Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (17): 2726-2731.doi: 10.12307/2022.542
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Xu Xuezhen, Song Lixian, Li Aiqun, Yang Jian, Li Xiaokun, Wang Zhanqing, Shi Qingpeng
Received:
2021-06-15
Revised:
2021-06-17
Accepted:
2021-08-14
Online:
2022-06-18
Published:
2021-12-27
Contact:
Shi Qingpeng, Master, Associate chief physician, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong Province, China
About author:
Xu Xuezhen, Master, Associate chief physician, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong Province, China
Supported by:
CLC Number:
Xu Xuezhen, Song Lixian, Li Aiqun, Yang Jian, Li Xiaokun, Wang Zhanqing, Shi Qingpeng. Bone morphogenetic protein 7 inhibits apoptosis of nucleus pulposus cells[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(17): 2726-2731.
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2.4 骨形态发生蛋白7可以调控髓核细胞内衰老相关基因的转录和翻译水平 与对照髓核细胞相比,骨形态发生蛋白7处理后的人椎间盘髓核细胞中,凋亡相关基因p16和p53的mRNA表达均下调,并且骨形态发生蛋白7对p16和p53基因的抑制作用可以被抑制剂LY294002所逆转,见图4A。进一步通过免疫印迹检测衰老标志物的蛋白质表达情况,发现在骨形态发生蛋白7处理的人椎间盘髓核细胞中p16和p53的蛋白质表达均被下调,但是抑制剂LY294002可以提高被抑制的p16和p53的蛋白表达水平,见图4B。同时检测PI3K/Akt信号通路的关键信号分子p-Akt和PI3K,证实骨形态发生蛋白7通过激活PI3K/Akt信号通路参与调控椎间盘髓核细胞的抗凋亡作用,见图4C,D。"
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