Influence of plasma spraying and electrochemical deposition of hydroxyapatite coating morphology on bone marrow mesenchymal stem cells

doi:10.12307/2021.067

Abstract

Abstract: BACKGROUND: With its excellent biocompatibility and osteoconductivity, hydroxyapatite is widely used as a material coating in tissue engineering, and its biological properties are strongly associated with the morphology caused by the manufacturing process.
OBJECTIVE: To investigate the effects of plasma spraying and electrochemical deposition on the stent of hydroxyapatite coating on the proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells.
METHODS: Hydroxyapatite coating was prepared on titanium stent using plasma spraying and electrochemical deposition. The titanium stent without coating was used as a control. Rat bone marrow mesenchymal stem cells were cocultured with three kinds of stents for 1, 3, 5, and 7 days. Cell proliferation was detected using CCK-8 assay for 5 days. Cell adhesion was observed using scanning electron microscope and confocal laser microscope. At 14 days, alkaline phosphatase staining was conducted; at 21 days, alizarin red staining was performed. At 14 and 21 days, real-time quantitative fluorescent polymerase chain reaction was conducted to analyze osteogenic differentiation of bone marrow mesenchymal stem cells.
RESULTS AND CONCLUSION: (1) With the extension of the culture time, the cell proliferation rate of each group increased. The cell proliferation rate of the plasma spraying group was higher than that of the electrochemical deposition group and the control group (P < 0.05). (2) Scanning electron microscopy showed that cell adhesion was seen on the stents in the three groups. The plasma spraying group had the largest cell spreading area, and the control group had the smallest cell spreading area. The laser confocal microscope showed that the actin fibers in the plasma spraying group were the densest and the cells were in good extension; the electrochemical deposition group was relatively dense and the cells were in normal extension. In the control group, cytotactin was relatively sparse and the cells were not fully extended. (3) The alkaline phosphatase activity of the plasma spraying group was higher than that of the electrochemical deposition group and the control group, and calcium nodules were formed more than that of the electrochemical deposition group and the control group. (4) Real-time quantitative fluorescent polymerase chain reaction showed that the expression levels of osteopontin, type I collagen, osteocalcin and Runx2 mRNA in plasma spraying group were higher than those in electrochemical deposition group at the same time except that there was no difference between osteocalcin mRNA expression at 7 days and Runx2 mRNA expression at 14 days (P < 0.05). (5) The results showed that the morphology of plasma sprayed hydroxyapatite coating is more conducive to proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells.

Key words: bone, material, titanium stent, hydroxyapatite, coating, plasma spraying, electrochemical deposition, bone marrow mesenchymal stem cells

CLC Number:

Sun Yang, Luo Mingran, Zheng Li, Hu Weifan, Yuan Feng. Influence of plasma spraying and electrochemical deposition of hydroxyapatite coating morphology on bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(28): 4516-4522.

Figures/Tables 8

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