Changes in bone morphology, bone mineral density and bone metabolism in different parts of natural aging rats

doi:10.12307/2021.108

Abstract

Abstract:

BACKGROUND: Rats are a kind of quadruped rodents, with the bone weight being different from that of humans who walk upright, and the time course of bone homeostasis damage within the maximum life span of rats is also inconsistent with that of humans.

OBJECTIVE: To explore the changes inbone metabolism in different rats during natural aging.
METHODS: A total of 80 Sprague-Dawley (SD) rats (male and female half) were randomly assigned into 7-,10-,15-,20-month groups according to their sex (n=10 per group). The study protocol was approved by the Animal Ethics Committee of Fuzhou Second Hospital Affiliated to Xiamen University with an approval No. 20160012 in June 2016.
RESULTS AND CONCLUSION: The bone trabeculae of female and male SD rats at 7 to 10 months of age arranged regularly and formed a uniform dense network.The bone trabeculae of femaleSD rats at 15 to 20 months of age were fine, the spacing was widened, the arrangement was scattered and sparse, and there were many discontinuities.The bone trabeculae of male SD rats at 15 months of age were slightly smaller, the spacing was widened, and the arrangement was still orderly, and the bone trabeculae of male SD rats at 20 months of age were partially interrupted.The percentage of bone trabecular area in all parts of female and male SD rats began to decrease at 10 months of age, and reached the lowest value at 20 months of age.The bone mineral density also showed the same trend.The bone alkaline phosphatase level of female and male SD rats decreased gradually with the increase of month age.The level oftype 1 collagen carboxy terminal peptide in female SD rats increased gradually with the increase of month age, while that of male SD rats began to decrease gradually at 10 months of age with the increase of month age.To conclude, 7, 10, 15, and 20 months old can represent four different stages: bone growth balance, beginning to decline, decline accelerated, and complete decline, respectively.Female SD rats at 15-20 months of agewere in a high-transition state of bone metabolism. Male SD rats at 15-20 months of agewere in a low-transition state.

Key words: Sprague-Dawleyrat, sex, osteoporosis, natural aging, bone mineral density, bone metabolism, histomorphology, bone formation, bone absorption

CLC Number:

You Bin, Huang Xiuzhu, Lin Yu, Huang Xi. Changes in bone morphology, bone mineral density and bone metabolism in different parts of natural aging rats[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(26): 4118-4122.

Figures/Tables 6

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