中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (22): 4694-4701.doi: 10.12307/2025.454

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

纳米金颗粒@介孔二氧化硅修饰钛种植体促进高糖条件下的成骨分化

邓云艺1，2，3，陈世超1，2，3，罗明东2，3，李若彤2，3，兰小蓉2，3，余  科1，2，3，李广文1，2，3   

  1. 1西南医科大学附属口腔医院种植科，四川省泸州市   646000；2口颌面修复重建和再生泸州市重点实验室，四川省泸州市   646000；3西南医科大学口腔医学研究所，四川省泸州市   646000
  • 收稿日期:2024-03-20 接受日期:2024-05-25 出版日期:2025-08-08 发布日期:2024-12-05
  • 通讯作者: 李广文，博士在读，主治医师，西南医科大学附属口腔医院种植科，四川省泸州市 646000；口颌面修复重建和再生泸州市重点实验室，四川省泸州市 646000；西南医科大学口腔医学研究所，四川省泸州市 646000 余科，博士，副教授，西南医科大学附属口腔医院种植科，四川省泸州市 646000；口颌面修复重建和再生泸州市重点实验室，四川省泸州市 646000；西南医科大学口腔医学研究所，四川省泸州市 646000
  • 作者简介:邓云艺，男，1998年生，四川省凉山州人，汉族，硕士，主要从事口腔种植学钛种植体表面改性研究。
  • 基金资助:
    四川省科技厅重点研发项目(22YFS0634)，项目负责人：兰小蓉；泸州市科技局重点研发计划(面上)项目(2022-SYF-33)，项目负责人：李广文；西南医科大学自然科学重点项目(2022ZD015)，项目负责人：李广文；西南医科大学附属口腔医院院级重点项目(2022Z01)，项目负责人：李广文；四川省自然科学基金面上项目(2024NSFSC0680)，项目负责人：李广文

Gold nanoparticle @ mesoporous silica modified titanium implants promote osteogenic differentiation under high glucose conditions

Deng Yunyi1, 2, 3, Chen Shichao1, 2, 3, Luo Mingdong2, 3, Li Ruotong2, 3, Lan Xiaorong2, 3, Yu Ke1, 2, 3, Li Guangwen1, 2, 3   

  1. 1Department of Implantology, Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, Sichuan Province, China; 3Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2024-03-20 Accepted:2024-05-25 Online:2025-08-08 Published:2024-12-05
  • Contact: Li Guangwen, Doctoral candidate, Attending physician, Department of Implantology, Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China Yu Ke, MD, Associate professor, Department of Implantology, Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Deng Yunyi, Master, Department of Implantology, Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:
    Sichuan Provincial Science and Technology Department Key Research and Development Project, No. 22YFS0634 (to LXR); Luzhou Science and Technology Bureau Key Research and Development Plan (Surface), No. 2022-SYF-33 (to LGW); Natural Science Key Project of Southwest Medical University, No. 2022ZD015 (to LGW); Hospital Level Key Project of Stomatology Hospital Affiliated to Southwest Medical University, No. 2022Z01 (to LGW); Natural Science Foundation of Sichuan Province (General Program), No. 2024NSFSC0680 (to LGW)

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摘要：


文题释义：

纳米金颗粒@介孔二氧化硅：将介孔二氧化硅与纳米金颗粒溶液按一定比例混合，摇匀12 h形成的均匀混悬液，是一种以纳米金颗粒为核的介孔二氧化硅纳米颗粒，兼具两种纳米粒子的生物学活性并有自身独特的形态结构，能够在高糖环境下促进骨髓间充质干细胞在钛表面的黏附和成骨分化。

二氧化钛纳米管阵列：通过阳极氧化技术在纯钛表面形成紧密排列的、参数可控的二氧化钛小管，具有良好的生物相容性及优异的理化性能，可促进成骨细胞黏附及促进成骨分化。二氧化钛纳米管腔内部可作为药物的装载容器缓释药物，可进一步提升钛种植体表面改性效果。


背景：微纳米结构改性是钛种植体表面处理的热点研究领域。糖尿病高血糖环境会影响钛种植体与骨组织形成稳定的结合，探索通过表面微纳结构改性来提高钛种植体在高糖环境下的成骨活性是有必要的。

目的：观察钛表面纳米金颗粒@介孔二氧化硅涂层对体外高糖条件下成骨分化的影响。
方法：分别制备纳米金颗粒悬浮液与介孔二氧化硅，将二者按一定比例混合在去离子水中，制备纳米金颗粒@介孔二氧化硅悬浮液。取钛片，分3组处理：光滑组经过水砂纸打磨处理，纳米管组经过水砂纸打磨处理后采用阳极氧化技术制备二氧化钛纳米管涂层，实验组制备二氧化钛纳米管涂层后浸泡于纳米金颗粒@介孔二氧化硅悬浮液中，制备纳米金颗粒@介孔二氧化硅涂层，表征3组钛片表面的微观形貌、亲水性。将大鼠骨髓间充质干细胞接种于3组钛片表面，通过细胞活/死荧光染色及CCK-8实验检测细胞增殖，DAPI/鬼笔环肽染色检测细胞黏附。将大鼠骨髓间充质干细胞接种于3组钛片表面，加入高糖成骨诱导培养基培养，通过碱性磷酸酶与茜素红S染色检测成骨分化。

