Effect of nanonized freshwater pearl powder on the expression of osteogenic related genes

doi:10.12307/2022.863

Abstract

Abstract: BACKGROUND: The high content of calcium ions in pearls can promote calcium salt deposition, inhibit the bone resorption activity of osteoclasts, and promote bone regeneration. The water-soluble protein contained in pearls has an osteoinductive effect and can promote the differentiation of osteoblasts.
OBJECTIVE: To observe the effect of nanonized freshwater pearl powder on the expression of osteogenic related genes in osteoblasts.
METHODS: The mouse osteoblast MC3T3-E1 cells at passage 3 were taken and co-cultured with nano freshwater pearl powder (experimental group) and nano-hydroxyapatite (control group). The cells cultured separately were used as negative controls. At 7 days after culture, RT-PCR test was adopted to test the mRNA expression of Runx-related transcription factor II (Runx 2), osteopontin, and collagen type I.
RESULTS AND CONCLUSION: (1) The mRNA expression of Runx2 and osteopontin increased in the experimental group and control group compared with the negative control group (P < 0.05). The mRNA expression of Runx2 and osteopontin was higher in the experimental group than that in the control group (P < 0.05). (2) The mRNA expression of collagen type I was higher in the experimental group than that in the control group and negative control group (P < 0.05). The difference in mRNA expression of collagen type I was not significantly different between the control group and negative control group (P > 0.05). (3) These results suggest that nanonized freshwater pearl powder can significantly promote the expression of Runx2, osteopontin, and collagen type I mRNA better than nano-hydroxyapatite.

Key words: nanonized freshwater pearl powder, nanonized hydroxyapatite, osteoblast, osteogenic related genes, osteopontin, collagen type I

CLC Number:

Liao Jun, Xu Pu. Effect of nanonized freshwater pearl powder on the expression of osteogenic related genes[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(27): 4325-4329.

Figures/Tables 5

References

[1] ZHANG Z. Bone Regeneration by Stem Cell and Tissue Engineering in Oral and Maxillofacial Region. Front Med. 2011;5(4):401-413.
[2] YIN N, ZHU L, DING L, et al. MiR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells. Cell Mol Biol Lett. 2019;24(1):51.
[6] 金晔丽,潘海华,唐睿康.仿生矿化与硬组织修复[J].无机化学学报,2020,36(6):95-108.
[7] LOPEZ E, VIDAL B, BERLAND S, et al. Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro. Tissue Cell. 1992;24(5):667-679.
[8] WANG X, LIU S, XIE L, et al. Pinctada fucata mantle gene 3 (PFMG3) promotes differentiation in mouse osteoblasts (MC3T3-E1). Comp Biochem Physiol B Biochem Mol Biol. 2011;158(2):173-180.
[9] 夏静芬,钱国英,陈亮,等.珍珠粉和贝壳粉的化学成分和结构特征分析[J].化学研究与应用,2010,22(11):1467-1471.
[10] 李娜, 徐普, 陈灼庚,等.纳米珍珠粉人工骨修复兔股骨远端缺损[J].中南大学学报(医学版),2020,45(6):684-692.
[11] YANG YL, CHANG CH, HUANG CC, et al. Osteogenic Activity of Nanonized Pearl Powder/Poly (Lactide-Co-Glycolide) Composite Scaffolds for Bone Tissue Engineering. Biomed Mater Eng. 2014;24(1): 979-985.
[12] WANG X, YU T, CHEN G, et al. Preparation and Characterization of a Chitosan/Gelatin/Extracellular Matrix Scaffold and Its Application in Tissue Engineering. Tissue Eng Part C Methods. 2017;23(3):169-179.
[13] CAO M, ZHOU Y, MAO J, et al. Promoting osteogenic differentiation of BMSCs via mineralization of polylactide/gelatin composite fibers in cell culture medium. Mater Sci Eng C Mater Biol Appl. 2019;100:862-873.
[14] CHENG Y, ZHANG W, FAN H, et al. Watersoluble nanopearl powder promotes MC3T3E1 cell differentiation by enhancing autophagy via the MEK/ERK signaling pathway. Mol Med Rep. 2018;18(1):993-1000.
[15] WANG J, WANG M, CHEN F, et al. Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced Osteogenesis Via BMP/Smad Signaling Pathway. Int J Nanomedicine. 2019;14: 7987-8000.
[16] ZHANG W, WU Y, SHIOZAKI Y, et al. miRNA-133a-5p inhibits the expression of osteoblast differentiation-associated markers by targeting the 3’ UTR of RUNX2. DNA Cell Biol. 2018;37(3):199-209.
[17] LI Q, WANG Z. Involvement of FAK/P38 Signaling Pathways in Mediating the Enhanced Osteogenesis Induced by Nano-Graphene Oxide Modification on Titanium Implant Surface. Int J Nanomedicine. 2020;15:4659-4676.
[18] STACEY RB, HUNDLEY WG. Integrating Measures of Myocardial Fibrosis in the Transition from Hypertensive Heart Disease to Heart Failure. Curr Hypertens Rep. 2021;23(4):1-10.
[19] CARVALHO MS, CABRAL JM, DA SILVA CL, et al. Syner‐gistic effect of extracellularly supplemented osteo‐pontin and osteocalcin on stem cell proliferation,osteogenic differentiation, and angiogenic properties. J Cell Biochem. 2019;120(4):6555-6569.
[20] SI J, WANG C, ZHANG D, et al. Osteopontin in Bone Metabolism and Bone Diseases. Med Sci Monit. 2020;26:e919159.
[21] KAARTINEN MT, PIRHONEN A, LINNALA-KANKKUNEN A, et al. Cross-linking of Osteopontin by Tissue Transglutaminase Increases Its Collagen Binding Properties. J Biol Chem. 1999;274(3):1729.
[22] AMARJOT S, GURVEEN G, HARSIMRAT K, et al. Role of osteopontin in bone remodeling and orthodontic tooth movement: a review. Prog Orthod. 2018;19(1):18.
[23] KUSUYAMA J, BANDOW K, OHNISHI T, et al. Osteopontin inhibits osteoblast responsiveness through the downregulation of focal adhesion kinase mediated by the induction of low molecular weight-protein tyrosine phosphatase. Mol Biol Cell. 2017;28(10):1326-1336.
[24] HULTENBY K , REINHOLT FP, NORGÅRD M, et al. Distribution and synthesis of bone sialoprotein in metaphyseal bone of young rats show a distinctly different pattern from that of osteopontin. Eur J Cell Biol. 1994;63(2):230-239.
[25] 冯丽华,王敏,夏海斌,等.骨桥蛋白对巨噬细胞极化影响的体外实验研究[J].口腔医学研究,2019,35(5):497-501.
[26] ROEDER E, MATTHEWS BG, KALAJZIC I. Visual reporters for study of the osteoblast lineage. Bone. 2016;92:189-195.
[27] NIRAMITCHAINON C, MONGKORNKARN S, SRITARA C, et al. Trabecular bone score, a new bone quality index, is associated with severe periodontitis. J Periodontol. 2020;91(10):1264-1273.
[28] GRAFE I, ALEXANDER S, PETERSON JR, et al. TGF-β Family Signaling in Mesenchymal Differentiation. Cold Spring Harb Perspect Biol. 2018; 10(5): a022202.
[29] MARUELLI S, BESIO R, ROUSSEAU J, et al. Osteoblasts mineralization and collagen matrix is conserved upon specific Col1a2 silencing. Matrix Biology Plus. 2020:100028.

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