Acellular nerve scaffold combined with bone marrow mesenchymal stem cells and platelet-rich gel for femoral nerve injury

doi:10.12307/2022.005

Abstract

Abstract: BACKGROUND: The use of allogeneic acellular nerve scaffold combined with bone marrow mesenchymal stem cells and platelet-rich gel to treat rabbit sciatic nerve injury is currently less reported and the mechanism is less clear.
OBJECTIVE: To evaluate the effect of transplantation of acellular nerve scaffold combined with bone marrow mesenchymal stem cells and platelet-rich gel in the rabbit sciatic nerve injury model.
METHODS: Twenty-five New Zealand rabbits were randomly divided into five groups: stump anastomosis group, acellular nerve scaffold group, acellular nerve scaffold combined with platelet-rich gel group, acellular nerve scaffold combined with bone marrow mesenchymal stem cell group, acellular nerve scaffold combined with platelet-rich plasma gel and bone marrow mesenchymal stem cell group. At 12 weeks after operation, the wet weight of gastrocnemius and histopathological staining experiments were performed.
RESULTS AND CONCLUSION: The wet weight of gastrocnemius in the acellular nerve scaffold combined with platelet-rich gel and bone marrow mesenchymal stem cell group was significantly higher than that in the other groups (P < 0.05); moreover, there were many regenerated nerve fibers, no inflammatory cell infiltration, and positive expression of neurofilament protein NF-200. It is indicated that the combination of acellular nerve scaffold and bone marrow mesenchymal stem cells and platelet-rich gel has a good effect on the regeneration of peripheral nerve injury.

Key words: stem cells, acellular nerve, bone marrow mesenchymal stem cells, platelet-rich gel, femoral nerve injury, peripheral nerve injury, rabbit

CLC Number:

Zhang Xuelei, Luo Gan, Yu Shenghui, Gu Zuchao, Peng Xu, He Xueling, Liu Yan, Zhang Xiaomei. Acellular nerve scaffold combined with bone marrow mesenchymal stem cells and platelet-rich gel for femoral nerve injury[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(1): 27-32.

Figures/Tables 5

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