Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (23): 4207-4210.doi: 10.3969/j.issn.1673-8225.2010.23.007

Previous Articles     Next Articles

Immunophenotype determination of rat adipose tissue-derived mesenchymal stem cells at passage 4

Li Dong-fei, Yang Chun, Li Zhen, Dai Jing-xing, Yuan Lin   

  1. Department of Human Anatomy, South Medical University, Guangzhou 510515, Guangdong Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Yuan Lin, Professor, Department of Human Anatomy, South Medical University, Guangzhou 510515, Guangdong Province, China yuanl@fimmu.com
  • About author:Li Dong-fei, Studying for doctorate, Attending physician, Department of Human Anatomy, South Medical University, Guangzhou 510515, Guangdong Province, China mzlidongfei@126. com
  • Supported by:

    the National Basic Research Program (973 Program) of China, No.2007CB512705*;
    the Youth Science Program of National Natural Science Foundation of China, No.30801464*

PDF

449

Abstract:

BACKGROUND: Many studies have confirmed that adipose tissue-derived mesenchymal stem cells (ADMSCs) can be collected using enzyme digestion method. At passage 10, ADMSCs grew well, but their surface marker expression decreases.
OBJECTIVE: To isolate and cultivate rat ADMSCs and detect immunophenotype at passage 4.
METHODS: ADMSCs of six Sprague-Dawley rats from inguinal fat pads were isolated and cultured. Adherent cells were amplified till passage 4. Morphology of ADMSCs was observed under a phase contrast microscope. The immunophenotype markers including CD11b, CD29, CD45, CD49d, CD90 and CD106 of ADMSCs were determined using flow cytometry. 
RESULTS AND CONCLUSION: Rat ADMSCs showed fibroblastic-like morphologic characteristics. The immunophenotype analysis revealed that the rat ADMSCs were positive for CD29 and CD90, but negative for CD11b, CD45, CD49d and CD106. The rat ADMSCs can be obtained through enzyme-based isolation procedures. The results revealed that the immunophenotype characterization of rat ADMSCs is consistent with mesenchymal stem cells.

CLC Number: