Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8373-8376.doi: 10.3969/j.issn.1673-8225.2010.45.004

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Proliferation and collagen production of bone marrow mesenchymal stem cells cultured in platelet-rich plasma in vitro 

Cao Wen, Yao Guang-hui, Chen Yu-hua, Ji Li-na, Huang Hui, Xia Chang-suo   

  1. Hospital Affiliated to Medical College of Qingdao University, Qingdao  266003, Shandong Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Xia Chang-suo, Associate professor, Doctor, Hospital Affiliated to Medical College of Qingdao University, Qingdao 266003, Shandong Province, China xcs009@163.com
  • About author:Cao Wen, Technician-in-charge, Hospital Affiliated to Medical College of Qingdao University, Qingdao 266003, Shandong Province, China
  • Supported by:

    the Doctor Foundation Program of Hospital Affiliated to Medical College of Qingdao University, No. 2006-41*

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Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) are seed cells that can be obtained greatly for tendon tissue engineering, but how to induce to differentiate into functional tendon-like cells in vitro is a key technology.
OBJECTIVE: To explore the effect of platelet-rich plasma (PRP) on proliferation and collagen production of MSCs cultured in vitro.
METHODS: Rabbit bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured. Autologous PRP was used to intervene BMSCs. We set up PRP-high dose, PRP-moderate dose and PRP-low dose groups and a blank control group. Cells were counted and cell growth curve was drawn. Methyl thiazolyl tetrazolium assay was utilized to analyze BMSCs survival and proliferation ability in each group. Enzyme linked immunosorbent assay was employed to detect collagen. Reverse transcription-polymerase chain reaction was used to determine expression of type Ⅰ collagen gene prior to and following PRP intervention.
RESULTS AND CONCLUSION: BMSCs had high proliferative activity, showing rapid growth and great curve upgrade range in the PRP-high dose, PRP-moderate dose and PRP-low dose groups, which showed significant difference as compared with blank control group (P < 0.05). Long culture time exhibited significant effect. Following culture for a while, its effect presented dose dependence. High dose of PRP had significant proliferative effect. PRP could obviously promote type Ⅰ and Ⅲ collagen synthesis in BMSCs. High dose showed significant effects on stimulating collagen production. These suggest that BMSCs had basic conditions of tissue engineered tendon seed cells. PRP can induce MSC to transform into tendon cells.

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