Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (14): 2958-2968.doi: 10.12307/2025.602

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Ban’s Culuan Zhuyun Decoction improves oocyte quality in polycystic ovary syndrome mice

Li Mingxing1, Yue Xiaolei1, Chen Xiurong2, Li Kangmei3, Liu Yunjia1, Wu Liming1, Huang Yulin1, Wu Yuanyuan4, Bai Lin1, Pan Qiaoli1, He Guozhen1, Yang Sufang5   

  1. 1Basic Medical College, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi Zhuang Autonomous Region, China; 2Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350004, Fujian Province, China; 3Affiliated Hospital of Guangdong Medical University, Zhanjiang 524013, Guangdong Province, China; 4Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China; 5Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530000, Guangxi Zhuang Autonomous Region, China 
  • Received:2024-04-08 Accepted:2024-06-11 Online:2025-05-18 Published:2024-09-28
  • Contact: He Guozhen, PhD, Professor, Master’s supervisor, Basic Medical College, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi Zhuang Autonomous Region, China Co-corresponding author: Yang Sufang, PhD, Professor, Doctoral supervisor, Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530000, Guangxi Zhuang Autonomous Region, China
  • About author:Li Mingxing, PhD, Lecturer, Basic Medical College, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi Zhuang Autonomous Region, China Yue Xiaolei, MS, Basic Medical College, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi Zhuang Autonomous Region, China Li Mingxing and Yue Xiaolei contributed equally to this work.
  • Supported by:
    the National Natural Science Foundation of China, No. 81960794 (to HGZ); PhD Introduction Research Startup Foundation of Guangxi University of Chinese Medicine, No. 2020BS032 (to YSF); Young and Middle-aged Teachers Fundamental Ability Enhancement Project in Guangxi Universities, No. 2022KY0290 (to HYL); Nanning Liangqing District Science and Technology Program Project, No. 202408 (to YSF); Undergraduate Research Training Project of Guangxi University of Chinese Medicine, No. 2023DXS04 (to PQL)

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Abstract: BACKGROUND: It is urgent to improve the study on the molecular mechanism of Ban’s Culuan Zhuyun Decoction improving oocyte quality in polycystic ovary syndrome.
OBJECTIVE: To observe the effects of Ban’s Culuan Zhuyun Decoction on oocyte quality in a mouse model of polycystic ovary syndrome and to explore the underlying mechanisms of its intervention in polycystic ovary syndrome.
METHODS: Subcutaneous injection of dehydroepiandrosterone sulfate was used to establish the polycystic ovary syndrome model in 21-day-old female Kunming mice, and the treatment was conducted for 21 consecutive days. The estrous cycle and pregnancy was recorded. ELISA was used to detect serum sex hormone levels. The rate of apoptosis in oocytes was detected using Annexin V staining. The level of reactive oxygen species in oocytes was detected using dichlorodihydrofluorescein diacetate. The condition of spindle bodies and chromosomes in oocytes were detected using the immunofluorescence method. Network pharmacology and molecular docking were used to verify the binding properties of Ban’s Culuan Zhuyun Decoction core active components and oocyte maturation-related factors (growth differentiation factor 9 and bone morphogenetic protein 15). Real-time fluorescence quantitative PCR and western blot were used to detect the mRNA and protein expression levels of growth differentiation factor 9 and bone morphogenetic protein 15 in oocytes, respectively.
RESULTS AND CONCLUSION: (1) Ban’s Culuan Zhuyun Decoction core active components (quercetin, kaempferol, and β-sitosterol) showed good binding activities with growth differentiation factor 9 and bone morphogenetic protein 15. (2) Ban’s Culuan Zhuyun Decoction ameliorated the estrous cycle, regulated serum hormone, increased the pregnancy, decreased the rate of apoptosis, declined the level of reactive oxygen species, diminished the rate of abnormal spindle assembly and chromosome loss (P < 0.01, P < 0.05); and promoted the mRNA and protein expression of growth differentiation factor 9 and bone morphogenetic protein 15 (P < 0.05). Therefore, Ban’s Culuan Zhuyun Decoction may improve the oocyte quality and increase the fertility of polycystic ovary syndrome mice by regulating the gene expression of growth differentiation factor 9 and bone morphogenetic protein 15.

Key words: Ban’s Culuan Zhuyun Decoction, polycystic ovary syndrome, mouse oocyte, in vitro maturation, growth differentiation factor 9, bone morphogenetic protein 15, pregnancy, infertility

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