Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (23): 4285-4289.doi: 10.3969/j.issn.1673-8225.2010.23.024

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Effects of active principle region of Yangxing Tongmai Formula on bone marrow mesenchymal stem cell mobilization and homing in rats with acute myocardial infarction

Li Yong-hua1, 2, Yuan Zhao-kai2, Zheng Jing-hui2, Wang Li-ping2, Jian Wei-xiong2, Huang Xian-ping2   

  1. 1Department of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing   404120, China;
    2Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha   410007, Hunan Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Yuan Zhao-kai, Master, Professor, Doctoral supervisor, Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China yuanzk1520@yahoo.com.cn
  • About author:Li Yong-hua, Studying for doctorate, Attending physician, Department of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing 404120, China; Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China liyonghua200888@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30772696*, 30973717*;
    the Postgraduate Innovation Foundation of Hunan Province in 2009, No. CX2009B168*

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Abstract:

BACKGROUND: During acute infarct myocardium repair process, it is important to seek mobilization agent that can elevate number of peripheral blood bone marrow mesenchymal stem cells (BMSCs) and induce BMSCs homing to damaged myocardium.

OBJECTIVE: To explore the effect of active principle region of Yangxin Tongmai Formula (apr-YTF) on the BMSC mobilization and homing of infarct myocardium in rats.

METHODS: The apr-YTF was primarily composed of ginsenoside, tanshinone ⅡA, total alkaloids, and panaxan, which was supplied by College of Pharmacy, Central South University in accordance with the technique of this group. Among 70 Sprague Dawley rats, 8 rats were considered as normal control group, and the remaining 62 rats was used to establish models of acute myocardial infarction. A total of 32 rat models were randomly assigned to apr-YTF group, Danshen injection group, recombinant human granulocyte colony stimulating factor (rhG-CSF) group and model control group. After 3-hour modeling, corresponding drugs were given in each group for 5 days. Peripheral blood CD34+ cell number was determined using flow cytometry. CD34+ cell number was detected at marginal zone surrounding the infarct myocardium utilizing immunohistochemical staining.

RESULTS AND CONCLUSION: Number of peripheral blood CD34+ cells increased significantly in the rhG-CSF group and apr-YTF group compared with model control group (P < 0.05). CD34+ cell number at marginal zone surrounding the infarct myocardium increased significantly (P < 0.01). Compared with the rhG-CSF group, CD34+ cell number at marginal zone surrounding the infarct myocardium increased significantly in the apr-YTF group (P < 0.01). The apr-YTF could promote BMSCs mobilization into blood, and directly homing to infarct myocardium. Its effects were identical to rhG-CSF. The effects on homing of CD34+ cells were better than rhG-CSF. It was a good agent for BMSCs mobilization.

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