Table of Content

04 January 2010, Volume 14 Issue 1    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Paracrine action of bone marrow mesenchymal stem cells and cell apoptosis following cerebralischemia Li Li-xin, Hao Huai-yong, Tian He-ping, Dai Xue-liang, Hu Wei-xing 2010, 14 (1):  1-5.  doi: 10.3969/j.issn.1673-8225.2010.01.001 Abstract ( 418 )   PDF (470KB) ( 716 )   Save null Related Articles | Metrics

Select

Isolation of glioma stem cells from human glioma cell lines U87 with serum deprivation method Liu Yu-hai, Niu Chao-shi, Li Dong-xue 2010, 14 (1):  6-10.  doi: 10.3969/j.issn.1673-8225.2010.01.002 Abstract ( 524 )   PDF (429KB) ( 666 )   Save BACKGROUND: Cell cycle has proved that about 90% stem cells were in resting state in the G0 phase; therefore, serum deprivation method which did not contain any growth factors and related nutrition additive was beneficial for separating glioma stem cells. OBJECTIVE: To explore the method of serum deprivation to culture glioma cell lines U87, and to isolate and identify the cancer stem cells which are in the U87 cell lines. METHODS: U87 cells were cultured in the medium containing both DMEM and L-glutamine for six days; cancer stem cells would be screened out; then the medium was changed for the neural stem cell culturing medium. The formation of tumor spheres and their differentiation characteristics were observed when they were inoculated onto serum-containing medium. The immunofluorescence staining of cells was employed to identify the surviving cells cultured in serum deprivation medium, tumor spheres and differentiated cells. RESULTS AND CONCLUSION: The serum deprivation method was used to select tumor stem cells successfully which expressed CD133 and could form tumor spheres. The tumor spheres had an ability of multi-differentiations, and the daughter cells expressed glial fibrillary acidic protein and neuron specific enolase. The U87 cell lines exist glioma stem cells which have the capacities of self-renewal and multi-differentiation. Related Articles | Metrics

Select

Epidermal growth factor interferes colony formation of fibroblasts and differentiation into neuron-like cells from non-adherent bone marrow mesenchymal stem cells in mice Wu Yu-xin, Wang Yan, Ben Xiao-ming 2010, 14 (1):  11-14.  doi: 10.3969/j.issn.1673-8225.2010.01.003 Abstract ( 315 )   PDF (421KB) ( 555 )   Save BACKGROUND: Non-adherent mesenchymal stem cells (NA-MSCs) can form colony forming unit of fibroblasts and induce the differentiation into adipocytes, osteoblasts, and chondrocytes. OBJECTIVE: To determine the effect of epithelial growth factor (EGF) on colony formation of fibroblasts and differentiation into neuron-like cells from NA-MSCs. METHODS: Bilateral femur and tibia as well as total MSCs were separated, and repeated-transfer was employed to purify NA-MSCs. The fifth-passage total MSCs and NA-BMCs were induced in a medium containing EGF and basic fibroblast growth factor (b-FGF) for 2 weeks. Colony unit formation of fibroblasts, effect of EGF on colony-forming unit of fibroblasts, and relative protein expression detected by toluidine blue and immunocytochemical staining were observed. RESULTS AND CONCLUSION: Both total MSCs and NA-BMC could generate colony-forming unit of fibroblasts. After treatment of EGF, colony-forming unit of fibroblasts from NA-BMC was increased significantly. Immunocytochemical staining demonstrated that two weeks later both neuro-specific NeuN and NF-200 were observed in total MSCs and NA-BMC; while, toluidine blue staining indicated that neuron-specific Nissl body was observed in some cells. EGF can effectively promote colony-forming unit of fibroblast from NA-BMC, and repeated-transfer NA-BMC can induce differentiation into neuron-like cells. Related Articles | Metrics

Select

Neuronal differentiation of adipose tissue-derived stromal cells Liu Bin, Wu Meng-hai, Dong Jing, Liu Ning, Li Jian-min, Zhang Jin-xia, Li Shi-ying, Wang Rui-min, Chen Gui-liang 2010, 14 (1):  15-18.  doi: 10.3969/j.issn.1673-8225.2010.01.004 Abstract ( 433 )   PDF (434KB) ( 709 )   Save BACKGROUND: Appropriate seed cell is important for transplantation in the treatment of cerebrovascular disease and other central nervous system disease. OBJECTIVE: To investigate the capacity of human adipose tissue-derived stromal cells (ADSCs) to differentiate into neurons. METHODS: The fatty tissue was harvested from removed abdominal unnecessary fat of healthy adult with no communicable disease or endocrine disease. Human ADSCs were isolated from human liposuction tissues and cultured in neural induction medium with GM1. Invert phase-contrast microscopy was used to observe morphology changes of ADSCs. The expression of nestin, neuron specific enolase (NSE) and microtubule-associated protein 2 (MAP2) were identified by immunocytochemistry. RESULTS AND CONCLUSION: The majority of cells displayed typical appearance of neuronal-like cells following induction. Following 1 hours of induction, some cells began to express nestin, and NSE and MAP2-positive cells were observed at 5 hours. ADSCs can differentiate into neurons, and the differentiated neurons have the capacity of expressing nestin, NSE and MAP2. Related Articles | Metrics

Select

Differentiation of rhesus monkey bone marrow mesenchymal stem cells into neuron-like cells induced by sonic hedgehog: Significance of its signal molecule expression changes compared with retinoic acid scheme Song Ge, Zhang Yang, Liu Bing-qian, Zheng Wei-wei, Sun Xue-rong 2010, 14 (1):  19-23.  doi: 10.3969/j.issn.1673-8225.2010.01.005 Abstract ( 319 )   PDF (461KB) ( 433 )   Save BACKGROUND: Present studies have demonstrated that during neural development, differentiation of neural stem cells (NSCs) was affected by various regulatory factors from surrounding microenvironment. Sonic hedgehog (Shh) is a key induction signal during neural fetal development, and can be an effective inductor to regulate differentiation of neural cells. OBJECTIVE: To investigate the signal transduction pathway of SHH for differentiation of rhesus monkey bone marrow mesenchymal stem cells (BMSCs) into of neuron-like cells by sonic hedgehog factor. METHODS: Rhesus monkey BMSCs were isolated and cultured by conventional density gradient centrifugation. BMSCs in the induction group were treated with L-DMEM containing FGF2, B27 and fetal bovine serum for preinduction of 24 hours, and then with DMEM supplemented with 0.5 μmol/L retinoic acid or 400 μmol/L SHH for 8 days. Non-induced cells served as control group. Following labeling with neuron enolase, positive cells were screened by flow cytometry. RT-PCR and Western-blot were used to detect SHH- and retinoic acid-induced cell membrane receptor and intracellular signal protein changes. RESULTS AND CONCLUSION: SHH specific membrane receptor Ptc, retinoic acid specific receptor RARα, signal protein molecule ptch1 and Smad expressed in normal cells. Ptc expression upregulated in SHH-induced cells. High expression lasted for a long time with induction time, which was significantly stronger compared with the retinoic acid and control groups (P < 0.01). Intracellular ptch1 protein molecule expression showed similar tendency as this, but could not induce upregulation of RARα expression. During induction, retinoic acid-stimulated cells did not activate Ptc pathway. Four days following induction, RARα expression upregulated and lasted till 6 days, but there were no significant differences. No significant change in ptch1 expression was determined. SHH- and retinoic acid-induced cell Smad molecule expression upregulated, but no significant difference was determined. Results verified that SHH-induced scheme participated in cell induction and differentiation by persistently activating its specific receptors. However, there was no significant receptor pathway crossing between retinoic acid-induced and SHH-induced schemes. Related Articles | Metrics

Select

Migration and differentiation of neural stem cells derived from a human fetus brain in developmental cerebrospinal fluid Yin Guo-cai, Chen Xin-sheng, Zheng Ai-fang, Wang Zhi-gao, Xie Ling, Wu Gan-lin 2010, 14 (1):  24-27.  doi: 10.3969/j.issn.1673-8225.2010.01.006 Abstract ( 386 )   PDF (454KB) ( 555 )   Save OBJECTIVE: To investigate the effects of human cerebrospinal fluid (CSF) during development phase on migration and differentiation of fetal brain neural stem cells (NSCs). METHODS: Fetal brain cells of gestational age of 16 weeks that were frozen in liquid nitrogen were obtained, resuscitated and incubated in DMEM/F12 medium containing epithium growth factor (EGF), basic fibroblast growth factor (bFGF), B27 and N2. The neurospheres cultured for 14 days were obtained. CSF was absorbed from the subarachnoid cavity and brain ventricle in the embryonic group. CSF was collected by lumbar puncture or ventricular puncture in the child group. The neurospheres cultured for 14 days were transplanted into the pure CSF in an incubator containing 5% CO2 at 37 ℃. Cellular migration and growth of neurospheres in CSF were observed. Effects of CSF on neural cell differentiation were identified by immunofluorescence. RESULTS: Neural stem cells in the form of neurospheres derived from fetal brain were inoculated into the pure CSF, and cell migration were commonly observed besides few of neurospheres in child CSF culture at 6 hours following culture. Surrounding cells of neurospheres extended processes, forming cell cord that became cell webs after extension. Compared with the embryonic group, positive rate of glial fibrillary acidic protein was significantly increased in the children group (P < 0.01), but positive rates of nerve fiber and nestin were significantly decreased (P < 0.01). In addition, galactocerebroside-positive cells were only found in 3 baby CSF cultures. CONCLUSION: There existed significant affections on both migration and differentiation of human neural stem cells when cultured in pure CSF with different developmental phase, suggesting that CSF is one of major niche factors for central neural system development. References | Related Articles | Metrics

Select

Effect of centrifugal force on osteoblastic differentiation of bone marrow stroma cells in vitro Xu Nan-wei, Zhang Yu, Zhou Dong, Sun Rong-bin 2010, 14 (1):  28-32.  doi: 10.3969/j.issn.1673-8225.2010.01.007 Abstract ( 411 )   PDF (420KB) ( 467 )   Save BACKGROUND: Centrifugal force is a contributing factor inducing osteoblastic differentiation from bone marrow stroma cells. OBJECTIVE: To explore whether centrifugal force promote osteoblastic differentiation from rabbit marrow stroma cell seeded on polylactic-co-glycolic acid (PLGA) scaffolds. METHODS: Rabbit bone marrow stroma cells were isolated and cultured by whole bone marrow method, purified by attachment method, and digested by trypsin-EDTA at 80% confluency. The cell concentration was adjusted to 1×109/L. PLGA was cut into pieces, 5 mm×5 mm, soaked in serum-conditioned culture medium for 24 hours. The third passage of bone stroma cell suspension at a density of 300 μL was respectively seeded into the PLGA material. The scaffold/cell compound was placed in centrifuge tube, with cell at the upper layer and cultured in osteoblastic induced medium containing antiscorbic acid, β-sodium glycerophosphate, and dexamethasone respectively under centrifugal force every 12 hours (1 000 r/min for 30 minutes, relative centrifugal force 132 g) and static condition. Alkaline phosphatase activity, osteocalcin content and calcium content as well as observation by light microscopy were used to evaluate osteoblastic differentiation. RESULTS AND CONCLUSION: After 16 days of in vitro culture, the scaffolds of centrifugal force group were coated by multiplayer cells and mineralized matrix, but only a thin layer of cells were observed on the scaffold of control group. The centrifugal force system resulted in a significant decrease in alkaline phosphatase activity at day 2 (P < 0.05) but significant increase at day 4 compared with the static culture condition (P < 0.05). During the whole culture time, osteocalcin secretion remained low in control group. At days 12 and 16, a significant enhancement in osteocalcin secretion was observed for centrifugal force culture compared with static culture conditions (P < 0.05). Moreover, after 16 days of culture a significant increase in calcium deposition was observed in the scaffolds subjected to centrifugal force compared with static culture condition (P < 0.05). Centrifugal force can enhance osteoblastic differentiation and mineralized matrix production of bone marrow stroma cell seeded in PLGA. Related Articles | Metrics

Select

Isolation, culture and osteogenic differentiation of mesenchymal stem cells from human umbilical cord: Ultrastructure of cell membrane observed using atomic force microscope Sun Guo-dong, Wu Shi-xian, Li Zhi-zhong 2010, 14 (1):  33-37.  doi: 10.3969/j.issn.1673-8225.2010.01.008 Abstract ( 324 )   PDF (557KB) ( 590 )   Save BACKGROUND: Mesenchymal stem cells (MSCs) are commonly observed under the scanning electron microscope, transmission electron microscope and inverted microscope. However, above-mentioned observation methods have disadvantages on observing ultrastructure of cell membrane and cytoskeleton. OBJECTIVE: To establish a more effective and appropriate method to isolate, culture and identification of human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro and to study the ultrastructure of osteogenic differentiation with the atomic force microscope. METHODS: The hUCMSCs were isolated from human umbilical cord by digested with collagenase. After serial subcultivation in vitro, the stem cells were passaged, and osteogenic differentiation was determined by alkaline phosphatase calcium-cobalt staining and alizarin red staining. hUCMSCs immunophenotype was measured by Flow cytometry.The membrane surface ultrastructure of osteogenic differentiation was observed by Atomic Force Microscope before and after induction. RESULTS AND CONCLUSION: The isolated hUCMSCs by digested with collagenase was efficient. After seeded 24 hours, the adherent cell showed spindle shape, polygonal shape and fibroblast-cell-like shape and the size of hUCMSCs was homogeneous. Flow cytometry analysis revealed that CD29, CD44, CD105 were highly expressed on the surface of passages 3 cells, but there was negative for CD34, CD45 and HLA-DR. These cells were high positive for alkaline phosphate staining and also showed significant calcium node using alizarin red staining after 4 weeks culture induction of osteogenic differentiation. Under atomic force microscopy, undifferentiated stem cells demonstrated insignificant microtubule protrution in a parallel distribution following analysis of cytoskeleton before and after differentiation. Related Articles | Metrics

Select

Differentiation of bone marrow mesenchymal stem cells into chondrocytes induced by transforming growth factor beta 1 Li Li-yan, Huang Jin-zhong, Du Jiang 2010, 14 (1):  38-41.  doi: 10.3969/j.issn.1673-8225.2010.01.009 Abstract ( 465 )   PDF (474KB) ( 728 )   Save BACKGROUND: Transforming growth factor beta 1 (TGF-β1) is a first selected growth factor in study of articular cartilage tissue engineering. Suitable concentration can stimulate proliferation, division and differentiation of articular chondrocytes.  OBJECTIVE: To establish an special inducing system for differentiation of bone marrow mesenchymal stem cells (BMSCs) into chondrocytes in contained TGF-β1 culture in vivo，and to observe the changes in cell form and phenotype. METHODS: BMSCs were separated and purified from rabbit tibial tubercle using attachment culture method. Surface antigen of the third-generated BMSCs was determined by using flow cytometry. Subsequently，the third-generated BMSCs were induced with TGF-β1-contained special inducing system in 21-day culture, which was compared with human septal cartilage cells of nose following induction. Collagen type Ⅱ was qualitatively determined using immunohistochemical method. RESULTS AND CONCLUSION: Rabbit BMSCs were separated and purified using attachment culture method. The third-generated BMSCs were positive for surface antigen CD44，but negative for surface antigen CD34 and CD45. Cell form was irregular 21 days after induction. Immunohistochemical staining for type II collagen demonstrated positive cells. Results suggested that under culture system containing TGF-β1 BMSCs may directionally differentiate into chondrocytes，and there were not significant differences with normal chondrocytes. Related Articles | Metrics

Select

Growth state and adenovirus infection efficiency of human umbilical cord-derived mesenchymal stem cells in 3 different culture systems Hong Jing-xin, Zhang Qian-zhen, Han Jun-ling, Liu Hui, Liu Jian, Qiu Lu-gui 2010, 14 (1):  42-47.  doi: 10.3969/j.issn.1673-8225.2010.01.010 Abstract ( 305 )   PDF (627KB) ( 578 )   Save BACKGROUND: In vitro culture condition and culture efficiency are different in reported umbilical cord-derived mesenchymal stem cells, and lacked of unified standards. Different derived mesenchymal stem cells have different biological properties. Therefore, it is very necessary to establish a simple and high-performance culture system for umbilical cord-derived mesenchymal stem cells. OBJECTIVE: To observe the growth state of human umbilical cord-derived mesenchymal stem cells in different culture systems in vitro and adenovirus infection efficiency. METHODS: Mesenchymal stem cells were separated from healthy full-termed delivery fetus using collagenase digestion method and purified by adherent culture. These cells were cultured and amplified in DMEM (low glucose), MesenPRO RS™ Medium and STEMPRO® MSC SFM in vitro. The 3-5 passage mesenchymal stem cells were infected by the Ad5-EGFP, Ad5/11-EGFP, Ad5/35-EGFP as multiplicity of infection (MOI)=1, 10, 100. Viral infection and green fluorescence expression were observed at post-infection 24, 56 and 72 hours using inverted fluorescence microscope. RESULTS AND CONCLUSION: The cell morphology in STEMPR® MSC SFM was different from other two culture system and these cells were not easy to adherent after trypsin digestion. Cell doubling time in the MesenPRO RS™ Medium was shorter than other two groups. Mesenchymal stem cells were infected by Ad5/35-EGFP with higher efficiency than other two kinds of adenovirus, but part of cells appeared apoptosis. The infection efficiency of Ad5/11-EGFP was highest. The fluorescence intensity was gradually increased with increased MOI. Related Articles | Metrics

Select

Human umbilical cord blood mesenchymal stem cells differentiate into hepatocypte-like cells: Feasibility of co-culture with human hepatocyte line LO2 in vitro Han Cui-ping, Liu Ji-yong, Gao Lei, Zhang Xiao-hua, Pei Qing-shan, Sun Xin-xin 2010, 14 (1):  48-52.  doi: 10.3969/j.issn.1673-8225.2010.01.011 Abstract ( 278 )   PDF (466KB) ( 619 )   Save BACKGROUND: Some studies have showed that after indirect co-culture, bone marrow mesenchymal stem cells can differentiate into myocardial cells and hepatocypte-like cells. OBJECTIVE: To investigate the possibility of human umbilical cord blood mesenchymal stem cells (UCB-MSCs) to differentiate into hepatocytes by co-culture with human hepatocyte line LO2 in vitro. METHODS: Full-term umbilical cord blood samples were obtained sterilely. The UCB-MSCs were isolated by density gradient centrifugation and directly adherence growth, then passaged with trypsin digestion at 80% cell fusion. By utilizing cell culture plate insets with microporous membrane combined with 6-well plate, the LO2-/UCB-MSCs co-culture system was established. UCB-MSCs were plated into the wells of 6-well plate at a density of 1×107/L. LO2 cells were plated into the cell culture plate insert at a density of 1×105/L. UCB-MSCs were plated in both layers in the control group. Surface markers of adhered cells were detected by flow cytometry. Morphological changes of UCB-MSCs were observed by inverted phase contrast microscopy. mRNA expression was detected by reverse transcriptase PCR. RESULTS AND CONCLUSION: HUCB MSCs expressed CD44 and CD29 strongly, but CD34 and CD45 were expressed negatively. After 5 days, fusiform-shaped cells were reduced in the co-culture group; while, the time passing by, cells shaped irregular round or polygonal were increased, which were similar to hepatocytes. At 4 weeks after culture, UCB-MSCs were still fusiform-shaped in the control group. At day 5 after culture, alpha fetoprotein mRNA expressed positively, but other expressed negatively in the co-culture group; at day 14 after culture, cytokeratin-19 mRNA and albumin mRNA expressions were observed; moreover, with the time passing by, the expression of albumin mRNA was increased, but the expression of alpha fetoprotein-19 mRNA was decreased. Antigenic expressions in the control group were negative. This suggested that UCB-MSCs could differentiate into hepatocypte-like cells by co-culture with human hepatocyte line LO2 in vitro. Related Articles | Metrics

Select

Differentiation of human bone marrow mesenchymal stem cells-induced nucleus pulposus cells under new-style layer coculture Li Quan-xiu, Chen Bo-hua, Cai Yue-yan 2010, 14 (1):  53-56.  doi: 10.3969/j.issn.1673-8225.2010.01.012 Abstract ( 293 )   PDF (398KB) ( 507 )   Save BACKGROUND: There are some limitations in induced differentiation of bone-marrow-derived mesenchymal stem cells (hBMSCs) and transplantation of nucleus pulposus cells (NPCs). Inhibition of autologous NPCs degeneration is expected to become an effective way for treatment of disc degeneration in the future. OBJECTIVE: To establish a layered coculture model of hBMSCs and NPCs in vitro, and to observe the effect of hBMSCs on the differentiation of NPCs. METHODS: The fourth passage of hBMSCs was divided into 2 groups. NPCs in the new-style layer culture group were incubated in Transwell cabin without arm, 0.4-μm membrane well; the proportion to BMSCs was 1: 1. NPCs in the traditional culture group were incubated in Transwell cabin with arm. NPC culture group was set. Each group was incubated for 7 days. Collagen Ⅱ was detected by immunohistochemical method. Aggrecan expression was detected by [35S ]-sulfate uptake. RESULTS AND CONCLUSION: Compared with NPC culture group, collagen Ⅱ and aggrecan expression of NPCs were upregulated most evidently in new-style culture and traditional culture groups (P < 0.05, P < 0.01). The increased range of new-style culture group was greater than the traditional culture group (P < 0.05). These results suggested that coculture of BMSCs and NPCs can significantly increase NPC proliferation and specific substance expression. The surrounding of new-style culture surpassed the traditional culture. Related Articles | Metrics

Select

Expressions of breast cancer resistance protein, cytokeratin-8, and chromogranin-A in human breast carcinoma tissue: Is there a correlation with multi-lineage potential of bone marrow mesenchymal stem cells? Chen Ying-xin, Li Lian-hong, Sun Jie, Wang Bo, Wang Li-xia 2010, 14 (1):  57-62.  doi: 10.3969/j.issn.1673-8225.2010.01.013 Abstract ( 270 )   PDF (508KB) ( 504 )   Save BACKGROUND: Generally speaking, neuroendocrine cells have been not observed in normal breast tissue but found in breast carcinoma tissue which was affected by local microenvironment and hormone level during differentiation of breast epithelial stem cells. OBJECTIVE: By detecting expressions of breast cancer resistance protein (BCRP), cytokeratin-8 (CK8), and chromogranin-A (CgA) in breast carcinoma tissue, to explore the possible mechanism of neuroendocrine cells observed in breast carcinoma tissue during differentiation of multi-lineage potential of bone marrow mesenchymal stem cells. METHODS: BCRP, CK8, and CgA were used as markers for SP stem cells, glandular epithelium differentiation, and neuroendocrine differentiation, respectively. Immunohistochemistry was used to detect the expressions of BCRP, CK8, and CgA in breast tissues of 89 subjects and analyze their correlation. RESULTS AND CONCLUSION: Both BCRP and CK8 expressions were observed in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue. BCRP expression was increased in the breast carcinoma tissue; CK8 expression was decreased with the abnormal differentiation of breast tissue; CgA expression was only detected in breast carcinoma tissue. BCRP expression was significantly correlated with positive CgA expression (P < 0.01), but it was no correlation with positive CK8 expression in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue (P=0.069). The results suggested that neuroendocrine cells were not observed in both normal breast tissues and hyperplastic tissues but in breast carcinoma tissue, which possibly correlated to differentiation of multi-lineage potential of bone marrow mesenchymal stem cells. Related Articles | Metrics

Select

Feasibility of transfection of transforming growth factor-beta 1 into rat dipose-derived mesenchymal stem cells Xu Yu-xia, Deng Zhan-sheng, Luo Wei-min, Miao Shi-jin, Xie Jie, Li Bao-jun 2010, 14 (1):  63-69.  doi: 10.3969/j.issn.1673-8225.2010.01.014 Abstract ( 333 )   PDF (659KB) ( 568 )   Save BACKGROUND: Adipose-derived mesenchymal stem cells (ADSCs) can be induced toward the chondrogenic lineages with chondrogenic medium contained transforming growth factor-β1(TGF-β1). However, it remains concerned about the disadvantage with use of TGF-β1 in vitro, which can induce chemotaxis, activate inflammatory cells, cause local defect and want an ideal matrix to deliver proteins. Therefore, with advanced gene-delivery techniques, TGF-β1 can be long-lasting expressed by transduced stem cells, which is very useful for Chondrogenic Tissue Engineering. OBJECTIVE: To master the method of construction and transfection of eukaryotic expression vector for rat transforming growth factor-β1 and to study the possibility of gene transfection to ADSCs with this vector. METHODS: Recombining DNA techniques were applied to construct recombinant plasmid pcDNA3.1-TGF-β1. And this plasmid was verified by restriction endonuclease mapping and DNA sequencing; Then the plasmid with TGF-β1 gene or not was transfected into ADSCs by use of LipofectamineTM2000. After infection, transduced ADSCs were diluted and cultured with neomycin (G418). Gene transfer efficiency compared on the basis of green fluorescent protein expression was assessed. RESULTS AND CONCLUSION: Digestion of the plasmid with double restriction endo- nuclease XboⅠand Hind Ⅲ showed about two specific electrophoretic strips (1.35 bp and 5.4 kb), and the sequence of the rat TGF-β1 gene in recombinant was concorded with that reported in Genbank. There were 80 percent of the cells which were transduced, and the expressions of mRNA and protein of TGF-β1 in ADSCs were discovered positively. These indicated that the eukaryotic expression vector for rat TGF-β1 (i.e. pcDNA3.1-TGF-β1) , which is able to transfect the ADSCs, can be constructed through genetic recombination. Related Articles | Metrics

Select

Effect of insulin-like growth factor Ⅰ and basic fibroblast growth factor on proliferation and differentiation of human dental papilla mesenchymal cells Xie Jia-min, Tian Wei-dong, Liu Lei 2010, 14 (1):  70-74.  doi: 10.3969/j.issn.1673-8225.2010.01.015 Abstract ( 374 )   PDF (400KB) ( 485 )   Save BACKGROUND: Previous research has indicated that both insulin-like growth factor Ⅰ (IGF-Ⅰ) and basic fibroblast growth factor (bFGF) play an important role in cell proliferation and differentiation. However, the effect on biological characteristics of human dental papilla mesenchymal cells (hDPMCs) still remains unclear. OBJECTIVE: To research the effect of IGF-Ⅰ and bFGF on the proliferation and differentiation of hDPMCs. METHODS: hDPMCs were isolated and cultured in DMEM/F12 culture media containing 1% or 10% fetal bovine serum. The fourth-passaged hDPMCs were incubated in culture media containing 0, 0.1, 1, 10 and 100 μg/L bFGF and 0, 25, 50, 75 and   100 μg/L IGF-Ⅰ(0 μg/L as control group), respectively. At 96 hours after culture, proliferative activity was measured with MTT assay. The corresponding growth factor culture media were used in 10 μg/L bFGF group, 100 μg/L IGF-Ⅰgroup, bFGF + IGF-Ⅰ group, and control group, respectively. At days 1, 3, 5, and 7 after culture, the proliferative activity was detected using MTT assay, and alkaline phosphatase (ALP) activity was measured using modified enzyme kinetics method. RESULTS AND CONCLUSION: At the 0-100 μg/L mass concentration scope, both bFGF and IGF-Ⅰcould accelerate proliferation of hDPMCs, and the proliferation ability of bFGF was superior to that of IGF-Ⅰ; moreover, the combination of bFGF and IGF-Ⅰcaused a synergetic action to proliferation of hDPMCs. The maximal valid concentration of bFGF was 10 μg/L, and the maximal action concentration of IGF-Ⅰwas 100 μg/L. At 0-7 days, the effect of bFGF on the ALP activity of hDPMCs was not obvious, but the effect of IGF-Ⅰon ALP activity of hDPMCs became greater with the time passing; furthermore, the combination of bFGF and IGF-Ⅰcould generate a synergetic action on increasing the ALP activity. Related Articles | Metrics

