Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (1): 11-14.doi: 10.3969/j.issn.1673-8225.2010.01.003

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Epidermal growth factor interferes colony formation of fibroblasts and differentiation into neuron-like cells from non-adherent bone marrow mesenchymal stem cells in mice

 Wu Yu-xin1, Wang Yan2, Ben Xiao-ming2   

  1. 1Department of Pediatric Neurosurgery, 2Neonatal Medical Center, Nanjing Children’s Hospital, Nanjing Medical University, Nanjing   210008, Jiangsu Province, China
  • Online:2010-01-04 Published:2010-01-04
  • Contact: Ben Xiao-ming, Doctor, Professor, Chief physician, Neonatal Medical Center, Nanjing Children’s Hospital, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China benxm@163.com
  • About author:Wu Yu-xin, Associate chief physician, Department of Pediatric Neurosurgery, Nanjing Children’s Hospital, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China wuyuxin21818@163.com
  • Supported by:

    the General Program of the National Natural Science Foundation of China, No. 30471836*; Key Program of Health Bureau of Jiangsu Province, No. 2004-04*

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Abstract:

BACKGROUND: Non-adherent mesenchymal stem cells (NA-MSCs) can form colony forming unit of fibroblasts and induce the differentiation into adipocytes, osteoblasts, and chondrocytes.
OBJECTIVE: To determine the effect of epithelial growth factor (EGF) on colony formation of fibroblasts and differentiation into neuron-like cells from NA-MSCs.
METHODS: Bilateral femur and tibia as well as total MSCs were separated, and repeated-transfer was employed to purify NA-MSCs. The fifth-passage total MSCs and NA-BMCs were induced in a medium containing EGF and basic fibroblast growth factor (b-FGF) for 2 weeks. Colony unit formation of fibroblasts, effect of EGF on colony-forming unit of fibroblasts, and relative protein expression detected by toluidine blue and immunocytochemical staining were observed.
RESULTS AND CONCLUSION: Both total MSCs and NA-BMC could generate colony-forming unit of fibroblasts. After treatment of EGF, colony-forming unit of fibroblasts from NA-BMC was increased significantly. Immunocytochemical staining demonstrated that two weeks later both neuro-specific NeuN and NF-200 were observed in total MSCs and NA-BMC; while, toluidine blue staining indicated that neuron-specific Nissl body was observed in some cells. EGF can effectively promote colony-forming unit of fibroblast from NA-BMC, and repeated-transfer NA-BMC can induce differentiation into neuron-like cells.

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