中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (16): 3380-3387.doi: 10.12307/2025.423

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

构建聚己内酯/纳米金刚石-磷脂复合材料及性能评价

田家宇1，2，李多华1，2，张  锋1，2，冯  虎2，孙  伟2   

  1. 1徐州医科大学，江苏省徐州市   221000；2徐州医科大学附属医院骨科，江苏省徐州市   221000
  • 收稿日期:2024-02-06 接受日期:2024-04-03 出版日期:2025-06-08 发布日期:2024-09-03
  • 通讯作者: 孙伟，副教授，副主任医师，硕士生导师，徐州医科大学附属医院骨科，江苏省徐州市 221000
  • 作者简介:田家宇，男，1998年生，山东省枣庄市人，汉族，硕士在读，主要从事骨外科学临床与基础研究。
  • 基金资助:
    徐州市科技项目(KC21210)，项目负责人：孙伟；徐州市医学青年后备人才培养项目(XWRCHT20220038)，项目负责人：孙伟

Construction and performance evaluation of polycaprolactone/nanodiamond-phospholipid composite materials

Tian Jiayu1, 2, Li Duohua1, 2, Zhang Feng1, 2, Feng Hu2, Sun Wei2   

  1. 1Xuzhou Medical University, Xuzhou 221000, Jiangsu Province, China; 2Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, Jiangsu Province, China
  • Received:2024-02-06 Accepted:2024-04-03 Online:2025-06-08 Published:2024-09-03
  • Contact: Sun Wei, Associate professor, Associate chief physician, Master’s supervisor, Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, Jiangsu Province, China
  • About author:Tian Jiayu, Master candidate, Xuzhou Medical University, Xuzhou 221000, Jiangsu Province, China; Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, Jiangsu Province, China
  • Supported by:
    Xuzhou Science and Technology Project, No. KC21210 (to SW); Xuzhou Medical Youth Reserve Talent Training Project, No. XWRCHT20220038 (to SW)

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摘要：

文题释义：
纳米金刚石：是一种碳基纳米材料，具有高生物相容性、抗菌活性、化学惰性以及优异的机械性能(包括硬度、弹性模量)，在生物医学领域受到广泛关注，已被证实可以作为纳米填料对可生物降解聚合物进行加固。
聚己内酯：是一种半结晶型聚合物，已被美国食品和药品管理局(FDA)批准应用于生物医学领域，具有良好的生物降解性和较高的安全性，在组织工程领域应用广泛。

背景：聚己内酯因具有良好的加工和降解性能在骨组织工程中得到广泛应用，但其较差的亲水性及机械强度并不能为成骨细胞提供良好的生长环境。
目的：制备聚己内酯/纳米金刚石-磷脂复合材料，评估其生物相容性和体外促成骨分化能力。
方法：使用磷脂对纳米金刚石进行改性，以聚己内酯为原材料，采用溶液浇铸法制备含不同质量比例(0%，2.5%，7.5%，10%)纳米金刚石-磷脂的聚己内酯/纳米金刚石-磷脂复合膜，使用聚己内酯/纳米金刚石膜进行对比，通过对各组材料表面形貌、元素组成、力学性能及水接触角的观察，选取理化性能较佳的复合膜。将MC3T3-E1细胞分别接种于纯聚己内酯膜、聚己内酯/7.5%纳米金刚石-磷脂膜及聚己内酯/7.5%纳米金刚石膜，检测细胞增殖、黏附及成骨分化能力。
结果与结论：①通过扫描电镜及表面元素组成证实聚己内酯/纳米金刚石-磷脂复合膜制备成功，与纯聚己内酯膜相比，聚己内酯/7.5%纳米金刚石-磷脂膜的拉伸强度提高了86.06%，弹性模量提高了54.76%，水接触角降低至70.0°，显示出良好的理化性能；②CCK-8检测结果显示，相较于纯聚己内酯膜、聚己内酯/7.5%纳米金刚石膜，聚己内酯/7.5%纳米金刚石-磷脂膜可促进MC3T3-E1细胞的增殖；鬼笔环肽染色显示，相较于聚己内酯/7.5%纳米金刚石膜，纯聚己内酯膜、聚己内酯/7.5%纳米金刚石-磷脂膜上的MC3T3-E1细胞多为菱形或纺锤形纤维状，细胞连接更紧密；碱性磷酸酶染色显示，相较于纯聚己内酯膜、聚己内酯/7.5%纳米金刚石膜，聚己内酯/7.5%纳米金刚石-磷脂膜上的MC3T3-E1细胞展现了更强的成骨分化能力；③结果表明，聚己内酯/纳米金刚石-磷脂复合材料具有良好的力学性能、亲水性、生物相容性及体外促成骨分化能力。

