Preparation of alginate-gelatin-adipose-derived stem cells microspheres by electrospray and feasibility on repairing articular cartilage injury

doi:10.12307/2021.060

Abstract

Abstract: BACKGROUND: Microsphere can be used as a cell delivery system to localize cells in cartilage injury site and regenerate them by injection or combined with other scaffolds.
OBJECTIVE: To produce adipose-derived stem cells (ADSCs)-embedded alginate-gelatin microspheres for cartilage tissue engineering by electrospray, and to evaluate cell viability, proliferation and chondrogenic differentiation ability of microspheres.
METHODS: The electrospray technology was used to produce alginate-gelatin microspheres with different gelatin concentrations (0, 5, 15, 25 g/L). The morphology of microspheres could be observed under the optical microscope. The mass concentration of gelatin to form complete microspheres was selected for the following experiments. Alginate-gelatin-ADSCs microspheres and alginate-ADSCs microspheres were produced by electrospray. The spherical effect was observed under the optical microscope. Two kinds of microspheres containing cells were cultured in rotating cell culture system. The proliferation ability, viability and chondrogenic ability of the cells in the microspheres were detected at the set time point.
RESULTS AND CONCLUSION: (1) Intact microspheres could be produced by adding 0, 5 g/L gelatin. The diameter of the microspheres was (365.85±16.88) or (358.85±23.97) μm. There was no significant difference in the diameter of the two kinds of microspheres (P > 0.05). The mass concentration of gelatin as 5 g/L was selected for subsequent experiments. (2) The addition of ADSCs did not affect the spheroidization and the diameter of alginate microspheres and alginate microspheres-gelatin microspheres. (3) The number of cell proliferation and the proportion of living cells at 7 and 14 days were higher in the alginate-gelatin-ADSCs microspheres than those without alginate-ADSCs microspheres (P < 0.05). (4) The content of glycosaminoglycan was higher in alginate-gelatin-ADSCs microspheres than that in alginate-ADSCs microspheres at 7, 14, and 21 days after chondrogenic differentiation (P < 0.05). (5) It is concluded that alginate-gelatin-ADSCs microspheres are prone to promote proliferation and keep cell viability, and are a kind of biomaterials with great potential in the field of cartilage tissue engineering.

Key words: bone, materials, alginate, gelatin, cartilage tissue engineering, biomaterials, electrospray, microcarrier

CLC Number:

Liao Sida, Meng Haoye, Li Junkang, Xu Yichi, Li Huo, Tian Xiaoyu, Feng Yong, Wang Aiyuan, Peng Jiang. Preparation of alginate-gelatin-adipose-derived stem cells microspheres by electrospray and feasibility on repairing articular cartilage injury[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(28): 4473-4479.

Figures/Tables 10

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