Select

Basic fibroblast growth factor-induced differences in gene expression of human umbilical cord blood CD34+ and CD133+ stem cells: Gene chip analysis Chen Hui-ping, Li Qian-ru, Zhang Jing, Du Ying,Yang Bo, Li Guo-xi, Hu Xiang, Dong Zi-ming 2010, 14 (1):  75-81.  doi: 10.3969/j.issn.1673-8225.2010.01.016 Abstract ( 330 )   PDF (524KB) ( 596 )   Save BACKGROUND: Further studies are needed to understand the cytobiological character, functional regulation, gene changes and expression difference of CD34+ and CD133+ stem cells induced by basic fibroblast growth factor (bFGF) using gene chip. OBJECTIVE: To compare the differences of gene expression and the response to bFGF of human umbilical cord CD34+ and CD133+ cells, and to explore gene expression changes of bFGF-induced umbilical cord CD34+ and CD133+ hematopotic stem cells/hemapoietic progenitor cells in vitro. METHODS: Human umbilical cord blood CD34+ and CD133+ cells were isolated and purified by MiniMACS immunomagnetic beads selection. The CD34+ and CD133+cells were cultured for 10 to 15 days in DMEM/F12 medium, supplemented with bFGF and B27. Total RNA from these cells was extracted and the genetic level of these cells was performed using Oligo GEArray® chip and GEArray software. Selected rate of CD34+ and CD133+ hematopoietic stem cells was detected using flow cytometry. CD34+ and CD133+ cell morphological changes were measured before and after bFGF induction. The concentration and purity of RNA were determined by agarose gel electrophoresis degeneration. Gene-chip test results were analyzed. RESULTS AND CONCLUSION: ①The 20 samples of cord blood were isolated and purified respectively, CD34+ cell purity (77.52±5.06)%, recovery rate (2.74±1.59)%; CD133+ cell purity (79.16±3.37) % and the recovery (1.12±0.94)%. ②The new selected CD34+ cells were spherical. Following induced by bFGF for 15 days, the majority of cell morphology did not find significant changes, some cells were adherent growth, and protruding and spindle cells were seen. CD133+ cells were spherical, by bFGF in cultured 15 days later, cells were significantly amplified, the round shape changed into an irregular shape, and some cells were adherent growth. ③The total RNA of CD34+ stem cells before and after incubation was respectively 2 236 ng and 1 796 ng. The total RNA of CD133+ stem cells before and after induction was respectively 2 518 ng and 2 191 ng. ④In the detection of 263 genes related to stem cells, two-fold differences of 10 genes in umbilical cord blood CD34+ cells and CD133+ cells, including five kinds of genes expression were higher in the former than the latter, five kinds of genes expression were lower in the former than the latter. After bFGF-induced culture, 32 kinds of gene expression of CD133+ cells were significantly higher than CD34+ cells. Among detected 263 genes, no gene was lower than CD34+ cells. There were only a few gene expression differences of fresh-separated cord blood CD133+ cells and CD34+ cells. The response of CD133+ cells to bFGF was significantly stronger than CD34+ cells, which manifested cell cycle regulation, signal transduction and differentiation, gene expression enhanced. Related Articles | Metrics

Select

Effects of beta-arrestin 2 antigene RNAs on mu-opioid receptor desensitization in C17.2 neural stem cells from mice Gao Feng, Chen Sha-sha, Liu Xi-jiang, Xu Mao, Yang Hui, Tian Yu-ke 2010, 14 (1):  82-85.  doi: 10.3969/j.issn.1673-8225.2010.01.017 Abstract ( 405 )   PDF (441KB) ( 572 )   Save BACKGROUND: Antigene RNAs (agRNAs) could be a useful tool to downregulate beta arrestin 2 (Arrb2) gene expressions, and realize gene knock-out effect in cell levels. OBJECTIVE: To observe the effects of agRNAs on DAMGO-induced mu-opioid receptor (MOR) desensitization in C17.2 cells by using agRNAs complementary to transcription start sites of beta arrestin 2 (Arrb2) to downregulate the gene expression in mouse C17.2 cells.  METHODS: Mouse neural stem cells C17.2 was cultured in Dulbecco’s minimal essential medium containing 10% fetal bovine serum, 10 mg/L penicillin and 10 mg/L ampicillin with 5% CO2 at 37 ℃. The cells were passaged every 5 to 6 days after digestion with 0.25% trypsin when cells were 80% confluent. The expression of MOR on mouse C17.2 cells was detected by RT-PCR and immunocytochemical method. AgRNAs which could silence the expression of Arrb2 was transfected into C17.2 cells with Lipofectamine. The expression of green fluorescent protein gene was observed by fluorescence microscopy 24 hours after transfection. [35S]GTPγS binding was assessed by autoradiography to examine the ability of the MOR to couple to G proteins on stimulation with selective agonist DAMGO. RESULTS AND CONCLUSION: The expression of MOR on C17.2 cells was confirmed by RT-PCR. The receptors were expressed on cell membrane and in plasma determined by immnocytochemistry. The expression of green fluorescent protein gene could be observed in C17.2 cells transfected with plasmid pGPU6/GFP/Arrb9 using fluorescent microsocpe. The results of [35S]GTPγS binding showed that the stimulation ratio in cells with and without DAMGO stimulation or transfected with agRNAs were (113±14)%, (253±17)% and (239±15)% respectively. This indicated that agRNAs could downregulate the expression of beta-arrestin 2 and inhibit the desensitization of MOR. Related Articles | Metrics

Select

Bone marrow mesenchymal stem cell transplantation effect on expression of HCN2 and HCN4 gene following myocardialinfarction Wang Qing-zhi, Bao Wei, Zhou Li, Wang Zhi-yong, Zhang Li 2010, 14 (1):  86-90.  doi: 10.3969/j.issn.1673-8225.2010.01.018 Abstract ( 356 )   PDF (463KB) ( 642 )   Save BACKGROUND: Studies have demonstrated that hyperpolarization-activated andcyclic nucleotide-gated cation channels (HCN) 2 and HCN4 are abnormal expressed in ventricular muscle following heart disease, which is closely correlate to ventricular arrhythmia. Bone marrow stem cells (BMSCs) transplantation can improve damaged cardiac muscle; however, its effect on remodeling of ion channel is unclearly.  OBJECTIVE: To detect the changes of HCN2 and HCN4 expression in left ventricle following BMSCs transplantation. METHODS: Five Sprague-Dawley (SD) rats, aged 3 weeks, were prepared for BMSCs by Percoll method. Additional 30 health, male, SD rats, were randomly divided into DMEM, cell transplantation, sham operation and control groups. At 4 weeks after model preparation, DMEM culture medium was injected into the infarcted area and surroundings with 5 points in the DMEM group. The 3rd generation of cultured BMSCs (200 μL, with 5×106 cells) were injected into rats in the cell transplantation group with the same methods. The mRNA and protein expression of HCN2 and HCN4 gene was detected by RT-PCR and Western blot respectively. RESULTS AND CONCLUSION: In non-infarcted region, the mRNA and protein expression of HCN2 and HCN4 among each groups had no significant difference (P > 0.05). Compared with the control and sham operation groups, mRNA and protein expression of HCN2 and HCN4 in surrounding of the infracted region was elevated in the DMEM group (P < 0.05), which was greater than that of the cell transplantation group (P < 0.05). The mRNA and protein expression of HCN2 and HCN4 was smaller in the center area of infracted region in the DMEM group than that of the control and sham operation groups (P < 0.05), which was similar to the cell transplantation group (P > 0.05). Acute myocardial infarction can increase mRNA and protein expression of HCN2 and HCN4 in infracted region surrounding. BMSCs transplantation may lower the fatality rate of ventricular arrhythmia by reducing HCN2 and HCN4 expression. Related Articles | Metrics

Select

Can bone marrow mesenchymal stem cells be used as PEX gene stable transfection vectors? Gao Ge, Niu Chao-shi, Dong Yong-fei, Zhang Jun, Ding Wan-hai 2010, 14 (1):  91-94.  doi: 10.3969/j.issn.1673-8225.2010.01.019 Abstract ( 373 )   PDF (590KB) ( 491 )   Save BACKGROUND: PEX gene can interfere with the invasion acts of malignant glioma. Bone marrow mesenchymal stem cells (MSCs) are a new type of targeted cell vector on cancer therapy. OBJECTIVE: To construct MSCs stably expressing PEX gene. METHODS: PEX eukaryotic expression vector was constructed by molecular cloning, and identified the recombinant plasmid pcDNA3.1(+)-PEX by restriction endonuclease digestion and sequencing. After transfected with MSCs, the eukaryotic expression vector expression in MSCs was verified by immunocytochemical method. The MSCs stably expressing PEX was established by G418 selection, and then was detected by using reverse transcriptase-polymerase chain reaction. RESULTS AND CONCLUSION: The MSCs stably expressing PEX gene is successfully established, in which PEX gene is highly expressed at both gene level and protein level. Related Articles | Metrics

Select

Establishment of human acute myeloblastic Leukemia M2 type nude mouse model Liu Yuan, Dong Rui-hong, Li Yu-hua, Wang Ya-ya, Wu Jing, Huang Rui, Deng Lan, Song Zhao-yang, Lu Zhi-gang, Hu Hai-yan 2010, 14 (1):  95-98.  doi: 10.3969/j.issn.1673-8225.2010.01.020 Abstract ( 560 )   PDF (377KB) ( 607 )   Save BACKGROUND: It is easy to established human solid tumor nude mouse model, but for leukemia which is difficult. We inhibited immune system further by radioactive ray or CTX, to decrease cost and increase the stability. OBJECTIVE: To establish a human acute myeloblastic leukemia M2 Kasumi-1 models containing AML/ETO positive genes in BALB/c nude mouse.  METHODS: Nude mice were randomly divided into three groups: CTX group was injected CTX 2 mg/day in abdominal cavity for two days, and injected 8×105/mouse Kasumi-1 cells in caudal vein next day; irradiation group was exposed to total body irradiation, and injected 8×105/mouse Kasumi-1 cells in caudal vein that day; untreated group was inoculated with 8×105/mouse Kasumi-1 cells by caudal vein injection. Three additional mice were considered as the normal control group. The blood smearing and bone morrow slides were detected, immunity type of BMC was detected using flow cytometry, loading of leukemic cellular tumor was detected using RT-PCR, and positive ratio of AML/ETO fusion gene was detected using FISH method. RESULTS AND CONCLUSION: After inoculated into untreated nude mice by caudal vein injection for 14 days, the ratio of leukemia cell in blood smearing was 3.5%, and over 40% in bone marrow slides, which was equal to the results of FISH and FCM. The increasing of tumor loading was time-dependent. For irradiation group and CTX treated group, the tumor loading was higher that untreated group, and the cells also survived more than 60 days. AML/ETO band was observed by RT-PCR in all experimental groups, for normal mice it was negative. The results indicated that the systemic disseminated leukemia model was established successfully by caudal vein injection 8×105/mouse Kasumi-1 cells in the three experimental groups Related Articles | Metrics

Select

Killing effect of costimulated activation of peripheral blood mononuclear cells with CD80 and CD28/CpG ODN on gastric cancerous cells MKN45 Fang Jun, Li Yong-yan, Guo Shu-jun 2010, 14 (1):  99-102.  doi: 10.3969/j.issn.1673-8225.2010.01.021 Abstract ( 391 )   PDF (386KB) ( 617 )   Save BACKGROUND: Maintenance and activation of cascade reaction influence T cell proliferation or transformation into nonreactive state even apoptosis. B7 binding to CD28 effectively activates T cells in combination with T cell receptor pathway, and enhances T cell proliferative activity. OBJECTIVE: To investigate the costimulated activation of peripheral blood mononuclear cells (PBMC) with CD28 and CpG containing oligodeoxynucleotides (CpGODN) MoAb combined with CD80, and its killing effect on human gastric cancerous cell line MKN45 in vitro. METHODS: PBMCs were isolated by Ficoll density gradient centrifugation method, and cocultured with interleukin-2, CD28 and CpGODN MoAb for 1-5 days. MKN45 cells were divided into 4 culture conditions: CD28/CpGODN, CD80 plus CD28/CpG ODN, CD80 alone, and blank control．The killing efficiency was measured by MTT method．The ultramicrostructure of cells was observed by electron microscope. Apoptosis was verified by a flow cytometery. RESULTS AND CONCLUSION: CD80 alone did not display killing effect on MKN45 cells. By MTT method, combination of costimulated activation of PBMC with CD28/CpGODN and CD80 showed enhanced killing effect compared with single therapy  (P < 0.05), and the ratio of effector cell and target cell at 15: 1 resulted in half killing efficiency. Electron microscope and flow cytometery verified necrotic or apoptotic cells after 24 hours exposure to costimulated activation. Compared with blank control group, CD80 alone elevated the apoptosis rate of MKN45 cells (P < 0.01). Results from the present study show that CD80 can elevate the killing effect of costimulated activation of PBMC with CD28/CpGODN on MKN45 cells in vitro. Related Articles | Metrics

Select

Astragalus membranaceus injection promotes hepatocytic differentiation of umbilical cord blood stem cell: Enhancement of cell transplantation for the treatment of liver failure Zhang Yong-hong, Zeng Yan, Tang Xiao-peng 2010, 14 (1):  103-107.  doi: 10.3969/j.issn.1673-8225.2010.01.022 Abstract ( 300 )   PDF (424KB) ( 598 )   Save BACKGROUND: Studies of umbilical cord blood stem cells transplantation for liver function failure have demonstrated that Astragalus membranaceus preparation can stimulate hemopoietic stem/progenitor cell proliferation. OBJECTIVE: To explore the effect of Astragalus membranaceus injection on the differentiation of umbilical cord blood stem cell into hepatocyte in vitro and in vivo for the treatment of liver failure. METHODS: The third and fourth passage of human umbilical cord blood stem cells (HUCBSCs) were collected. In drug screening test, there were 4 groups: cells were separately cultured with 0, 40, 200, and 400 mg/L Astragalus membranaceus injection to screen the appropriate for cell growth. In cell differentiation test, there were 2 groups: HUCBSCs were respectively cultured with hepatocyte growth factor (HGF, 10 μg/L), and HGF (10 μg/L) plus 200 mg/L Astragalus membranaceus injection. D-aminogalactose was intraperitoneally injected to establish a model of acute liver failure. Surviving model rats (48 hours) were randomly divided into six groups: model control, Astragalus membranaceus injection, rat peripheral blood mononuclear cells, combination, combination+Cytoxan, and combination+dexamethasone groups. The alpha fetoprotein mRNA and albumin mRNA expression was determined by RT-PCR, and liver function indexes were observed. RESULTS AND CONCLUSION: Different mass concentration of Astragalus membranaceus injection displayed varied influence on HUCBSC proliferation: 200 mg/L was the best for HUCBSC proliferation. Compared with HUCBSC cultured with HGF alone, the number of albumen-positive cells in HUCBSCs cultured with 200 mg/L Astragalus membranaceus injection and HGF was greater (P < 0.05). Moreover, the expression of albumen mRNA in combination, combination+Cytoxan, and combination+dexamethasone groups was greater than rat peripheral blood mononuclear cells group, while alpha fetoprotein mRNA expression was only greater than rat peripheral blood mononuclear cells group in early stage. At 7 days of treatment, the values of alanine aminotransferase, aspartate amino transferase and total bilirubin were significantly greater in combination, combination+Cytoxan, and combination+dexamethasone groups compared with model control, Astragalus membranaceus alone and rat peripheral blood mononuclear cells groups (P < 0.05), but no differences were observed among model control, Astragalus membranaceus alone and rat peripheral blood mononuclear cells groups (P > 0.05). Results indicate that Astragalus membranaceus injection at 200 mg/L can promote the proliferation and differentiation of HUCBSCs into hepatocyte in vitro and in vivo, ameliorate liver function and improve treatment effect of HUCBSC transplantation for liver failure. Related Articles | Metrics

Select

Myocardial glucose metabolism and perfusion following coronary artery bypass grafting and bone marrow CD34+ cell transplantation: Dual-isotope imaging evaluation Zhang Guo-xu, Hao Shan-hu, Wang Zhi-guo, Zhang Tong, Wang Hui-shan, Chen Xian-ying 2010, 14 (1):  108-111.  doi: 10.3969/j.issn.1673-8225.2010.01.023 Abstract ( 360 )   PDF (415KB) ( 526 )   Save BACKGROUND: For patients with myocardial infarction occupied most of the heart, the effect of coronary artery bridge is not obvious. Currently, myocardial and vascular regeneration by stem cells has become a focus of ischemic cardiovascular disease. Myocardial survival directly correlates with improvement of blood perfusion following stem cell transplantation. OBJECTIVE: To investigate the feasibility of 18F-FDG and 99Tcm-MIBI single photon emission computed tomography imaging in assessing myocardial glucose metabolism and perfusion with old myocardial infarction after coronary artery bypass grafting (CABG) and CD34+ stem cell transplanting. METHODS: Bone marrow was extracted from the anterior superior iliac spine 1 day before surgery. Mononuclear cells were isolated by Ficoll density gradient centrifugation. CD34+ cells were isolated and purified by immunomagnetic bead system. Coronary artery pathological changes were examined under general anesthesia. The end-to-side anastomosis of graft vessel and coronary artery was performed. 1×1011/L CD34+ cell suspension was extracted, and injected into the surrounding and center of the infarct (blood flow/metabolism matching depletion) at 6 points, with 0.2 mL in each point. According to preoperative perfusion/metabolism imaging, myocardium segments were divided into two groups: match group: blood perfusion and metabolism images were sparse or normal, i.e. infarction or normal myocardium; mismatch group: blood perfusion image displayed depletion, but metabolism images were normal or radially distributed, i.e. surviving myocardium. 18F-FDG and 99Tcm-MIBI dual-isotopic imaging were performed before and 4 months after CABG. Circumferential count profiles from 18F-FDG and 99Tcm-MIBI short axis slices were generated to assess myocardial blood perfusion and glucose metabolism. RESULTS AND CONCLUSION: The 31 patients were divided into 279 segments, and 145 segments were in myocardial perfusion–metabolism mismatch (MM). 99Tcm–MIBI and 18F-FDG uptake fraction was significantly increased 4 months before operation (P < 0.01); match group without transplanting had 81 segments, and the 99Tcm–MIBI and 18F-FDG uptake fraction remained unchanged after operation (P > 0.05). Match group undergoing transplanting had 54 segments, and their 99Tcm–MIBI and 18F-FDG uptake fraction increased remarkably 4 months after operation (P < 0.01). CABG can improve the function of survival myocardial segments, but it is helpless to infraction myocardium. The autologous CD34+ stem cell transplantation can improve myocardial blood perfusion and glucose metabolism of the distributions of infract myocardium.   Related Articles | Metrics

Select

Electrophysiological changes and hindlimb motor function of a model rat with spinal cord injury following olfactory ensheathing cell transplantation Wang Guang-zhi, Liu Ming-na 2010, 14 (1):  112-115.  doi: 10.3969/j.issn.1673-8225.2010.01.024 Abstract ( 331 )   PDF (396KB) ( 530 )   Save BACKGROUND: Previous research has proved that olfactory ensheathing cells can promote neuronal survival and axonal regeneration. OBJECTIVE: To investigate the effect of olfactory ensheathing cell transplantation on the treatment of spinal cord injury of rats. METHODS: A total of 40 healthy adult female SD rats were randomly divided into control and cell transplantation groups, with 20 rats for each group. Ten additional SD rats were used for separation and culture of olfactory ensheathing cells. Spinal cord injury was induced in both control and cell transplantation groups. 2-cm bilateral 8th-10th intercostal nerves were crossly implanted into spinal cord defect region, i.e., proximal white matter and distal gray matter, distal white matter and proximal gray matter. Olfactory ensheathing cells at density of 2×106 were locally injected into cell transplantation group, while an equal amount saline was locally injected into control group. Somatosensory evoked potential and motion evoked potential were detected to observe neuro-electrophsiological recovery; BBB was used to evaluate hindlimb motor function; BDA anterograde tracer was used to observe motor conduction recovery. RESULTS AND CONCLUSION: Latency and amplitude of somatosensory evoked potential and motion evoked potential in the cell transplantation group were significantly greater than control group (P < 0.01). BBB scores of cell transplantation group were significantly greater than control group (P < 0.01). BDA-positive nerve fibers in the cell transplantation group were significantly more than control group (P < 0.01). Local injection of olfactory ensheathing cells can improve neuro-electrophysiological changes and promote hindlimb motor functional recovery following spinal cord injury. Related Articles | Metrics

Select

Heart function changes following transplantation of autologous bone marrow mononuclear cells in a canine model of heart failure induced by rapid ventricular pacing: Pathological image analysis of collagen fiber Li Hai-rong, Xu Ai-guo, Zhang Yun-qiang, Qi Xiang-qian 2010, 14 (1):  116-120.  doi: 10.3969/j.issn.1673-8225.2010.01.025 Abstract ( 247 )   PDF (478KB) ( 702 )   Save BACKGROUND: Stem cell regeneration can repair injured myocardium. However, bone marrow mononuclear cells (BM-MNCs) transplantation for non-ischemic heart failure remains poorly understood. OBJECTIVE: To investigate effect of transplantation of autologous BM-MNCs on cardiac function in canine model of heart failure by rapid ventricular pacing. METHODS: Implantation and model control groups were subjected to model establishment of heart failure by rapid pacing of apex of right ventricle, and respectively injected with CM-DiI-labeled BM-MNCs and normal saline into myocardium. After 4 weeks, all dogs were sacrificed, and specimens of myocardium were collected from the apex, anterior wall and interventricular septum. All specimens were labeled by FITC. Myocardial fibrosis conditions of implanted cells were observed, collagen volume fraction was determined, and hemodynamic indexes were measured. RESULTS AND CONCLUSION:BM-MNCs labeled by CM-DiI and FITC were observed in the transplantation group showing yellow fluorescence, while in the control group FITC-labeled green fluorescence was seen. HE and Masson staining showed that inflammatory cell infiltration in interstitial matrix, displaying interstitial fibrosis and myocardial fibrosis in model control group, but no obvious inflammatory cell infiltration or myocardial fibrosis was observed in the transplantation group, indicating a success model establishment of heart failure by rapid ventricular pacing. Compared with model control group, the collagen volume fraction decreased significantly (P < 0.05), ejection fracture remarkably increased (P < 0.05), but left ventricular end-diastolic and end-systolic diameter remained unchanged in the transplantation group (P > 0.05). Autologous BM-MNCs in canine model of heart failure show myocardium-like cells differentiation, and improve heart function, which possibly associate with the ability of inhibiting the myocardial fibrosis. Related Articles | Metrics

Select

Autologous peripheral blood stem cells transplantation for the treatment of dilated cardiomyopathy: A 24-month follow-up in 38 cases Wu Zhao-hui, Yuan Ming-yuan, Li Hai-miao, Qiu Jing-jing, Lao Han-zhu, Wu Xiang-yuan, Lin Jin-xiang 2010, 14 (1):  121-125.  doi: 10.3969/j.issn.1673-8225.2010.01.026 Abstract ( 319 )   PDF (431KB) ( 571 )   Save OBJECTIVE: To identify long-term outcomes and safety of transplantation of autologous peripheral blood stem cells (PBSC) for treating dilated cardiomyopathy. METHODS: A total of 38 cases with dilated cardiomyopathy received treatment at the Department of Cardiology, Guangdong General Hospital of Chinese People’s Armed Police Forces, were selected, including 26 males and 12 females, aged 42-72 years, mean aged 56 years. Based on given standard therapy, 38 patients divided randomly into the transplantation group (n=20) and the control group (n=18). Patients in the transplantation group were received recombinant human granulocyte colony-stimulating factor (rhG-CSF) 300 ug/d once per day for 5 days to mobilize stem cells. At day 6, PBSC were collected with blood-cells separator and were transplanted through intracoroary way. The routine medication was performed in the control group. Blood routine test, hepatic function, renal function, glucose, triglyeride (TG), cholesterol, low density cholesterol (C-LDL), high density cholest- erol (C-HDL), uric acid (UA), creatine kinase (CK), isoenzyme of creatine kinase (CK-MB) and high sensitive C-reactive protein (hsCRP) were measured before and at months 6 and 12 after transplantation. All patients also received ultrasonic echocardiography, ECG Holter monitor and six-minute-walk test before and at 12 and 24 months after the procedure. Survival rate and incidence rate of heart incidents were compared. The study end-point was death from any cause. RESULTS: All patients received a 12-24 month follow-up with mean (18±6) months. One patient in the transplantation group received mitral valve replacement. One patient of the transplantation group and 2 of the control group died due to refractory heart failure. The blood routine test and biochemical indicators of the transplantation group had no significant differences among 6 months and 12 months after transplantation compared with control and pre-transplantation (P > 0.05). Six-minute-walking distance in the transplantation group significantly increased at 12 months after transplantation than pre-transplantation level, which was also higher than that of control patients (P < 0.05). The left ventricular ejection fraction (LVEF) was increased (P < 0.01). The left ventricular diastolic diameter (LVDd) decreased significantly in the transplantation group (P < 0.01). In the control group, improvement in LVEF and LVDd were observed, but there was no significant difference (P > 0.05). After 24 months of follow-up, the above-mentioned indexes had not improved in the transplantation group without significant differences. No malignant arrhythmias and severe side effects could be observed around transplantation and during 24 months follow-up. Survival was similar between the two groups during 24 months follow. CONCLUSION: Transplantation of mobilized autologous PBSC might be a safe and effective method for the treatment of dilated cardiomyopathy, which may improve the ventricular systolic function in a short-term, however, the long-term effects still uncertain Related Articles | Metrics

Select

Peripheral blood stem cell transplantation for treating nervous system disease: Possibility, feasibility, and confidence Zhou Shao-yu, Zhang Cheng-wen, Tang Bi-jia, Zeng Xian-zhi 2010, 14 (1):  142-146.  doi: 10.3969/j.issn.1673-8225.2010.01.030 Abstract ( 384 )   PDF (537KB) ( 681 )   Save BACKGROUND: Peripheral blood stem cells (PBSCs) can be enhanced and differentiates into all kinds of neural cells and produce several kinds of neural growth factors in vitro, and have been used to treat several kinds of neural diseases and they showed satisfactory outcomes. It is now a research focus to optimize the culture conditions to induce the stem cell to differentiate into neural cells. On the other hand, it also becomes a focus of its amplification and differentiation.   OBJECTIVE: To make a review on researches on treating brain lesion, genetic defection or degenerating diseases using PBSCs during recent years. METHODS: Researching Pubmed database(1999-01/2009-09), using “peripheral blood stem cell, neural, repair” as research words and these Chinese words in CNKI database(1999-01/2009-09). RESULTS AND CONCLUSION: 221 articles were collected altogether including 41 Chinese articles and 180 English articles. Totally 27 articles were adapted altogether after rejecting published early, repeat and similar articles. PBSCs can be enhanced and differentiates into all kinds of neural cells in vitro, and delivered into central neural system and improve the function of lesion neural area. It is not fully understood the differentiation mechanism of peripheral blood stem cells, but numerous studies have shown that surface marker antigen of peripheral blood stem cells is closely associated with its biological characteristics. There are many studies addressing surface marker of peripheral blood stem cells, but researchers cannot identify a reliable marker that can directly label peripheral blood stem cells. Function of CD34 remains unclear, so the principle of CD34 as a marker of hematopoietic stem cells receives challenge. It is necessary to do further researches on issues on how to enhance survival rate of PBSCs in vivo and the tendency to differentiate into neural cells. Related Articles | Metrics

Select

Influence of adult bone marrow mesenchymal stem cells on solid organ transplantation: Significant candidate cells during tolerance induction Liu Kui-li, Shi Bing-yi 2010, 14 (1):  147-151.  doi: 10.3969/j.issn.1673-8225.2010.01.031 Abstract ( 283 )   PDF (476KB) ( 489 )   Save BACKGROUND: Mesenchymal stem cells (MSCs) from adult bone marrow can alter alloimmune response in vitro and vivo. Their potentiality is great in solid organ transplantation. OBJECTIVE: To develop MSC antirejection therapy and identify behind mechanishm of MSCs immunomodulation ability. METHODS: We searched Pubmed (1994-Mar.2009) with the key words of “mesenchymal stem cells, solid organ transplantation, tolerance, immunosuppression, animal model”. RESULTS AND CONCLUSION: Totally 262 English articles about influence and mechanism of action of adult bone marrow mesenchymal stem cells on solid organ transplantation were collected. The 49 suitable articles were included without earlier publication time, repeated and analogous study. Human mesenchymal stem cells did not express MHC2 Ⅱ antigen and T cell costimulatory molecules B7. Coculture with allogenic T lymphocytes could not induce T cell proliferation, but inhibited mixed lymphocyte reaction and mitogenstimulated T cell proliferation. Inhibitory effects of mesenchymal stem cells on T cell proliferation were not limited by major histocompatibility complex. Mesenchymal stem cells no matter from donors or recipients had similar immunoloregulation effects. Allogene mesenchymal stem cells could cause immunereaction in vivo, no complete immune privilege. The in vivo effects of mesenchymal stem cells will strongly depend on their localization and migration pattern after injection. Therefore, MSCs are interesting candidate cells for tolerance induction in clinical organ transplantation. Related Articles | Metrics