https://orcid.org/0009-0005-8783-8571 (田家宇) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 纳米金刚石, 聚己内酯, 磷脂, 溶液浇铸法, MC3T3-E1细胞, 成骨分化, 骨组织工程

Abstract:

BACKGROUND: Polycaprolactone has been widely used in bone tissue engineering due to its excellent processing and degradation performance, but its poor hydrophilicity and mechanical strength cannot provide a good growth environment for osteoblasts.

OBJECTIVE: To prepare polycaprolactone/nanodiamond-phospholipid composite materials, evaluate its biocompatibility and in vitro ability to promote bone differentiation.

METHODS: Nanodiamond was modified using phospholipids, and polycaprolactone was used as the raw material. Polycaprolactone/nanodiamond-phospholipid composite materials with different mass ratios (0%, 2.5%, 7.5%, and 10%) were prepared by solution casting method. Polycaprolactone/nanodiamonds were used for comparison. The surface morphology, elemental composition, mechanical properties, and water contact angle of each group of materials were observed to select composite materials with better physical and chemical properties. MC3T3-E1 cells were inoculated onto pure polycaprolactone membrane, polycaprolactone/7.5% nanodiamond-phospholipid membrane, and polycaprolactone/7.5% polycaprolactone/nanodiamond membrane, respectively, to detect cell proliferation, adhesion, and osteogenic differentiation ability. 
RESULTS AND CONCLUSION: (1) The successful preparation of the polycaprolactone/nanodiamond-phospholipid composite material was confirmed by scanning electron microscopy and surface element composition. Compared with pure polycaprolactone membrane, the tensile strength of the polycaprolactone/7.5% nanodiamond-phospholipids membrane increased by 86.06%, the elastic modulus increased by 54.76%, and the water contact angle decreased to 70.0°, showing good physical and chemical properties. (2) The CCK-8 assay results showed that compared with pure polycaprolactone membrane and polycaprolactone/7.5% nanodiamond membrane, polycaprolactone/7.5% nanodiamond-phospholipid membrane could promote the proliferation of MC3T3-E1 cells. Phalloidine staining exhibited that compared with the polycaprolactone/7.5% nanodiamond membrane, the MC3T3-E1 cells on pure polycaprolactone and polycaprolactone/7.5% nanodiamond-phospholipid membrane were mostly rhomboid or spindle-shaped fibers, and the cells were more closely connected. Alkaline phosphatase staining showed that MC3T3-E1 cells on polycaprolactone/7.5% nanodiamond-phospholipid membrane exhibited stronger osteogenic differentiation ability compared with pure polycaprolactone membrane and polycaprolactone /7.5% nanodiamond membrane. (3) The results indicate that the polycaprolactone/nanodiamond-phospholipid composite material has good mechanical properties, hydrophilicity, biocompatibility, and the ability to promote osteogenic differentiation in vitro.

Key words: nanodiamond, polycaprolactone, phospholipid, solution casting method, MC3T3-E1 cell, osteogenic differentiation, bone tissue engineering

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