结果与结论：①扫描电镜下可见光滑组钛片表面均匀平坦，纳米管组钛片表面排列紧密的二氧化钛纳米管阵列，实验组钛片在二氧化钛纳米管表面及内部均负载纳米金颗粒@介孔二氧化硅；纳米管组、实验组钛片亲水性均优于光滑组。②细胞活/死荧光染色结果显示，3组钛片表面的细胞活力均高于90%；CCK-8实验结果显示，实验组细胞增殖率高于光滑组、纳米管组；DAPI/鬼笔环肽染色结果显示，二氧化钛纳米管涂层、纳米金颗粒@介孔二氧化硅涂层更有利于细胞黏附。③碱性磷酸酶与茜素红S染色结果显示，实验组钛片表面细胞碱性磷酸酶活性与细胞外基质矿化程度均高于光滑组、纳米管组。④结果表明，纳米金颗粒@介孔二氧化硅涂层可增强钛表面生物活性，促进高糖环境下的成骨分化。

https://orcid.org/0009-0000-8754-0357 (邓云艺) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 纳米金颗粒, 介孔二氧化硅, 钛表面改性, 微纳结构, 高糖条件, 成骨分化

Abstract: BACKGROUND: Titanium surface micro-nano structure modification is a hot research field in titanium implant surface treatment. The diabetic hyperglycemia environment will affect the stable bonding between titanium implant and bone tissue, so it is necessary to explore the surface micro-nano structure modification to improve the osteogenic activity of titanium implant in high glucose environment.
OBJECTIVE: To investigate the effect of gold nanoparticle@mesoporous silica nanoparticles (AuNPs@MSNs) coating on osteogenic activity of osteoblasts under high glucose in vitro. 
METHODS: Gold nanoparticle suspension and mesoporous silica were prepared respectively, and the two were mixed in deionized water in a certain proportion to prepare gold nanoparticle @ mesoporous silica suspension. Titanium sheets were taken and divided into three groups for treatment: the smooth group was treated with water sandpaper; the nanotube group was treated with water sandpaper and then anodized to prepare titanium dioxide nanotube coating, and the experimental group prepared titanium dioxide nanotube coating and then immersed in gold nanoparticle @ mesoporous silica suspension to prepare gold nanoparticle @ mesoporous silica nanoparticles coating. The microscopic morphology and hydrophilicity of the surface of the three groups of titanium sheets were characterized. Rat bone marrow mesenchymal stem cells were inoculated on the surface of the three groups of titanium sheets. Cell proliferation was detected by cell live/dead fluorescence staining and CCK-8 assay. Cell adhesion was detected by DAPI/phalloidin staining. Rat bone marrow mesenchymal stem cells were inoculated on the surface of the three groups of titanium sheets, and high-glucose osteogenic induction medium was added for culture. Osteogenic differentiation was detected by alkaline phosphatase and Alizarin Red S staining. 
RESULTS AND CONCLUSION: (1) Scanning electron microscopy showed that the surface of the titanium sheet in the smooth group was uniform and flat. The titanium dioxide nanotube arrays in the nanotube group were closely arranged on the surface, and the titanium sheet in the experimental group was loaded with gold nanoparticle @ mesoporous silica on the surface and inside of the titanium dioxide nanotubes. The hydrophilicity of the titanium sheets in the nanotube group and the experimental group was better than that in the smooth group. (2) The results of cell live/dead fluorescence staining exhibited that the cell viability on the surface of the three groups of titanium sheets was higher than 90%. The results of CCK-8 assay show that the cell proliferation rate in the experimental group was higher than that in the smooth group and the nanotube group. The results of DAPI/phalloidin staining showed that the titanium dioxide nanotube coating and the gold nanoparticle @ mesoporous silica nanoparticles coating were more conducive to cell adhesion. (3) The results of alkaline phosphatase and Alizarin Red S staining showed that the alkaline phosphatase activity and extracellular matrix mineralization of the cells on the titanium sheet surface in the experimental group were higher than those in the smooth group and the nanotube group. (4) The results show that the gold nanoparticle @ mesoporous silica nanoparticles coating can enhance the biological activity of the titanium surface and promote osteogenic differentiation in a high glucose environment.  

Key words: gold nanoparticle, mesoporous silica, titanium surface modification, micro-nano structure, high glucose condition, osteogenic differentiation

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