中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (30): 6474-6481.doi: 10.12307/2025.781

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

茶多酚干预运动疲劳模型小鼠的中枢抗炎作用与机制

张松江，李龙洋，周春光，高剑峰   

  1. 河南中医药大学医学院，河南省郑州市  450046
  • 收稿日期:2024-09-09 接受日期:2024-11-02 出版日期:2025-10-28 发布日期:2025-03-28
  • 通讯作者: 高剑峰，博士，教授，硕士生导师，河南中医药大学医学院，河南省郑州市 450046
  • 作者简介:张松江，女，1966年生，河南省唐河县人，汉族，2009年中南大学毕业，博士，教授，硕士生导师，主要从事运动疲劳和阿尔茨海默病病机和治疗的研究。
  • 基金资助:
    河南省重点科技攻关项目(152102310100)，项目负责人：张松江；河南省自然科学基金(202300410267)，项目负责人：高剑峰；河南中医药大学创新团队课题(校政[2016]No.124)，项目负责人：高剑峰

Central anti-inflammatory effect and mechanism of tea polyphenols in exercise fatigue model mice

Zhang Songjiang, Li Longyang, Zhou Chunguang, Gao Jianfeng   

  1. Medical College of Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China
  • Received:2024-09-09 Accepted:2024-11-02 Online:2025-10-28 Published:2025-03-28
  • Contact: Gao Jianfeng, MD, Professor, Master’s supervisor, Medical College of Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China
  • About author:Zhang Songjiang, MD, Professor, Master’s supervisor, Medical College of Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China
  • Supported by:
    Key Science and Technology Research Project in Henan Province, No. 152102310100 (to ZSJ); Henan Natural Science Foundation, No. 202300410267 (to GJF); Innovation Team Project of Henan University of Chinese Medicine, No. [2016]124 (to GJF)

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摘要：


文题释义：
茶多酚：是茶叶的主要成分，占绿茶干质量的15%-30%。茶多酚是多酚类物质的总称，包括黄烷酮类、花色素类、黄酮醇类、花白素类、酚酸和缩酚酸类等6类化合物，其中以黄烷酮类(主要是儿茶素类化合物)最为重要，占茶多酚总量的60%-80%。
小胶质细胞：是存在于中枢神经系统的一种胶质细胞，约占大脑中神经胶质细胞的20%，是中枢神经系统中的第一道也是最主要的一道免疫防线。小胶质细胞不停地清除着中枢神经系统中损坏的神经、斑块及感染性物质。但是过度激活的小胶质细胞是促炎因子和有神经毒性物质(如肿瘤坏死因子、一氧化氮和白细胞介素等)的重要来源。

背景：研究显示，茶多酚有多器官的抗炎作用，但关于茶多酚对运动疲劳所致的神经中枢炎症影响的研究较少。
目的：探讨茶多酚在运动疲劳中的中枢抗炎作用和机制。
方法：将7周龄雄性昆明小鼠随机分为安静对照组、疲劳模型组和茶多酚组。让疲劳模型组小鼠一次性力竭游泳，茶多酚组小鼠在一次性力竭游泳前30 min腹腔注射1次茶多酚。记录疲劳模型组和茶多酚组小鼠的力竭游泳时间。各组小鼠在力竭游泳2 h后取脑组织，苏木精-伊红染色法观察小鼠脑组织形态结构变化；免疫印迹或实时荧光定量聚合酶链式反应方法检测脑组织中炎症相关因子的表达、小胶质细胞活化情况及STAT3/核因子κB p65炎症通路的激活情况；酶联免疫吸附法检测血浆炎症因子水平。
结果与结论：①与疲劳模型组相比，茶多酚组小鼠游泳力竭时间明显延长；②各组小鼠的脑组织苏木精-伊红染色结果未发现异常；③与安静对照组相比，疲劳模型组小鼠肿瘤坏死因子α蛋白、白细胞介素1β蛋白、M1型活化小胶质细胞标志物诱导型一氧化氮合酶蛋白、核因子κB p65蛋白和mRNA及p-STAT3蛋白和STAT3 mRNA表达明显增多，抗炎因子白细胞介素10蛋白、M2型小角质细胞标志物精氨酸酶1蛋白表达明显减少；与疲劳模型组相比，茶多酚组小鼠炎症反应、小胶质细胞类型及信号分子等相关指标呈相反的明显变化；④各组小鼠外周血浆和脑组织炎症相关因子肿瘤坏死因子α、白细胞介素1β、白细胞介素10的表达一致；⑤结果表明，运动疲劳可以引发神经中枢炎症反应，茶多酚可以减轻这种炎症反应，继而增加小鼠的耐疲劳时间；运动疲劳和茶多酚对脑内的炎症反应的影响可能是通过STAT3/核因子κB p65通路完成的。    
https://orcid.org/0000-0002-9752-9829(张松江)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 茶多酚, 运动疲劳, 中枢炎症, 小胶质细胞, 炎症通路, 工程化组织构建

Abstract: BACKGROUND: Studies have shown that tea polyphenols have anti-inflammatory effects on multiple organs, but there are few studies on the effects of tea polyphenols on central nervous system inflammation caused by exercise fatigue. 
OBJECTIVE: To explore the central anti-inflammatory effect and mechanism of tea polyphenols in exercise fatigue.
METHODS: 7-week-old male Kunming mice were divided into quiet control group, fatigue model group, and tea polyphenols group. In the fatigue model group, mice were given exhaustive swimming at one time. In the tea polyphenols group, tea polyphenols were injected into abdominal cavity half an hour before exhaustive swimming. The exhaustive swimming time in mice of fatigue model group and tea polyphenols group was recorded. Samples were taken from mice in each group after two hours of exhaustive swimming. The changes of brain tissue morphology and structure in each group were observed by hematoxylin-eosin staining. Western blot assay or real-time fluorescence quantitative polymerase chain reaction were used to detect the expression of inflammation-related factors, the activation of microglia, and the activation of STAT3/nuclear factor-κB p65 inflammatory pathway in the brain tissue of mice. Enzyme-linked immunosorbent assay was used to detect the levels of plasma inflammatory factors.
RESULTS AND CONCLUSION: (1) Compared with the fatigue model group, the swimming exhaustion time of mice in the tea polyphenols group was significantly prolonged. (2) No abnormality was found in the hematoxylin-eosin staining results of brain tissues of mice in each group. (3) Compared with the quiet control group, the expression levels of inflammatory factors tumor necrosis factor α protein, interleukin-1β protein, M1 activated microglia marker-inducible nitric oxide synthase protein, nuclear factor-κB p65 protein and mRNA, and p-STAT3 protein and STAT3 mRNA in the fatigue model group were significantly increased, while the expression levels of anti-inflammatory factor interleukin-10 protein and M2 activated microglia marker-arginase 1 protein were significantly decreased. Compared with the fatigue model group, the inflammatory reaction, microglia types and signal molecules showed opposite obvious changes in the tea polyphenols group. (4) The expression levels of tumor necrosis factor α, interleukin 1β, interleukin-10 in peripheral plasma and brain tissue were consistent in mice of each group. (5) To sum up, exercise fatigue can trigger inflammatory reaction of nerve center, and tea polyphenols can alleviate this inflammatory reaction, and then enhance the fatigue resistance time of mice. The effects of exercise-induced fatigue and tea polyphenols on the inflammatory reaction in the brain may be completed through STAT3/nuclear factor-κB p65 pathway. 

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words:  tea polyphenols, exercise-induced fatigue, central inflammation, microglial cell, inflammatory pathway, engineered tissue construction

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