Select

Multiple myeloma stem cells: Effect and significance of surface markers and signal transduction pathways Yan Yan-hong, Li Hui-min 2010, 14 (1):  152-156.  doi: 10.3969/j.issn.1673-8225.2010.01.032 Abstract ( 411 )   PDF (461KB) ( 579 )   Save BACKGROUND: Cancer stem cell is a minority population of cancer cells in multiple myeloma possessing the properties of stem cells: self-renewal, multi-directional differentiation and long-term proliferation, which mediating disease initiation, relapse, progression and drug resistance. OBJECTIVE: To review characteristics of biology, phenotypic analyses, sorting and identification in clonogenic myeloma cells, the signaling pathways with in myeloma stem cells and the target therapy related. METHODS: Application of computer search Medline database (1999-01/2009-04), using “multiple myeloma stem cells, heterogeneity, phenotypic analysis, signaling pathways, target therapy” as key words; application of computer search CNKI database (1999-01/2009-04) and CBM Database (1999-01/2009-04) using “multiple myeloma stem cells, heterogeneity, stem cell separation and identification, signal transduction, targeted therapy as key words”. RESULTS AND CONCLUSION: We collected for 126 literatures on the multiple myeloma stem cell-related, which include 30 Chinese articles and 96 English articles, excluding published earlier, repeated, and similar studies into 30 sub-standard literatures. Now widely recognized that multiple myeloma stem cells may be derived from normal stem cells, the accumulation of mutations and by gene mutation in regaining self-renewal capacity of progenitor cells or fully differentiated mature cells. Circulating clonotypic memory B-cell populations have self-renewal and multi-directional differentiation potential, which represent the cancer stem cells in multiple myeloma. The exact phenotype of multiple myeloma cancer stem cells has not been definitively established and researched remains. At present, both the side population cells and aldehyde dehydrogenase (ALDH) activity assays were mainly capable of isolating multiple myeloma cancer stem cells. Which to possess self-renewal ability by Hedgehog ,Wnt and Notch signaling pathways. When these signal transduction pathways appear abnormal, leading to the occurrence of the tumor and tumor cell growth in non-controlled. Against the cancer stem cell targeted therapy is a new and important direction of selective therapeutic strategies. Cancer stem cell specific surface markers and signal transduction pathways can be used as anti-cancer stem cells to control tumor development targets. Related Articles | Metrics

Select

Characteristics and application of epidermal stem cells: How far is it from basic research to clinical application? Li Jun-nan, Liu Liu 2010, 14 (1):  157-160.  doi: 10.3969/j.issn.1673-8225.2010.01.033 Abstract ( 371 )   PDF (466KB) ( 525 )   Save BACKGROUND: Epidermal stem cell as a specific stem cell in skin tissue, not only plays an important role in maintaining the metabolism of skin, but also closely related to wound repair, is the basis of occurrence of skin and its appendages. OBJECTIVE: To summarize the research status on features and application of epidermal stem cells in recent years. METHODS: A computer-based online search was conducted in PubMed Database and CNKI Database with the key words of “epidermal stem cell, basic study, gene therapy” from June to August, 2009. RESULTS AND CONCLUSION: 532 articles were received in the first round, including 283 in Chinese and 249 in English. Articles which were unrelated articles (n=127), repeated research (n=158), and Meta analysis (n=25) were excluded. A total of 31 articles were reserved for review. Epidermal stem cell had the typical characteristics of long periodicity in vivo, self- renew capacity and easy to adherent to skin basement membrane. They had constant position in tissues which were located in basal layer and hair follicle bulge with rich blood supply in epidermis. The proliferation and differentiation of epidermal stem cells were affected by external and internal factors. The former meant microenvironment which stem cell existed in, that was called “Niche” of stem cell; the latter included integrin-mitogen-activated protein kinase pathway, Wnt signaling pathway, c-Myc proto-oncogene, and Notch signaling pathway. Currently, common marker of epidermal stem cells includes integrin, keratin, p63, p75, and epidermal cell-surface transferrin receptor CD71, etc. Epidermal stem cells can be explored in tissue engineering and wound repair, gene therapy, epidermal tumors, etc. Related Articles | Metrics

Select

Application and research progress in stem cells therapy for sensorineural deafness Qin He, Yang Shi-ming, Zhai Suo-qiang 2010, 14 (1):  161-165.  doi: 10.3969/j.issn.1673-8225.2010.01.034 Abstract ( 412 )   PDF (436KB) ( 685 )   Save BACKGROUND: The sensorineural deafness occurs as a result of loss of inner ear hair cells in the cochlea or of their primary afferent the spiral ganglion neurons. Stem cells to restore hearing following inner ear cell death has become a focus in recent years. OBJECTIVE: To summarize research progress in stem cells differentiating into inner ear cells in vitro and in vivo and to review the achievement in stem cells replacing inner ear cells in treating sensorineural deafness. METHODS: With “inner ear, stem cells” as key words, a computer-based online search of Pubmed and CNKI was performed for articles published from January 2000 to August 2009. RESULTS AND CONCLUSION: A total of 170 articles were collected, and experimental studies and review articles on stem cells in sensorineural deafness were included, while repetitive articles were excluded. Finally, 32 articles were summarized and analyzed. Different types of stem cells have the capacity to differentiate into inner ear cells. They can differentiate into neural cell types. Stem cells can live and migrate, differentiating into cell types of the sites of injury. It provides a therapy strategy to restore hearing following sensorineural deafness by he capacity of stem cells differentiating into inner ear cells. However, it remains further investigation how to function following cell differentiation and how to form the appropriate neural pathways by stem cell transplantation in sensorineural deafness. Related Articles | Metrics

Select

Role of adipose-derived mesenchymal stem cells in bone tissue engineering Jia Jing-han, Zhang Zhi, Wang Xiang-peng, Zou Dong-qing 2010, 14 (1):  166-170.  doi: 10.3969/j.issn.1673-8225.2010.01.035 Abstract ( 384 )   PDF (491KB) ( 526 )   Save BACKGROUND: At present, bone defects usually repaired by autologous bone, allogenic bone, synthetic bone substitutes and other methods, which received poor clinical results. Preliminary studies have shown that adipose-derived mesenchymal stem cells (ADSCs) possess strong proliferation ability and differentiation potential, and can be induced differentiate into bone. OBJECTIVE: To analyze the application of ADSCs in bone tissue engineering, and to identify whether ADSCs can be used as seed cells in bone tissue engineering. METHODS: The databases of PubMed (1999-01/2008-12) and Tongfang (2003-01/2008-12) was retrieved using key words of “adipose tissue-derived mesenchymal stem cells, adipose mesenchymal stem cells, adipose stem cell; osteogenic induction, osteogenic inducement, bone induction, osteoblastic induced; chondroblast induction, cartilage induction; bone tissue engineering, tissue engineering bone, tissue engineering of bone”. RESULTS AND CONCLUSION: A total of 361 literatures were collected, including 246 in Chinese and 115 in English. Totally 29 literatures were accordant with the study criteria. ADSCs is a truly multi-directional differentiation potential cells, which possess strong amplification and self-renewal potential, and can be directional differentiated into osteoblasts, cartilage cells, bone cells and muscle cells. It can be used as seed cells in bone tissue engineering when matching appropriate stents. Related Articles | Metrics

Select

Bone marrow-derived mesenchymal stem cells and exercise-induced myocardial cell apoptosis Fang Li-qin, Qu Hong-lin 2010, 14 (1):  171-174.  doi: 10.3969/j.issn.1673-8225.2010.01.036 Abstract ( 301 )   PDF (436KB) ( 530 )   Save BACKGROUND: Exercise-induced myocardial cell apoptosis and signal transduction and regulation mechanisms of sports medicine research have become an important issue, but remain poorly understood. Studies concerning the protective effect of bone marrow-derived mesenchymal stem cells (MSCs) to the exercise-induced myocardial cell apoptosis are rarely conducted. OBJECTIVE: To analyze the protective effect of MSCs on myocardial apoptosis and the exercise-induced myocardial cell apoptosis, so as to explore the pathological cause of exercise-induced myocardial apoptosis and the protective effects of MSCs on myocardial cell apoptosis induced by hypoxia, ischemia, and oxidative stress. METHODS: A computer-based online search of articles was performed in Medline database (1994-01/2009-09) with “Mesenchymal stem cells, Excessive exercise, Cardiomyocyte, Apoptosis” as key words and Chongqing PubMed Result NCBI database (1994-01/2009-09), Tsinghua Tong Fang database (1994-01/2009-06), with “bone marrow mesenchymal stem cells, over-training, myocardial cells, apoptosis” as key words. RESULTS AND CONCLUSION: A total of 365 articles on stem cells and exercise-induced myocardial apoptosis were collected, including 120 Chinese and 245 English. Outdate, repetitive and similar studies were excluded, and 68 were included. MSCs display a protection to myocardial cell apoptosis induced by hypoxia, ischemia and oxidative stress caused by high-intensity or overload exercise training, thereby contributing to the improvement of heart function and early rehabilitation of exercise-induced myocardial tissue diseases. Related Articles | Metrics

