Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8421-8425.doi: 10.3969/j.issn.1673-8225.2010.45.015

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Effects of bone marrow mesenchymal stem cells modified with brain-derived neurotrophic factor gene on tau protein, beta-amyloid and neuronal ultrastructure in the cerebral cortex and hippocampus of an Alzheimer’s disease rat 

Wei Chang-xiu1, Dai Hong1, Chen Song-lin2   

  1. 1 Department of Neurology, Guizhou People’s Hospital, Guiyang  550002, Guizhou Province, China; 2 Department of Neurology, Huangpu Hospital, Sun Yat-sen University, Guangzhou  510700, Guangdong Province, China
  • Online:2010-11-05 Published:2010-11-05
  • About author:Wei Chang-xiu☆, Doctor, Associate chief physician, Department of Neurology, Guizhou People’s Hospital, Guiyang 550002, Guizhou Province, China weichangxiu@yahoo.com.cn
  • Supported by:

     the Nomarch Foundation Program of Excellent Science and Technology Talents of Guizhou Province, No. (2005)231*

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Abstract:

BACKGROUND: Our previous studies indicted that bone marrow mesenchymal stem cells (BMSCs) modified by brain-derived neurotrophic factor (BDNF) gene could ameliorate the memory function of Alzheimer’s disease (AD) rat.
OBJECTIVE: To observe the effect of BDNF gene-modified BMSCs transplantation on tau protein phosphorylation and beta-amyloid (Aβ) in the cerebral cortex and hippocampus, and neuron ultrastructure in the hippocampal CA1 region of AD model rat.
METHODS: After AD models were established by Aβ stereotactic injection into lateral cerebral ventricle, BDNF modified and unmodified BMSCs were implanted into AD model. One month after transplantation, the expressions of total tau protein and tau hyperphosphrylation in cerebral cortex and hippocampus were detected using Western blotting method. Aβ 40 and Aβ 42 were detected by enzyme-linked immunosorbent assay, and the hippocampal CA1 neuron ultrastructures were observed via transmission electron microscope.
RESULTS AND CONCLUSION: BDNF-modified BMSCs could decrease expression of total tau protein and tau hyperphosphrylation in the cerebral cortex and hippocampus and reduce Aβ 40 and Aβ 42 expression in the cerebral cortex and hippocampus. In AD model group, there were obvious degeneration of organells, such as disruption of rough endoplasmic reticulum, reduction of ribosome, mitochondrial swelling, blurred nuclear membrane. BDNF-modified BMSCs could reduce above-described changes. Its effect was more significant than unmodified BMSCs group.

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