中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6218-6222.doi: 10.3969/j.issn.1673-8225.2010.33.034

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

组织工程技术在下尿路修复重建中的应用

刘  杰，傅  强   

  1. 上海交通大学附属第六人民医院泌尿外科，上海市   200233
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 作者简介:刘 杰★，男，1984年生，汉族，山东省滨州惠民县人，苏州大学在读硕士，主要从事组织工程方面实验研究。 shalj841004@163.com

Application of tissue engineering technology in the low urinary system reconstruction

Liu Jie, Fu Qiang   

  1. Department of Urinary Surgery, Six People’s Hospital of Shanghai Jiao Tong University, Shanghai  200233, China
  • Online:2010-08-13 Published:2010-08-13
  • About author:Liu Jie★, Studying for master’s degree, Department of Urinary Surgery, Six People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China shalj841004@163.com

PDF

622

被引次数

12

摘要：

背景：下尿路损伤、炎症、肿瘤以及先天性畸形等原因常可造成下尿路组织的严重缺损。传统手术对于下尿路修复重建存在诸多弊端。组织工程利用脱细胞基质与复合种子细胞的生物支架构建组织器官，为下尿路的临床修复与重建开辟了新的治疗思路。
目的：就组织工程技术在男性下尿路修复重建中成熟技术做简单概述,并指出现阶段组织工程在临床应用之不足。
方法：以“tissue engineering，urethra engineering，bladder；urethra，testicle”为英文检索词；以“组织工程；尿道重建；膀胱；尿道；睾丸”为中文检索词。应用计算机检索1994-01/2009-06 PubMed数据库和CNKI数据库相关文章，检索到文献72篇，排除重复性研究，最终纳入29篇文献进行分析。
结果与结论：目前用膀胱替代材料重建膀胱的实验主要有两种模型，即脱细胞基质支架模型和复合种子细胞的生物支架模型。脱细胞基质支架材料主要有膀胱细胞外基质、尿道细胞外基质、小肠黏膜下基质等。复合种子细胞的生物支架则是将种子细胞接种与相应的支架上，经过相应的处理再回植入体内。这两种模型目前正处于实验与临床研究中。组织工程化尿道方面的应用技术与组织工程化膀胱类似。睾丸组织工程研究目前还处于萌芽阶段。目前组织工程“替代品”还无法与正常的组织器官相媲美，还有一定的缺陷

关键词: 组织工程, 尿道重建, 膀胱, 尿道, 睾丸

Abstract:

BACKGROUND: Injury, inflammation, tumor, congenital malformation and other reasons often cause serious defects in the lower urinary tract. There are many disadvantages of lower urinary tract reconstruction using conventional surgery. Tissue engineering technology can construct tissue engineered organs with acellular matrix and biomaterial scaffold, which exploits a new therapeutic approach for clinical reconstruction of lower urinary tract.
OBJECTIVE: To review the application of tissue engineering technology in male low urinary system reconstruction and to point out its shortcomings.
METHODS: PubMed and CNKI databases were searched using key words of “tissue engineering, urethra engineering, bladder, urethra, testicle” from January 1994 to June 2009. Totally 72 literatures were initial searched, and 29 documents were included in the further analysis.
RESULTS AND CONCLUSION: Two kinds of models were used in vesical construction, that is, acellular matrix model and composite scaffolds with seeded cells model. The acellular matrix scaffold comprised vesical extracellular matrix, urethra extracellular matrix and small intestinal submucosa, etc. In composite scaffolds with seeded cells, seed cells were incubated into scaffolds, and implanted into bodies after certain treatment. Application of urethra tissue engineering was similar to that of bladder. But testis tissue engineering is just at the sprout stage. Presently, the tissue engineered “substitute” is not comparable to natural tissues.

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