Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (14): 2477-2481.doi: 10.3969/j.issn.1673-8225.2011.14.002

Previous Articles     Next Articles

Effect of dexamethasone on bone marrow mesenchymal stem cells apoptosis in rats

Fan Qie, Zhao Jin-min, Su Wei, Li Xiao-feng   

  1. Department of Traumatic Orthopaedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2010-10-20 Revised:2011-01-14 Online:2011-04-02 Published:2013-11-02
  • Contact: Zhao Jin-min, Doctor, Professor, Department of Traumatic Orthopaedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Fan Qie★, Studying for master’s degree, Attending physician, Department of Traumatic Orthopaedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China fanqie718@yahoo.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30860285/H0605*; the Key Scientific Research Project of Medicine and Health of Guangxi, No. 200636*

PDF

396

Abstract:

BACKGROUND: Several studies have shown that dexamethasone can selectively promote bone marrow mesenchymal stem cells (BMSCs) apoptosis.
OBJECTIVE: To observe the effect of dexamethasone on BMSCs apoptosis and mechanism of action.
METHODS: BMSCs (the first generation) were cultured in vitro; BMSCs of passage 2 cultured were divided into 2 groups: control group and experimental group. Dexamethasone was not added into control group, 10-9, 10-8, and 10-7 mol/L concentration of dexamethasone was added into experimental group, respectively. Apoptosis rate was detected by flow cytometer after 3, 6, 12, 24 hours. MTT assay was used to detect the effect of dexamethasone on proliferation rate of BMSCs. Effects of BMSCs which were deal with by 10-7 mol/L concentration of dexamethasone after 24 hours on Caspase-3 and bcl-2 were measured through RT-PCR.
RESULTS AND CONCLUSION: Compared with the control group, 10-8, 10-7 mol/L concentration of dexamethasone had significant promotion on the apoptosis of BMSCs after 12-24 hours, which was relative to concentration of dexamethasone and time. With the effect of increasing concentration and prolonged time, the apoptosis rate of BMSCs had an increasing trend. The results of MTT showed that 10-8, 10-7 mol/L concentration of dexamethasone had significant effect on the proliferation rate of BMSCs after 24 hours, which was consistent with the above-mentioned detection results of flow cytometer. RT-PCR indicated that the activity of Caspase-3 was increased significantly and activity of bcl-2 was decreased mildly. The results confirmed that dexamethasone promoted apoptosis of BMSCs through extracellular pathway and (or) mitochondria pathway.

CLC Number: