Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8426-8430.doi: 10.3969/j.issn.1673-8225.2010.45.016

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Influence of neural stem cell transplantation on the number of p75NGFR positive neurons of the basal forebrain and the ethology of an animal model of Alzheimer's disease with 192-IgG-saporin

Pan Xue-bing1, Long Da-hong2, Luo Xiu-mei2, Tu La-geng1, Pan li1, Wang Gui-ping1   

  1. 1 Department of Anatomical Physiology, School of Nursing, 2 Department of Anatomy, Guangzhou Medical College, Guangzhou  510180, Guangdong Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Long Da-hong, Doctor, Professor, Department of Anatomy, Guangzhou Medical College, Guangzhou 510182, Guangdong Province, China dahonglong88@ yahoo. com.cn
  • About author:Pan Xue-bing★, Master, Physician, Lecturer, Department of Anatomical Physiology, School of Nursing, Guangzhou Medical College, Guangzhou 510180, Guangdong Province, China pxb940135@126.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No.06022674*

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Abstract:

BACKGROUND: Neural stem cells (NSCs) can have a continuous amplification in vitro, have the reproductive activity and plasticity and can survive, migrate, differentiate and integrate into host central nervous system in adult life.
OBJECTIVE: To analyze the effects of NSCs transplantation on the cholinergic p75NGFR positive neurons and behavior of the basal forebrain and behavior of the rat model of Alzheimer’s disease (AD) with lateral ventricle injection of 192-IgG-saporin.
METHODS: A total of 24 Sprague Dawley rats were randomly divided into three groups: normal group, model group and transplantation group. A total of 10 new born Sprague Dawley mouse (< 24 hours) were used for isolation and culture of NSCs. 192-IgG-saporin was injected into the lateral ventricle of Sprague Dawley rats to establish AD animal model. Then, NSCs were transplanted in basal forebrain in the transplantation group. At four weeks, Y-maze test was performed. The changes of morphologic data and number of p75NGFR positive neurons were measured in basal forebrain by using image analysis.
RESULTS AND CONCLUSION: About 1 month after 192-IgG-saporin injection, the number of p75NGFR-positive neurons at the lesion side of medial septal nucleus and vertical diagonal branch was significantly reduced in the model group (P < 0.01). However, the number of p75NGFR-positive neurons at the lesion side of medial septal nucleus and vertical diagonal branch was increased up to 74.85% and 71.66% in the transplantation group, and there were significant differences compared with the model group (P < 0.01). Y-maze test showed the abilities of learning and memory of transplantation group were improved (P < 0.05). The effects of learning and memory may be positively related with the number of p75NGFR neurons of the basal forebrain. These indicated that NSCs transplantation is effective in improving ability of learning and memory and can improve and protect p75NGFR positive neurons of the basal forebrain in 192-IgG-saporin-induced AD models.

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