Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8369-8372.doi: 10.3969/j.issn.1673-8225.2010.45.003

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Expression of alpha-fetoprotein and albumin in bone marrow mesenchymal stem cells induced by hepatic failure rat serum

Wang Ying, Zhao Wen-jing, Ye Dong-xia, Liu Shu-rong   

  1. Jilin Provincial Liver and Gallbladder Diseases Hospital, Changchun  130062, Jilin Province, China
  • Online:2010-11-05 Published:2010-11-05
  • About author:Wang Ying★, Master, Associate chief physician, Jilin Provincial Liver and Gallbladder Diseases Hospital, Changchun 130062, Jilin Province, China xingyuewjz@yahoo.com.cn Zhao Wen-jing, Doctor, Associate chief technician, Jilin Provincial Liver and Gallbladder Diseases Hospital, Changchun 130062, Jilin Province, China Wang Ying and Zhao Wen-jing contributed equally to this article.
  • Supported by:

     the Scientific and Technical Development Project of Changchun City, No. 08SF07*

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Abstract:

BACKGROUND: It was found that hepatocyte growth factor, fibroblast growth factor-4 and epidermal growth factor could induce differentiation of bone marrow mesenchymal stem cells (BMSCs) into hepatocytes. There were no reports on the inducing effect of hepatic failure rat serum on BMSCs.
OBJECTIVE: To study the effects of hepatic failure rat serum on the induced differentiation of BMSCs.
METHODS: BMSCs were isolated from SD rat femur and tibia by density gradient centrifugation and culture in vitro. BMSCs were cultured in DMEM containing the hepatic failure rat serum and conventional culture medium as control. The morphological changes of cells were observed and the expression of alpha-fetoprotein and albumin was measured by immunocytochemistry method on 14 days.
RESULTS AND CONCLUSION: Obvious morphological changes were observed on 14 day, which showed that BMSCs became slightly large and flat cells as epithelial cells. The positive rates of alpha-fetoprotein and albumin were (54.8±7.64)% and (45.9±9.68)% respectively. Cells in the control group did not have morphological changes, no alpha-fetoprotein and albumin expression. The results showed that BMSCs can be induced to express alpha-fetoprotein and albumin by hepatic failure rat serum.

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