Abstract:

BACKGROUND: In present research of tissue engineered bone construction, seed cells are mainly harvested from bone, periosteum, bone marrow and non-osseous tissue. Present studies mainly focused on bone marrow stromal cells (BMSCs), but the discovery of adipose-derived stromal cells (ADSCs) can replace bone marrow stromal cells.
OBJECTIVE: To study the biological characteristic of ADSCs and BMSCs cultured in vitro and to compare alkaline phosphatase activities following osteogenesis so as to evaluate whether ADSCs could be a better seed cells to replace BMSCs.
METHODS: Adipose tissue and bone marrow from the same body were collected during operation. Adipose tissues were digested with collagenase type Ⅰ to gain ADSCs, and BMSCs were isolated by centrifugation on lymphocyte separation medium. Following in vitro culture and passage, cells were induced to differentiate into osteoblasts. At the second and third weeks after induction, alkaline phosphatase activity in cells was measured for comparison and Von Kossa calcium nodus staining was used to detect osteoblasts.

RESULTS AND CONCLUSION: Adipose tissue and bone marrow were gained from 15 patients and 10 of them brought off a successful experiment. Compared with BMSCs, ADSCs can be cultured more easily and proliferate faster. Their morphological characteristics were similar. Black calcium noduses were seen in extracellular matrix. No significant difference was determined in alkaline phosphatase activity between ADSCs and BMSCs (P > 0.05). The results proved that adipose tissue is abundant. ADSCs can be cultured more easily, and have similar biological characteristics with BMSCs, and they can be easily cultured, proliferate faster, and can be induced to osteoblasts. ADSCs should be a more ideal seed cells than BMSCs