Abstract:

BACKGROUND: Recently, someone presumed that the proliferation of neural precursor cells and neural cells is regulated by Brca-1 gene.
OBJECTIVE: To observe the effect of Brca-1 gene regulation of neural stem cell transplantation on Sprague Dawley rat models of acute ischemic cerebral infarction.
METHODS: Sprague Dawley rat models of acute ischemic cerebral infarction were established according to Longa method. By stereotaxical method, phosphate buffered saline, routinely cultured neural stem cells and 10^-5 mol/L 17 β-estradiol cultured neural stem cells were infused into the lateral ventricle of affected brain of rat models separately. Neurological impairment was evaluated at various time points using animal ethology score. Laser confocal microscopy was utilized to observe Nestin expression in neural stem cells. Cerebral infarction volume on cerebral ischemia reperfusion model rats was evaluated by 2, 3, 5-rihenyltetrazolium chloride (TTC) staining. The brain pathological changes were observed by the optical and electron microscope. Immunohistichemical staining was used to examine the expression of neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP).
RESULTS AND CONCLUSION:  ① The recovery of neural function was better in the 17 β-estradiol group compared with routinely cultured group (P < 0.05). ② Laser confocal microscopy has revealed that fluorescence intensity was strongest at 4 days in the ischemia/reperfusion group and blank group, gradually weak at 7 day. The fluorescence intensity was strongest at 4 days in the 17 β-estradiol group, but the persistent high intensity was long, and the strong intensity was visible at 7 days. ③ The cerebral infarct volume was significantly shortened in the 17 β-estradiol group, and significant difference was detected compared with the ischemia/reperfusion and blank groups (P < 0.05). ④ Neurons were not swollen, with visible nuclei, even chromatin and a majority of normal blood capillary at 7 days following cell transplantation in the 17 β-estradiol group. ⑤ Positive expression of NSE and GFAP was greater in the 17 β-estradiol group compared with routinely cultured group. These suggest that the neuroprotective effect of neural stem cells cultured with estrogen transplantation group is better than routine neural stem cell transplantation group.