中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (24): 3876-3881.doi: 10.3969/j.issn.2095-4344.2015.24.020

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

树突状细胞共刺激分子表达与免疫抑制剂的干预

杨晓勇1,姚庆春2,王  玮1,齐  曼1,洪希莹1,刘晨妹1,尹  航1,刘  航1,任  亮1,胡小鹏1,张小东1   

  1. 1首都医科大学附属北京朝阳医院泌尿外科,北京市 100020;
    2山东大学附属省立医院重症医学科,山东省济南市 250021
  • 出版日期:2015-06-11 发布日期:2015-06-11
  • 通讯作者: 张小东,博士,教授,博士生导师,首都医科大学附属北京朝阳医院泌尿外科,北京 市 100020
  • 作者简介:杨晓勇,男,1971年生,河南省信阳市人,主要从事器官移植和免疫耐受临床和基础研究。
  • 基金资助:

    国家自然科学基金专项基金(81050014)

Dendritic cell costimulatory molecule expression and immunosuppressant intervention

Yang Xiao-yong1, Yao Qing-chun2, Wang Wei1, Qi Man1, Hong Xi-ying1, Liu Chen-mei1, Yin Hang1, Liu Hang1, Ren Liang1, Hu Xiao-peng1, Zhang Xiao-dong1   

  1. 1Department of Urology, Beijing Chaoyang Hospital of Capital Medical University, Beijing 100020, China; 
    2Department of Critical Care Medicine, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China
  • Online:2015-06-11 Published:2015-06-11
  • Contact: Zhang Xiao-dong, M.D., Professor, Doctoral supervisor, Department of Urology, Beijing Chaoyang Hospital of Capital Medical University, Beijing 100020, China
  • About author:Yang Xiao-yong, Department of Urology, Beijing Chaoyang Hospital of Capital Medical University, Beijing 100020, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81050014

摘要:

背景:以往关于器官移植免疫抑制和抗排斥反应的研究,多关注T淋巴细胞介导的免疫反应及免
疫抑制剂对T淋巴细胞的作用,树突状细胞作用尚不清楚,且对于免疫抑制剂对树突状细胞影响
的表现及机制亦不尽相同。
目的:比较不同免疫抑制剂对树突状细胞共刺激分子表达及功能的影响,探讨其免疫抑制作用机
制。
方法:在诱导C57BL/6小鼠骨髓细胞定向分化为树突状细胞时分别加入20 μg/L雷帕霉素、0.04
mg/L霉酚酸酯、10 μg/L他克莫司和1 mg/L环孢霉素A。
结果与结论:流式细胞仪检测结果显示,各组CD40表达为:雷帕霉素<他克莫司<环孢霉素A<霉酚
酸酯(P < 0.01);CD86表达分别为:雷帕霉素<他克莫司<环孢霉素A =霉酚酸酯(P < 0.01);
CD80、CD11c及主要组织相容性复合体Ⅱ的表达各组差异无显著性意义(P > 0.05)。单向混合淋
巴细胞反应结果显示,各组树突状细胞共刺激T细胞增殖能力表现为:雷帕霉素<霉酚酸酯<他克
莫司=环孢霉素A (P < 0.05)。结果证实,雷帕霉素、他克莫司、环孢霉素A、霉酚酸酯均通过抑
制树突状细胞共刺激分子CD40及CD86表达而发挥免疫抑制作用,以雷帕霉素最为显著;虽然以上
药物对树突状细胞抗原递呈能力主要组织相容性复合体Ⅱ的表达均无明显抑制性,但均能抑制树

关键词:

国家自然科学基金

Abstract:

BACKGROUND: Previous studies on immunosuppression and anti-rejection after organ
transplantation mainly focused on effects of T lymphocytes-mediated immune response
and immunosuppressive agents on T lymphocytes. Effects of dendritic cells were
unclear. The manifestation and mechanism of immunosuppressive agent effects on
dendritic cells are not identical.
OBJECTIVE: To compare the effects of different immunosuppressive agents on
expression and function of costimulatory molecules of dendritic cells, and to
explore the mechanism of action of immunosuppressive agents.
METHODS: 20 μg/L rapamycin, 0.04 mg/L mycophenolate, 10 μg/L tacrolimus and 1 mg/L
cyclosporine A were separately added during bone marrow cells of C57BL/6 mice were
differentiated into dendritic cells.
RESULTS AND CONCLUSION: Flow cytometry results revealed that CD40 expression in each
group: rapamycin < tacrolimus < cyclosporine A < mycophenolate mofetil (P < 0.01);
CD86 expression: rapamycin < tacrolimus < cyclosporine A = mycophenolate mofetil (P
< 0.01). There was no significant difference in CD80, CD11c and major
histocompatibility complex II expression among groups (P > 0.05). One-way mixed
lymphocyte reaction  results displayed dendritic cell costimulatory T cell proliferation in each group:
rapamycin < mycophenolate mofetil < tacrolimus = cyclosporine A (P < 0.05). The
results confirmed that rapamycin, tacrolimus, cyclosporine A, and mycophenolate
mofetil exerted immunosuppression by suppressing CD40 and CD86, especially
rapamycin. Above medicines do not noticeably inhibit major histocompatibility
complex II expression, but suppress T cell proliferation, and the inhibitory effect

Key words: Tissue Engineering, Dendrites, Rapamycin, Lymphocytes

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