中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (41): 7709-7712.doi: 10.3969/j.issn.2095-4344.2012.41.022

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

骨髓间充质干细胞对衰老心脏血管内皮生长因子表达的影响

李艳菊1，刘 洋2，郭坤元3   

  1. 1贵阳医学院附属医院血液科，贵州省贵阳市 550004
    2军事医学科学院，北京市 100850
    3珠江医院血液科，广东省广州市 510282
  • 收稿日期:2012-01-03 修回日期:2012-02-18 出版日期:2012-10-07 发布日期:2013-04-09
  • 通讯作者: 郭坤元，博士，博士生导师，南方医科大学珠江医院血液科，广东省广州市 510282 wodeyoujian@foxmail.com
  • 作者简介:李艳菊☆，女，1981年生，辽宁省盘锦市人，汉族，2009年南方医科大学毕业，博士，主要从事干细胞组织工程方面的研究。 lyj8181@163.com

Effect of bone marrow mesenchymal stem cells on vascular endothelial growth factor expression in the aging heart of rats

Li Yan-ju1, Liu Yang2, Guo Kun-yuan3   

  1. 1Department of Hematology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
    2Academy of Military Medical Sciences, Beijing 100850, China
    3Department of Hematology, Zhujiang Hospital, Guangzhou 510282, Guangdong Province, China
  • Received:2012-01-03 Revised:2012-02-18 Online:2012-10-07 Published:2013-04-09
  • Contact: Guo Kun-yuan, M.D., Doctoral supervisor, Department of Hematology, Zhujiang Hospital, Guangzhou 510282, Guangdong Province, China wodeyoujian@foxmail.com
  • About author:Li Yan-ju☆, M.D., Department of Hematology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China lyj8181@163.com

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被引次数

1

摘要：

背景：骨髓间充质干细胞分泌的血管内皮生长因子可能对心脏衰老有治疗作用，但这一作用是否与其能否归巢到心脏有关呢？
目的：分析骨髓间充质干细胞对衰老心脏的影响。
方法：取大鼠骨髓间充质干细胞。40只大鼠随机数字表法均分为正常组、造模组、治疗组和荧光标记剂组。后3组皮下注射D-半乳糖建立衰老心脏模型，后2组造模后尾静脉注射未标记和经羧基荧光素乙酰乙酸标记后的骨髓间充质干细胞；观察细胞是否定位于心脏、心脏组织各因子的表达。
结果与结论：骨髓间充质干细胞能够归巢到衰老心脏；与模型组比较，骨髓间充质干细胞治疗后能够使血清与心脏中超氧化物歧化酶活性均明显增高、丙二醛含量显著降低(P < 0.05)、心脏组织血管内皮生长因子表达升高(P < 0.05)；说明骨髓间充质干细胞分泌血管内皮生长因子可能是其改善衰老心脏的机制之一。

关键词: 骨髓间充质干细胞, 归巢, 衰老, 心脏, 血管内皮生长因子

Abstract:

BACKGROUND: Vascular endothelial growth factors secreted by bone marrow mesenchymal stem cells may exhibit therapeutic effects on the aging heart. However, whether the therapeutic effects are associated with bone marrow mesenchymal stem cells homing to the heart?
OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cells on aging heart.
METHODS: Rat bone marrow mesenchymal stem cells were harvested. Forty rats were randomly divided into four groups: normal, model, treatment and fluorescence labeling. Rats in the latter three groups were subcutaneously injected with D-galactose to establish aging heart rat models. Rats in the latter two groups were injected with non-labeled or carboxyflu-orescein diacetate succinimidyl ester-labeled bone marrow mesenchymal stem cells. Cell location, cardiac tissue factor expression were investigated.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells could arrive at the aging heart. Compared with the model group, the activity of superoxide dismutase in the serum and heart tissue was significantly increased, the level of malondialdehyde was significantly decreased (P < 0.05), and vascular endothelial growth factor expression in the cardiac tissue was significantly increased after treatment with bone marrow mesenchymal stem cells. These findings suggest that bone marrow mesenchymal stem cells can secrete vascular endothelial growth factors, which may be one of mechanisms by which bone marrow mesenchymal stem cells contribute to improvement of aging heart.

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