Center Laboratory, Jinan Center Hospital Affiliated to Shandong University, Jinan  250013, Shandong Province, China
    3Shandong Research Center of Implantation and Tissue Engineering, Jinan  250013, Shandong Province, China
  • Received:2010-10-19 Revised:2010-11-16 Online:2011-04-02 Published:2013-11-02
  • Contact: Ma Xiao-li, Doctor, Associate professor, Master’s supervisor, College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong Province, China; Center Laboratory, Jinan Center Hospital Affiliated to Shandong University, Jinan 250013, Shandong Province, China; Shandong Research Center of Implantation and Tissue Engineering, Jinan 250013, Shandong Province, China maxl7125@yahoo.com
  • About author:Zhang Xue-ping★, Studying for master’s degree, College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong Province, China xueyx0613163.com
  • Supported by:

    the Medical and Health Development Project of Shandong Province, No. 2005JZ001*; the Key Science and Technology Project of Jinan City, No. 051065*

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Abstract:

BACKGROUND: Hedgehog signaling pathway is an important signal pathway, which regulates the development of variety of tissues and organs in the embryonic stage. It also plays an important role in osteogenesis. However, there are few data about the role of Hedgehog signaling molecules in osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in vitro.
OBJECTIVE: To investigate the expression of Hedgehog signaling molecules during osteogenic differentiation in rat BMSCs in vitro.
METHODS: BMSCs were derived from rat bone marrow, cultured and induced by dexamethasone. Identification of osteoblasts was performed by immunohistochemistry. Level of Sonic hedgehog (SHH) and Indian hedgehog (IHH) during osteogenic differentiation of BMSCs were detected by Western Blot.
RESULTS AND CONCLUSION: BMSCs were successfully obtained. Expression of collagen type I was increased on days 7, 14 and 21 following dexamethasone induction. In the osteogenic differentiation, a higher expression of SHH protein was detected in osteoblast induced group compared with non-induced group at the same time point (P < 0.05). While IHH expression was decreased and significantly lower in osteoblast induced group compared with non-induced group (P < 0.05). The results indicate that Hedgehog signaling molecules involved in the osteogenic differentiation of BMSCs induced by dexamethasone, and SHH and IHH had difference in osteogenic differentiation of BMSCs. 

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