Tangbikang reduces sciatic nerve oxidative stress injury in a rat model of diabetic peripheral neuropathy

doi:10.12307/2023.826

Abstract

Abstract: BACKGROUND: Tangbikang is a Traditional Chinese Medicine compound that can improve blood glucose and alleviate diabetic peripheral neuropathy. However, its regulatory mechanism is still unclear.
OBJECTIVE: To observe the effect of Tangbikang on oxidative stress of the sciatic nerve in diabetic rats and to explore the targeted regulatory mechanism of Tangbikang in alleviating diabetic peripheral neuropathy.
METHODS: Thirty Sprague-Dawley rats were randomly divided into normal group, model group and Tangbikang group, with 10 rats in each group. Animal models of diabetic peripheral neuropathy were established in the model group and Tangbikang group. After modeling, normal saline or Tangbikang (2.5 g/kg per day) was administered orally for 12 weeks. Body mass and fasting blood glucose levels of rats were detected at 0, 4, 8 and 12 weeks after administration. Hot plate test and Von Frey test were used to detect the thermal and mechanical pain thresholds of rats at 12 weeks after administration. Sciatic nerve conduction velocity and blood flow velocity were detected before sampling. Hematoxylin-eosin staining and transmission electron microscopy were used to observe the morphological changes of the sciatic nerve. The expression levels of superoxide dismutase, malondialdehyde, interleukin 1β, and tumor necrosis factor α in the sciatic nerve were detected by colorimetric method and ELISA kit. The expression levels of PI3K/AKT/FoxO1 pathway related proteins in the sciatic nerve tissue were detected by western blot assay.
RESULTS AND CONCLUSION: The fasting blood glucose level of rats in the Tangbikang group was significantly lower than that in the model group at 4, 8, and 12 weeks after modeling (P < 0.05), and the body mass of rats in the model group was significantly lower than that in the Tangbikang group (P < 0.05). Compared with the model group, the mechanical pain threshold and thermal pain threshold were significantly improved in the Tangbikang group 12 weeks after modeling (P < 0.05), and the sciatic nerve conduction velocity and blood flow velocity in the Tangbikang group were significantly higher than those in the model group
(P < 0.05). The order, density and myelination of nerve fibers in the Tangbikang group were better than those in the model group. ELISA and colorimetric results showed that compared with the model group, the levels of malondialdehyde, interleukin 1β, and tumor necrosis factor α proteins in the sciatic nerve tissue were significantly decreased in the Tangbikang group, while the level of superoxide dismutase protein was significantly increased (P < 0.05). Western blot results showed that the expressions of PI3K, p-Akt/AKT, and p-FoxO1/FoxO1 proteins in the sciatic nerve of the Tangbikang group were significantly higher than those of the model group (P < 0.05). To conclude, Tangbikang improves sciatic nerve function and reduces sciatic nerve oxidative stress injury in diabetic rats, and the mechanism may be related to the regulation of PI3K/AKT/FoxO1 pathway by Tangbikang.

Key words: Tangbikang, diabetic peripheral neuropathy, mitochondria, oxidative stress, PI3K/AKT/FoxO1 signaling pathway

CLC Number:

Jiang Shengyuan, Zhang Yaqi, Bi Jingxin, Zhang Chengfei, Zhang Qiue, Mu Xiaohong, Bai Huizhong, Zuo Xinwei, Liu Tonghua, Qin Lingling. Tangbikang reduces sciatic nerve oxidative stress injury in a rat model of diabetic peripheral neuropathy[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(32): 5162-5167.

Figures/Tables 9

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