Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (23): 4281-4284.doi: 10.3969/j.issn.1673-8225.2010.23.023

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Effects of mobilization and collection of hemopoietic stem cells on normal donors

Chen Xiao-xia, Wang Zhi-ming, Luo Xian-sheng, Xu Dan-dan, Li Xing, Lei Mei-qing   

  1. Department of Hematology, Haikou People’s Hospital Affiliated to Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China
  • Online:2010-06-04 Published:2010-06-04
  • About author:Chen Xiao-xia, Associate chief physician, Department of Hematology, Haikou People’s Hospital Affiliated to Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China chenxia_62@yahoo. com.cn
  • Supported by:

    the Natural Science Foundation of Hainan Province in 2005, No. 80571*

Abstract:

BACKGROUND: Mobilization of granulocyte colony-stimulating factor (G-CSF) is a main method used in healthy voluntary donor. Previous studies have demonstrated that some severe adverse effects occurred in mobilization of G-CSF. This leads to anxiety of the safety of unrelated healthy donors. Will the mobilization of granulocyte colony-stimulating factor have any effect on healthy voluntary donor? It remains unknown whether it is safe?

OBJECTIVE: To investigate the effect of G-CSF on healthy donors.

METHODS: A total of 16 healthy donors were mobilized using G-CSF at a dose of 5-10 μg/kg per day at Haikou People’s Hospital from January 2003 to December 2008. The adverse events were recorded during the process of mobilization and collection. Percentage of CD3, CD19, CD3+4, CD3+8 cells in blood was detected prior to and following mobilization. All donors were followed-up.

RESULTS AND CONCLUSION: No adverse effects were determined during G-CSF mobilization in 10 donors. Three cases affected low-grade fever, headache, muscle and skeleton pain or lumbago. Three cases suffered from fever. Severity was in grade I, which does not cause the termination of the mobilization. Leukocyte number was greater following G-CSF mobilization than prior to mobilization. Leukocyte count returned to pre-mobilization levels at 3 days following stopping mobilization. There was no significant difference in hemoglobin and platelet counts, the percentage of CD3, CD19, CD3+4, CD3+8 cells in blood between pre-mobilization and 72 and 96 hours post-mobilization. Results have indicated that the healthy donors can tolerate the mobilization at a dose of 5-10 μg/kg per day, and G-CSF dose not affect the lymphocyte subsets.

CLC Number: