Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (1): 53-56.doi: 10.3969/j.issn.1673-8225.2010.01.012

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Differentiation of human bone marrow mesenchymal stem cells-induced nucleus pulposus cells under new-style layer coculture

Li Quan-xiu1, Chen Bo-hua1, Cai Yue-yan2   

  1. 1Department of Spinal Surgery, Hospital Affiliated to Medical College, Qingdao University, Qingdao   266003, Shandong Province, China;
    2Dongming County Hospital of Chinese Medicine, Heze   274500, Shandong Province, China
  • Online:2010-01-04 Published:2010-01-04
  • Contact: Chen Bo-hua, Doctor, Professor, Department of Spinal Surgery, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, Shandong Province, China
  • About author:Li Quan-xiu★, Studying for master’s degree, Attending physician, Department of Spinal Surgery, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, Shandong Province, China sdspine@sina.com

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Abstract:

BACKGROUND: There are some limitations in induced differentiation of bone-marrow-derived mesenchymal stem cells (hBMSCs) and transplantation of nucleus pulposus cells (NPCs). Inhibition of autologous NPCs degeneration is expected to become an effective way for treatment of disc degeneration in the future.

OBJECTIVE: To establish a layered coculture model of hBMSCs and NPCs in vitro, and to observe the effect of hBMSCs on the differentiation of NPCs.

METHODS: The fourth passage of hBMSCs was divided into 2 groups. NPCs in the new-style layer culture group were incubated in Transwell cabin without arm, 0.4-μm membrane well; the proportion to BMSCs was 1: 1. NPCs in the traditional culture group were incubated in Transwell cabin with arm. NPC culture group was set. Each group was incubated for 7 days. Collagen Ⅱ was detected by immunohistochemical method. Aggrecan expression was detected by [35S ]-sulfate uptake.

RESULTS AND CONCLUSION: Compared with NPC culture group, collagen Ⅱ and aggrecan expression of NPCs were upregulated most evidently in new-style culture and traditional culture groups (P < 0.05, P < 0.01). The increased range of new-style culture group was greater than the traditional culture group (P < 0.05). These results suggested that coculture of BMSCs and NPCs can significantly increase NPC proliferation and specific substance expression. The surrounding of new-style culture surpassed the traditional culture.

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