Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8435-8440.doi: 10.3969/j.issn.1673-8225.2010.45.018

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Changes in vascular endothelial growth factor and angiogenesis in dogs with acute myocardial infarction following autologous bone marrow mesenchymal stem cells transplantation

Wang Yong1, Wu Ji-xiong2, Fang Wu-wang1, Lin Bing-lai1, Rui Zu-qin1   

  1. 1 Second Department of Cardiology, Second People’s Hospital of Wuhu, Wuhu  241000, Anhui Province, China;2 Department of Cardiology, First Affiliated Hospital, Anhui Medical University, Hefei  230022, Anhui Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Fang Wu-wang, Doctor, Chief physician, Second Department of Cardiology, Second People’s Hospital of Wuhu, Wuhu 241000, Anhui Province, China fangwuwang@sina.com
  • About author:Wang Yong★, Master, Attending physician, Second Department of Cardiology, Second People’s Hospital of Wuhu, Wuhu 241000, Anhui Province, China hongpingguo2046@sina.com
  • Supported by:

     the Key Scientific Research Program of Natural Science of Department of Education of Anhui Province, No. 2006KJ088A*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can be induced to differentiate into cardiomyocyte-like cells in vivo and in vitro. BMSCs transplantation in local region can promote angiogenesis, prevent ventricle reconstruction, and improve cardiac function, but precise mechanisms remain unclear.
OBJECTIVE: To investigate vascular endothelial growth factor (VEGF) expression and angiogenesis in myocardial infarction zone following BMSCs cardiac transplantation in dogs.
METHODS: Healthy hybrid dogs were randomly assigned to experimental and control groups. All dogs were used to establish models of acute myocardial infarction. At 1 and 2 hours following model induction, BrdU-labeled BMSC suspension was infused into four or five regions of myocardial infarction region in the experimental group, whereas an equal volume of Dulbecco’s modified Eagle’s medium was injected into the dogs of control group. At 4 weeks following transplantation, VEGF concentration in serum was determined using enzyme-linked immunosorbent assay (ELISA). Capillary density was detected using immunohistochemical staining.
RESULTS AND CONCLUSION: On day 1, weeks 1, 2, 3 and 4 following transplantation, VEGF concentrations were increased in the experimental group compared with control group (P < 0.01). Moreover, VEGF concentrations were decreased over time in two groups (P < 0.01). Capillaries per unit area of the experimental group were significantly increased in infarct region compared with control group (P < 0.01). Results showed that after BMSCs transplantation, in myocardial infarct area, the concentration of VEGF is increased and promotes angiogenesis after myocardial infarction.

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