中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (2): 269-278.doi: 10.12307/2025.274

• 血管组织构建 vascular tissue construction • 上一篇    下一篇

不同强度运动干预2型糖尿病大鼠骨骼肌羧酸酯酶1及炎症因子的变化

胡淑娟1，2，程  平3，张  啸3，丁一庭3，刘  璇1，蒲  锐1，汪献旺3   

  1. 长江大学，1教育与体育学院，3医学部，湖北省荆州市  423000；2吉首大学体育科学学院，湖南省吉首市  416000
  • 收稿日期:2023-12-27 接受日期:2024-03-06 出版日期:2025-01-18 发布日期:2024-05-24
  • 通讯作者: 汪献旺，博士，教授，长江大学医学部，湖北荆州市 423000
  • 作者简介:胡淑娟，女，1987年生，湖南省郴州市人，汉族，博士，副教授，主要从事运动与健康促进方面的研究。 共同第一作者：程平，女，1992年生，安徽省铜陵市人，汉族，在读硕士，主要从事医学检验方面的研究。
  • 基金资助:
    国家自然科学基金项目(81974115)；湖北省重点科技研发计划项目(2021BGD010)，项目负责人：汪献旺；湖北省教育厅科研计划项目(D20201306)，项目负责人：汪献旺；长江大学大学生创新创业训练计划项目(Yz2022307，Yz2022164，Yz2023165)；长江大学社会科学基金重点项目(2022csz07)，项目负责人：胡淑娟

Effects of different exercise interventions on carboxylesterase 1 and inflammatory factors in skeletal muscle of type 2 diabetic rats 

Hu Shujuan1, 2, Cheng Ping3, Zhang Xiao3, Ding Yiting3, Liu Xuan1, Pu Rui1, Wang Xianwang3   

  1. 1School of Education and Physical Education, Yangtze University, Jingzhou 423000, Hubei Province, China; 2College of Sports Science, Jishou University, Jishou 416000, Hunan Province, China; 3Department of Medicine, Yangtze University, Jingzhou 423000, Hubei Province, China 
  • Received:2023-12-27 Accepted:2024-03-06 Online:2025-01-18 Published:2024-05-24
  • Contact: Wang Xianwang, MD, Professor, Department of Medicine, Yangtze University, Jingzhou 423000, Hubei Province, China
  • About author:Hu Shujuan, MD, Associate professor, School of Education and Physical Education, Yangtze University, Jingzhou 423000, Hubei Province, China; College of Sports Science, Jishou University, Jishou 416000, Hunan Province, China Cheng Ping, Master candidate, Department of Medicine, Yangtze University, Jingzhou 423000, Hubei Province, China Hu Shujuan and Cheng Ping contributed equally to this work.
  • Supported by:
    National Natural Science Foundation of China, No. 81974115; Hubei Key Research and Development Program, No. 2021BGD010 (to WXW); Hubei Science and Technology Research Program, No. D20201306 (to WXW); Yangtze University College Students Innovation and Entrepreneurship Training Programs, Nos. Yz2022307, Yz2022164, and Yz2023165; Key Project of Social Science Foundation of Yangtze University, No. 2022csz07 (to HSJ)

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摘要：

文题释义：

羧酸酯酶1：位于细胞内质网内，是调节葡萄糖稳态和脂质代谢的关键靶点，在药物代谢和解毒过程中也发挥着重要的作用，并且与炎症关系密切。
炎症因子：导致局部组织的变质、渗出和增生，是引起组织和细胞损伤的原因。2型糖尿病是以β细胞功能障碍和胰岛素抵抗为主要特征的炎症性和代谢性疾病，2型糖尿病患者的血糖、血脂等指标的上升会引起炎症因子的增加。

背景：羧酸酯酶1及炎症因子在调节脂质代谢以及葡萄糖稳态方面起着至关重要的作用，然而有关不同强度运动干预对2型糖尿病大鼠骨骼肌羧酸酯酶1及炎症因子的影响尚待揭示。
目的：探究不同强度运动干预对2型糖尿病大鼠骨骼肌羧酸酯酶1及炎症因子的影响。
方法：取32只8周龄雄性SD大鼠，适应性喂养1周后随机分为正常对照组(n=12)和造模组(n=20)，造模组大鼠利用高脂膳食和一次性注射链脲佐菌素制备2型糖尿病模型，建模成功后随机分为糖尿病对照组(n=6)、中等强度运动组(n=6)和高强度间歇运动组(n=6)，后2组适应性跑台运动5 d后分别进行对应强度的跑台运动，每天1次，每次50 min，每周训练5 d。连续运动6周后，检测大鼠血糖与血脂相关指标，苏木精-伊红染色观察骨骼肌组织形态学变化，qRT-PCR检测骨骼肌羧酸酯酶1与炎症因子的mRNA表达，Western-blotting及免疫荧光染色检测骨骼肌中羧酸酯酶1与炎症因子的蛋白表达。
结果与结论：①与正常对照组相比，糖尿病对照组大鼠空腹血糖、三酰甘油、低密度脂蛋白胆固醇、胰岛素抵抗指数均升高(P < 0.05)，胰岛素活性降低(P < 0.05)，骨骼肌中的羧酸酯酶1、NEK7、白细胞介素18的mRNA与蛋白表达均升高(P < 0.05)。②与糖尿病对照组相比，中等强度运动组和高强度间歇运动组大鼠空腹血糖、三酰甘油、低密度脂蛋白胆固醇、胰岛素抵抗指数均降低(P < 0.05)，胰岛素活性升高(P < 0.05)；中等强度运动组大鼠骨骼肌中的NEK7 mRNA表达降低(P < 0.01)，羧酸酯酶1、NEK7、白细胞介素18蛋白表达降低(P < 0.05)；高强度间歇运动组大鼠骨骼肌中的羧酸酯酶1、NEK7、NLRP3、白细胞介素18 mRNA表达降低(P < 0.05)，羧酸酯酶1、白细胞介素18蛋白表达降低(P < 0.05)。③苏木精-伊红染色显示，相较于糖尿病对照组，中等强度运动组大鼠肌纤维间隙变小，内部空洞减少，细胞结构趋于完整；高强度间歇运动组大鼠肌细胞排列松散，组织形态不规则，肌纤维内部空洞较多。④结果表明，中等强度运动和高强度间歇运动均可降低2型糖尿病大鼠的血糖、血脂、胰岛素抵抗与骨骼肌羧酸酯酶1水平，中等强度运动可明显降低骨骼肌NEK7表达，高强度间歇运动可降低骨骼肌白细胞介素18表达，并且羧酸酯酶1与NEK7、白细胞介素18关系密切。
https://orcid.org/0000-0001-6860-9080(胡淑娟)
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 中等强度有氧运动, 高强度间歇运动, 2型糖尿病, 羧酸酯酶1, 炎症因子

Abstract: BACKGROUND: Carboxylesterase 1 and inflammatory factors play a crucial role in regulating lipid metabolism and glucose homeostasis. However, the effects of different exercise intensity interventions on carboxylesterase 1 and inflammatory factors in skeletal muscle of type 2 diabetic rats remain to be revealed.
OBJECTIVE: To investigate the effects of different exercise intensity interventions on carboxylesterase 1 and inflammatory factors in skeletal muscle of type 2 diabetic rats.
METHODS: Thirty-two 8-week-old male Sprague-Dawley rats were randomly divided into normal control group (n=12) and modeling group (n=20) after 1 week of adaptive feeding. Rat models of type 2 diabetes mellitus were prepared by high-fat diet and single injection of streptozotocin. After successful modeling, the rats were randomly divided into diabetic control group (n=6), moderate-intensity exercise group (n=6) and high-intensity intermittent exercise group (n=6). The latter two groups were subjected to treadmill training at corresponding intensities, once a day, 50 minutes each, and 5 days per week. Exercise intervention in each group was carried out for 6 weeks. After the intervention, ELISA was used to detect blood glucose and blood lipids of rats. The morphological changes of skeletal muscle were observed by hematoxylin-eosin staining. The mRNA expression levels of carboxylesterase 1 and inflammatory cytokines were detected by real-time quantitative PCR. The protein expression levels of carboxylesterase 1 and inflammatory cytokines were detected by western blot and immunofluorescence.
RESULTS AND CONCLUSION: Compared with the normal control group, fasting blood glucose, triglyceride, low-density lipoprotein cholesterol, insulin resistance index in the diabetic control group were significantly increased (P < 0.01), insulin activity was decreased (P < 0.05), and the mRNA and protein levels of carboxylesterase 1, never in mitosis gene A related kinase 7 (NEK7) and interleukin 18 in skeletal muscle tissue were upregulated (P < 0.05). Compared with the diabetic control group, fasting blood glucose, triglyceride, low-density lipoprotein cholesterol, and insulin resistance index in the moderate-intensity exercise group and high-intensity intermittent exercise group were down-regulated (P < 0.05), and insulin activity was increased (P < 0.05). Moreover, compared with the diabetic control group, the mRNA level of NEK7 and the protein levels of carboxylesterase 1, NEK7 and interleukin 18 in skeletal muscle were decreased in the moderate-intensity exercise group (P < 0.05), while the mRNA levels of carboxylesterase 1, NEK7, NOD-like receptor heat protein domain associated protein 3 and interleukin 18 and the protein levels of carboxylesterase 1 and interleukin 18 in skeletal muscle were downregulated in the high-intensity intermittent exercise group (P < 0.05). Hematoxylin-eosin staining showed that compared with the diabetic control group, the cavities of myofibers in the moderate-intensity exercise group became smaller, the number of internal cavities was reduced, and the cellular structure tended to be more intact; the myocytes of rats in the high-intensity intermittent exercise group were loosely arranged, with irregular tissue shape and increased cavities in myofibers. To conclude, both moderate-intensity exercise and high-intensity intermittent exercise can reduce blood glucose, lipid, insulin resistance and carboxylesterase 1 levels in type 2 diabetic rats. Moderate-intensity exercise can significantly reduce the expression level of NEK7 protein in skeletal muscle, while high-intensity intermittent exercise can significantly reduce the expression level of interleukin 18 protein in skeletal muscle. In addition, the level of carboxylesterase 1 is closely related to the levels of NEK7 and interleukin 18.

Key words:  moderate-intensity aerobic exercise, high-intensity intermittent exercise, type 2 diabetes, carboxylesterase 1, inflammatory factor

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