Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7543-7546.doi: 10.3969/j.issn.1673-8225.2010.40.030

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Application prospect of induced pluripotent stem cells

Liu Kang1, Yi Qiang-ying1, Song Gui-qin2, Feng Gang1   

  1. 1 Research Institute of Tissue Engineering and Stem Cells, Second Clinical Hospital Affiliated to North Sichuan Medical College, Nanchong  637000, Sichuan Province, China; 2 Department of Medical Biology, North Sichuan Medical College, Nanchong  637007, Sichuan Province, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Feng Gang, Doctor, Professor, Master’s supervisor, Research Institute of Tissue Engineering and Stem Cells, Second Clinical Hospital Affiliated to North Sichuan Medical College, Nanchong 637000, Sichuan Province, China lssmd18@gmail.com
  • About author:Liu Kang★, Master, Research Assistant, Research Institute of Tissue Engineering and Stem Cells, Second Clinical Hospital Affiliated to North Sichuan Medical College, Nanchong 637000, Sichuan Province, China kangliu2009@gmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872614*; the Science and Technology Support Program of Sichuan Province, No. 2008SZ0103*; the Grant for Outstanding Youth Leader of Sichuan Province, No. 2009-05-396*

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Abstract:

BACKGROUND: Differentiated somatic cells can be reprogrammed into induced pluripotent stem cells (iPS) by transduction of defined transcription factors. Undoubtedly, iPS technology is a great breakthrough of stem cell research in recent years. iPS breaks away from restrictions on source material and ethics. iPS technology elicits a great promise for patient-specific cell therapy and regenerative medicine.
OBJECTIVE: To review iPS preparation procedures, limited conditions and mechanisms, collection and application prospect.
METHODS: The first author retrieved PubMed database (www.ncbi.nlm.nih.gov/PubMed) for literatures concerning iPS preparation and production mechanisms published from January 2006 to March 2010. The key words were “induced pluripotent stem cells” in English. Simultaneously, some articles were retrieved by hand. Duplicated articles were excluded. Finally, 34 literatures were included.
RESULTS AND CONCLUSION: The establishment of iPS mainly contains following procedures: ① choice of recombinant factors; ② choice of target cells; ③ introduction of recombinant factors; ④ expression of recombinant factors in target cells; ⑤ production of iPS; ⑥ identification of recombinant cells. DNA methylation, histone modification and methylation and p53 gene expression play important roles in the process of somatic cells reprogrammed into iPS. The study of iPS technique was still in an early phase, but it has a broad application prospect. In particular, the acquisition of patient-specific and disease-specific iPS provides great cell seed source for understanding the onset mechanism of diseases and for evaluating drug safety.

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