Select

Application of neural stem cells in nervous system diseases Meng Hong-qi, Quan Ya-ping 2010, 14 (1):  175-178.  doi: 10.3969/j.issn.1673-8225.2010.01.037 Abstract ( 381 )   PDF (468KB) ( 760 )   Save BACKGROUND: The clinical application of neural stem cells (NSCs) in combination with gene therapy could be used to treat a variety of nervous system hereditary and acquired diseases. However, nervous system diseases are varying. Nerve tissue internal environment of distinctive diseases is also different. All these factors affect the therapeutic effect of NSCs. Moreover, interaction and regulation of a variety of exogenous genes in NSC gene therapy would greatly promote continued regeneration of nerve tissue. OBJECTIVE: To review application of NSCs in the nervous system diseases. METHODS: A computer-based online search of Medline database (2000-01/2009-08) and Tongfang database (2000-01/2009-08) was performed for related articles with key words “neural stem cells, gene, nervous system diseases”. RESULTS AND CONCLUSION: A total of 88 English articles were collected. By reading the title and summary, 20 unrelated articles and 28 repetitive articles were excluded. Finally, 40 were reviewed. NSC as a new-type treatment has been used for somatic cell or transgenic vector, and has achieved a certain effect for cerebrovascular disease, brain damage disease, spinal cord injuries, neurodegenerative diseases, brain tumors, and genetic metabolic diseases. However, many key issues such as NSC proliferation, differentiation, migration and control mechanism have not been resolved. In addition, the microenvironment in nerve tissues with different diseases also influences NSC therapy. Related Articles | Metrics

Select

In vitro biological characteristics and immune regulation defects of bone marrow-derived mesenchymal stem cells from patients with aplastic anemia He Li, Xiao Yang 2010, 14 (1):  179-182.  doi: 10.3969/j.issn.1673-8225.2010.01.038 Abstract ( 288 )   PDF (441KB) ( 606 )   Save BACKGROUND: Previous research has indicated that bone marrow-derived mesenchymal stem cells had immunological regulation and hematopoiesis role. There were significant differences in immunological regulation and hemopoietic function between patients with aplastic anemia and normal cases. OBJECTIVE: To summarize the in vitro biological characteristics and its immune regulation defects of bone marrow-derived mesenchymal stem cells from patients with aplastic anemia. METHODS: A computer-based online search was conducted in PUMMED (1987-2009) and VIP database (1989-2009) with the key words of “mesenchymal stem cell, bone marrow” in both Chinese and English. There were 55 articles in total. Articles which were related to biological characteristics and its immune regulation defects of bone marrow-derived mesenchymal stem cells were included; however, duplicated articles were excluded. Finally, 32 articles were included, containing 3 reviews in English, 23 original articles in English, and 6 original articles in Chinese. RESULTS AND CONCLUSION: Bone marrow-derived mesenchymal stem cells had the capacities of high proliferation, self-renewal and multilineage differentiation; in addition they had the roles of supporting hematopoiesis, promoting implantation and hematopoietic reconstitution in vivo. Bone marrow-derived mesenchymal stem cells still had the effects of negative immune regulation and reducing the incidence of graft-versus-host disease (GVHD). Aplastic anemia correlated with hematopoietic stem cells in the pathogenesis of intrinsic defects in the proliferation or differentiation of hematopoietic microenvironment and immune system abnormalities and other related disorders. Bone marrow-derived mesenchymal stem cells of patients with aplastic anemia played an important role in the pathogenesis of this disease. Related Articles | Metrics

Select

Autologous bone marrow stem cell transplant versus autologous iliac bone graft for bone nonunion treatment Yuan Jin-guo, Zhou Zhi-ling, Liu Ying-fei, Zhu Zhen-an 2010, 14 (1):  183-186.  doi: 10.3969/j.issn.1673-8225.2010.01.039 Abstract ( 290 )   PDF (228KB) ( 559 )   Save BACKGROUND: The bone marrow stem cell (MSC) transplant treatment have the obvious superiority to tradition graft treatment for bone nonunion, but how to obtain the concentrated and highly effective bone marrow mesenchymal stem cell, as well as the dose-effect relations to fracture healing need further discussions. OBJECTIVE: To observe the curative effect of bone nonunion by using autologous MSC transplant treatment, and to compare with autologous iliac bone graft. DESIGN, TIME AND SETTING: Randomized controlled analysis was performed from January 1999 to June 2005 in the Affiliated Second Hospital of Hebei Northern College. PARTICIPANTS: The admitting 140 patients with humerus and tibia fracture were divided into 2 groups at random, autologous iliac bone graft group and autologous MSC transplant group, with 70 patients in each group. METHODS: Under aseptic condition, autologous MSC transplant group received puncture through posterior superior iliac spine, extracting bone marrow 10-20 mL from different spots, separating MSC using the density gradient centrifugation method, and counting as 4×109 nucleated cells/mL under the microscope for later use. In the autologous iliac bone graft group, bone fracture end was implanted with the suitable amount of iliac bone, while autologous MSC transplant group with the mixture of decalcified bone matrix and MSC, followed by suture. After the transplantation, external fixation may assist for 4-6 weeks according to the fixed degree of internal fixation. MAIN OUTCOME MEASURES: ① Bone callus formation and pain conditions in 2 groups at different time points after transplantation. ② Comparison of bone healing time between 2 groups. ③ Adverse events and side effects. RESULTS: According to intention-treatment analysis, experimental adopted 140 patients of humerus and tibia fractures, who all entered the final analysis. ① Bone callus formation and pain at different time points post-surgery: At 1 month after transplantation, bone callus formation in the fracture end was not obvious in autogenous iliac bone graft group, and could be seen in autologous MSC transplant group, both groups of fractures exhibited tenderness. At 2 months after transplantation, bone callus formation was observed in autogenous iliac bone graft group, fracture tenderness was relieved compared with the previous condition; in autologous MSC transplant group, a large number of bone callus formed, fracture tenderness was not obvious. At 3 months after transplantation, there were a large number of bone callus formations in autogenous iliac bone graft group, with slight fracture tenderness; in the autologous MSC transplant group, continuous bone callus formation appeared, without fracture tenderness. ② Bone healing time: The average healing time of autologous MSC transplant group was significantly shorter than autogenous iliac bone graft group [(5.5±1.5), (8.0±2.0) months, P < 0.05]. ③ Adverse events and side effects: Except 4 patients had iliac bone pain, all patients during the treatment had no infection and other complications, there were no re-fracture occurred at the follow-up of 8 months. CONCLUSION: The autologous MSC transplant treatment of exhibits a short duration and good effect for bone non-union, has obvious advantages over traditional bone graft. Related Articles | Metrics

Select

Effect and mechanism of glycosylphosphatidilinoditol- specific phospholipase D on the adhesion function of bone marrow mononuclear cells separated from myeloid leukemia patients Xiao Guang-fen, Chen Fang-ping, Fu Bin, Wang Guang-ping, Jian Zai-fu 2010, 14 (1):  187-190.  doi: 10.3969/j.issn.1673-8225.2010.01.040 Abstract ( 332 )   PDF (308KB) ( 530 )   Save BACKGROUND: There still is rarely report about the effect of glycosylphosphatidilinoditol-specific phospholipase D (GPI-PLD) on the adhesion function of leukemic cells through screening Medline and CNKI databases. OBJECTIVE: To observe the effect of GPI-PLD on the adhesion function of bone marrow mononuclear cells separated from myeloid leukemia patients, and to investigate the related mechanism. DESIGN, TIME AND SETTING: This study addressing cytology in vitro was conducted at the Hematological Laboratory of Xiangya Hospital from January to June 2004. MATERIALS: Bone marrow was collected from myeloid leukemia patients at the Department of Hematology, Xiangya Hospital, China. METHODS: The GPI-PLD activity of bone marrow mononuclear cells separated from myeloid leukemia patients was measured by using GPI-anchored placental alkaline phosphatase as substrate and Triton-X114 partition. By use of 1,10-phenanthroline, the activity of GPI-PLD was inhibited, the experiment was divided into 2 groups: treatment group adding phenanthroline to achieve a final concentration of 1 mmol/L, while control group adding the same amount of phosphate buffered saline. The adhesion rate to the fibronectin and CD24 expression of these cells were measured by MTT and immunohistochemical method, respectively. MAIN OUTCOME MEASURES: GPI-PLD activity of myeloid leukemic cells, cell adhesion rate, CD24 expression were all measured. RESULTS: The GPI-PLD activity of bone marrow mononuclear cells separated from myeloid leukemia patients was inhibited significantly after these cells were treated by 1 mmol/L 1,10-phenanthroline for 5 hours compared with control groups [(5.40±2.96)%, (42.08±7.21)%, P < 0.01]. At the same time, the adhesion rate of these cells were increased after the GPI-PLD activity was inhibited [(61.19±29.14)%, (49.78±26.73)%, P < 0.01], and the CD24 expression was also up-regulated [(18.5±11.14)%, (16.02±9.68), P < 0.01]. CONCLUSION: The adhesion rate of bone marrow mononuclear cells separated from myeloid leukemia patients can be promoted by inhibiting GPI-PLD activity. At the same time, the CD24 expression of GPI-anchored proteins on bone marrow mononuclear cells is improved. Related Articles | Metrics