Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (1): 15-18.doi: 10.3969/j.issn.1673-8225.2010.01.004

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Neuronal differentiation of adipose tissue-derived stromal cells

Liu Bin1, Wu Meng-hai1, Dong Jing1, Liu Ning1, Li Jian-min2, Zhang Jin-xia1, Li Shi-ying1, Wang Rui-min3, Chen Gui-liang4   

  1. 1First Department of Neurology,
    2Department of Neurosurgery, Affiliated Hospital of North China Coal Medical College, Tangshan   063000, Hebei Province, China;
    3Experimental Center of North China Coal Medical College, Tangshan   063000, Hebei Province, China;
    4Laboratory Animal Center of North China Coal Medical College, Tangshan   063000, Hebei Province, China
  • Online:2010-01-04 Published:2010-01-04
  • About author:Liu Bin★, Master, Professor, Chief physician, First Department of Neurology, Affiliated Hospital of North China Coal Medical College, Tangshan 063000, Hebei Province, China liubin919tsh@sina.com
  • Supported by:

    the Natural Science Foundation of Hebei Province, No. C2008000994*;
    the Overseas Training for Outstanding Scholars of Hebei Province, No. 2007*;
    the Scientific Research Foundation Program of Tangshan, No. 08130204a-1-21*

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Abstract:

BACKGROUND: Appropriate seed cell is important for transplantation in the treatment of cerebrovascular disease and other central nervous system disease.
OBJECTIVE: To investigate the capacity of human adipose tissue-derived stromal cells (ADSCs) to differentiate into neurons.
METHODS: The fatty tissue was harvested from removed abdominal unnecessary fat of healthy adult with no communicable disease or endocrine disease. Human ADSCs were isolated from human liposuction tissues and cultured in neural induction medium with GM1. Invert phase-contrast microscopy was used to observe morphology changes of ADSCs. The expression of nestin, neuron specific enolase (NSE) and microtubule-associated protein 2 (MAP2) were identified by immunocytochemistry.
RESULTS AND CONCLUSION: The majority of cells displayed typical appearance of neuronal-like cells following induction. Following 1 hours of induction, some cells began to express nestin, and NSE and MAP2-positive cells were observed at 5 hours. ADSCs can differentiate into neurons, and the differentiated neurons have the capacity of expressing nestin, NSE and MAP2.

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